Mutagenetix > Incidental Mutation

Incidental Mutation 'R9562:Top2b'

721136

Institutional Source

Beutler Lab

Gene Symbol

Top2b

Ensembl Gene

ENSMUSG00000017485

Gene Name

topoisomerase (DNA) II beta

Synonyms

D230016L12Rik, Top-2

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.888) question?

Stock #

R9562 (G1)

Quality Score

127.008

Status

Not validated

Chromosome

Chromosomal Location

16365179-16435462 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 16365718 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 14 (T14P)

Ref Sequence

ENSEMBL: ENSMUSP00000017629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000017629]

AlphaFold

Q64511

Predicted Effect

probably benign
Transcript: ENSMUST00000017629
AA Change: T14P

PolyPhen 2 Score 0.094 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000017629
Gene: ENSMUSG00000017485
AA Change: T14P

Domain	Start	End	E-Value	Type
Blast:TOP2c	32	70	7e-10	BLAST
HATPase_c	85	234	1.91e-2	SMART
TOP2c	89	679	N/A	SMART
TOP4c	702	1175	2.55e-230	SMART
low complexity region	1201	1215	N/A	INTRINSIC
low complexity region	1287	1299	N/A	INTRINSIC
low complexity region	1324	1336	N/A	INTRINSIC
low complexity region	1360	1382	N/A	INTRINSIC
Pfam:DTHCT	1495	1597	4.6e-31	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
PHENOTYPE: Homozygous null mice exhibit abnormal innervation. Offspring die shortly after birth due to respiratory failure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 129 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	T	C	6: 48,930,975 (GRCm38)	M303T	probably benign	Het
2310035C23Rik	T	C	1: 105,664,151 (GRCm38)	V15A	probably damaging	Het
Acacb	C	A	5: 114,233,336 (GRCm38)	P1842Q	probably damaging	Het
Aco2	G	A	15: 81,889,434 (GRCm38)	R58Q	probably null	Het
Acvr1	T	C	2: 58,448,373 (GRCm38)	K446E	probably damaging	Het
Adam39	T	C	8: 40,824,718 (GRCm38)	Y49H	probably benign	Het
Afg3l2	C	T	18: 67,421,295 (GRCm38)	V446M	probably damaging	Het
Aire	A	G	10: 78,035,745 (GRCm38)	V397A	probably benign	Het
Ankdd1a	C	T	9: 65,504,170 (GRCm38)	A352T	possibly damaging	Het
Arfip2	T	C	7: 105,636,872 (GRCm38)	D270G	possibly damaging	Het
Arl4c	A	G	1: 88,701,412 (GRCm38)	C85R	probably damaging	Het
Arpp21	A	C	9: 112,127,286 (GRCm38)	V541G	possibly damaging	Het
Atp2a3	T	A	11: 72,982,752 (GRCm38)	M817K	probably damaging	Het
Bahcc1	C	T	11: 120,259,209 (GRCm38)	H106Y	possibly damaging	Het
BC067074	T	C	13: 113,368,040 (GRCm38)	I1901T		Het
Bcl9l	A	G	9: 44,500,779 (GRCm38)	S21G	possibly damaging	Het
C7	T	A	15: 5,057,097 (GRCm38)		probably null	Het
Cabcoco1	T	C	10: 68,436,895 (GRCm38)	D220G	possibly damaging	Het
Cacna1e	T	A	1: 154,407,740 (GRCm38)	D1960V	probably benign	Het
Casp4	A	T	9: 5,324,832 (GRCm38)	T215S	probably damaging	Het
Ccdc187	T	C	2: 26,293,686 (GRCm38)	S101G	possibly damaging	Het
Cdk12	A	G	11: 98,249,802 (GRCm38)	N1290D	unknown	Het
Cdo1	C	A	18: 46,728,037 (GRCm38)	V36L	probably benign	Het
Col5a3	G	A	9: 20,803,133 (GRCm38)	A381V	unknown	Het
Dcpp2	A	G	17: 23,900,479 (GRCm38)	E89G	probably damaging	Het
Dhx57	G	T	17: 80,254,388 (GRCm38)	A899E	probably damaging	Het
Dnaaf1	T	C	8: 119,582,653 (GRCm38)	I160T	probably damaging	Het
Dnah1	C	A	14: 