Incidental Mutation 'R6256:Zfy1'
ID 506156
Institutional Source Beutler Lab
Gene Symbol Zfy1
Ensembl Gene ENSMUSG00000053211
Gene Name zinc finger protein 1, Y-linked
Synonyms Zfy-1
MMRRC Submission 044373-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.047) question?
Stock # R6256 (G1)
Quality Score 221.999
Status Validated
Chromosome Y
Chromosomal Location 725207-797409 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 738765 bp (GRCm39)
Zygosity Homozygous
Amino Acid Change Valine to Glutamic Acid at position 147 (V147E)
Ref Sequence ENSEMBL: ENSMUSP00000140600 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000065545] [ENSMUST00000189888]
AlphaFold P10925
Predicted Effect unknown
Transcript: ENSMUST00000065545
AA Change: V147E
SMART Domains Protein: ENSMUSP00000069364
Gene: ENSMUSG00000053211
AA Change: V147E

DomainStartEndE-ValueType
low complexity region 3 13 N/A INTRINSIC
Pfam:Zfx_Zfy_act 68 388 1.1e-109 PFAM
ZnF_C2H2 403 425 1.95e-3 SMART
ZnF_C2H2 434 456 1.26e-2 SMART
ZnF_C2H2 466 488 1.05e1 SMART
ZnF_C2H2 497 520 1.41e0 SMART
ZnF_C2H2 526 548 3.69e-4 SMART
ZnF_C2H2 554 577 3.63e-3 SMART
ZnF_C2H2 583 605 8.98e0 SMART
ZnF_C2H2 611 634 3.58e-2 SMART
ZnF_C2H2 640 662 2.95e-3 SMART
ZnF_C2H2 668 691 3.47e0 SMART
ZnF_C2H2 697 719 1.45e-2 SMART
ZnF_C2H2 725 748 1.2e-3 SMART
ZnF_C2H2 754 776 5.81e-2 SMART
Predicted Effect unknown
Transcript: ENSMUST00000189888
AA Change: V147E
SMART Domains Protein: ENSMUSP00000140600
Gene: ENSMUSG00000053211
AA Change: V147E

DomainStartEndE-ValueType
low complexity region 3 13 N/A INTRINSIC
Pfam:Zfx_Zfy_act 67 388 1.2e-141 PFAM
ZnF_C2H2 403 425 1.95e-3 SMART
ZnF_C2H2 434 456 1.26e-2 SMART
ZnF_C2H2 466 488 1.05e1 SMART
ZnF_C2H2 497 520 1.41e0 SMART
ZnF_C2H2 526 548 3.69e-4 SMART
ZnF_C2H2 554 577 3.63e-3 SMART
ZnF_C2H2 583 605 8.98e0 SMART
ZnF_C2H2 611 634 3.58e-2 SMART
ZnF_C2H2 640 662 2.95e-3 SMART
ZnF_C2H2 668 691 3.47e0 SMART
ZnF_C2H2 697 719 1.45e-2 SMART
ZnF_C2H2 725 748 1.2e-3 SMART
ZnF_C2H2 754 776 5.81e-2 SMART
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 97.9%
  • 20x: 93.5%
Validation Efficiency 99% (74/75)
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700030K09Rik A T 8: 73,205,272 (GRCm39) Q361L probably damaging Het
Abca7 A G 10: 79,838,456 (GRCm39) T577A probably damaging Het
Acad12 A T 5: 121,752,149 (GRCm39) V54E probably benign Het
Ago4 A G 4: 126,414,019 (GRCm39) Y91H probably damaging Het
Akr1b10 T C 6: 34,364,623 (GRCm39) V28A probably damaging Het
Ccdc33 T A 9: 58,009,201 (GRCm39) probably null Het
Ccdc7a T C 8: 129,662,074 (GRCm39) probably null Het
Ces1f A T 8: 93,992,422 (GRCm39) V343E probably damaging Het
Cftr T C 6: 18,274,660 (GRCm39) L896P probably damaging Het
Csmd3 G A 15: 47,533,125 (GRCm39) P2375S probably damaging Het
Dnajb14 C T 3: 137,614,124 (GRCm39) A345V probably damaging Het
Dnajb14 G C 3: 137,614,123 (GRCm39) A345P probably damaging Het
Dnase1 G A 16: 3,855,485 (GRCm39) R24K probably benign Het
Dnmbp C T 19: 43,840,720 (GRCm39) V560M probably damaging Het
Dop1b T A 16: 93,604,102 (GRCm39) I1981N possibly damaging Het
Eif3a A C 19: 60,759,464 (GRCm39) S770A possibly damaging Het
Fbxl7 A T 15: 26,553,088 (GRCm39) C60S probably benign Het
Fras1 A T 5: 96,881,702 (GRCm39) D2478V possibly damaging Het
Hrnr A G 3: 93,229,918 (GRCm39) D52G probably damaging Het
Jmjd1c T A 10: 67,056,187 (GRCm39) L823M probably damaging Het
Kdm1a G T 4: 136,295,911 (GRCm39) C172* probably null Het
Kdm6b C T 11: 69,297,555 (GRCm39) E295K probably damaging Het
Mepe A T 5: 104,484,940 (GRCm39) M27L probably benign Het
Mogat2 A T 7: 98,869,102 (GRCm39) H305Q probably damaging Het
Mst1r T A 9: 107,794,465 (GRCm39) Y1215N probably damaging Het
Muc5ac A T 7: 141,343,532 (GRCm39) H48L possibly damaging Het
Myo7b T C 18: 32,116,748 (GRCm39) D953G probably damaging Het
Ocel1 T C 8: 71,824,472 (GRCm39) probably benign Het
Pcdhb6 A G 18: 37,468,978 (GRCm39) D633G probably damaging Het
Ppm1l A G 3: 69,405,230 (GRCm39) I176V probably benign Het
Sall3 C T 18: 81,013,076 (GRCm39) R1120H possibly damaging Het
Sbf1 G A 15: 89,185,070 (GRCm39) P1018S probably benign Het
Setbp1 T C 18: 78,900,472 (GRCm39) Y1065C probably damaging Het
Slc25a2 T C 18: 37,770,776 (GRCm39) probably null Het
Slc4a2 C A 5: 24,640,888 (GRCm39) T729K probably damaging Het
Sptlc2 T C 12: 87,402,305 (GRCm39) E207G probably damaging Het
Sult6b1 A T 17: 79,214,343 (GRCm39) F27I probably benign Het
Syf2 A T 4: 134,661,889 (GRCm39) K84N probably damaging Het
Tmem209 A T 6: 30,497,166 (GRCm39) N183K probably benign Het
Tmem232 G T 17: 65,785,397 (GRCm39) Q188K possibly damaging Het
Tomm70a A T 16: 56,973,055 (GRCm39) T598S probably benign Het
Ttll13 A G 7: 79,908,052 (GRCm39) T556A probably benign Het
Vmn2r120 A T 17: 57,831,700 (GRCm39) L363* probably null Het
Xpo6 G T 7: 125,707,791 (GRCm39) Q872K probably damaging Het
Xrra1 A C 7: 99,563,671 (GRCm39) S553R probably damaging Het
Zfy2 T C Y: 2,116,267 (GRCm39) I258V probably benign Homo
Other mutations in Zfy1
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0149:Zfy1 UTSW Y 726,121 (GRCm39) missense possibly damaging 0.95
R0361:Zfy1 UTSW Y 726,121 (GRCm39) missense possibly damaging 0.95
R0529:Zfy1 UTSW Y 726,040 (GRCm39) missense probably damaging 1.00
R0837:Zfy1 UTSW Y 725,850 (GRCm39) nonsense probably null
R0945:Zfy1 UTSW Y 725,983 (GRCm39) missense probably damaging 0.98
R1163:Zfy1 UTSW Y 725,611 (GRCm39) missense probably damaging 0.98
R1394:Zfy1 UTSW Y 725,957 (GRCm39) missense possibly damaging 0.79
R1806:Zfy1 UTSW Y 725,620 (GRCm39) missense possibly damaging 0.88
R1928:Zfy1 UTSW Y 729,733 (GRCm39) missense unknown
R2374:Zfy1 UTSW Y 726,392 (GRCm39) missense possibly damaging 0.77
R2374:Zfy1 UTSW Y 726,391 (GRCm39) missense probably damaging 0.99
R2889:Zfy1 UTSW Y 726,307 (GRCm39) missense possibly damaging 0.95
R2980:Zfy1 UTSW Y 739,054 (GRCm39) missense unknown
R3437:Zfy1 UTSW Y 726,357 (GRCm39) missense possibly damaging 0.82
R4454:Zfy1 UTSW Y 725,518 (GRCm39) missense possibly damaging 0.86
R4529:Zfy1 UTSW Y 726,511 (GRCm39) missense possibly damaging 0.93
R4656:Zfy1 UTSW Y 729,626 (GRCm39) missense unknown
R5049:Zfy1 UTSW Y 726,004 (GRCm39) missense possibly damaging 0.93
R5087:Zfy1 UTSW Y 732,964 (GRCm39) missense unknown
R5347:Zfy1 UTSW Y 725,950 (GRCm39) missense possibly damaging 0.90
R5428:Zfy1 UTSW Y 726,205 (GRCm39) missense possibly damaging 0.95
R5825:Zfy1 UTSW Y 726,531 (GRCm39) missense possibly damaging 0.85
R7065:Zfy1 UTSW Y 725,428 (GRCm39) missense probably benign 0.33
R7134:Zfy1 UTSW Y 725,788 (GRCm39) missense probably damaging 0.99
R7185:Zfy1 UTSW Y 725,464 (GRCm39) missense possibly damaging 0.53
R7358:Zfy1 UTSW Y 735,141 (GRCm39) missense unknown
R7513:Zfy1 UTSW Y 759,852 (GRCm39) missense unknown
R7747:Zfy1 UTSW Y 725,496 (GRCm39) nonsense probably null
R7900:Zfy1 UTSW Y 725,519 (GRCm39) missense possibly damaging 0.53
R8052:Zfy1 UTSW Y 726,004 (GRCm39) missense possibly damaging 0.93
R8377:Zfy1 UTSW Y 725,723 (GRCm39) missense possibly damaging 0.90
R8795:Zfy1 UTSW Y 738,945 (GRCm39) missense unknown
R8854:Zfy1 UTSW Y 726,501 (GRCm39) missense possibly damaging 0.71
R8936:Zfy1 UTSW Y 738,726 (GRCm39) missense unknown
R9098:Zfy1 UTSW Y 725,987 (GRCm39) missense possibly damaging 0.95
R9146:Zfy1 UTSW Y 726,033 (GRCm39) missense possibly damaging 0.95
R9209:Zfy1 UTSW Y 732,990 (GRCm39) missense unknown
R9310:Zfy1 UTSW Y 727,634 (GRCm39) missense unknown
R9726:Zfy1 UTSW Y 725,476 (GRCm39) missense possibly damaging 0.53
Predicted Primers PCR Primer
(F):5'- ATGTGGCTCAAGACTTACAAGAC -3'
(R):5'- CCAGGCTCAGTTATAATTCAAGATG -3'

Sequencing Primer
(F):5'- CATCATCAGATAATCCTCACAGTTG -3'
(R):5'- CTGACAATGTCATTATTCCTGAGC -3'
Posted On 2018-02-28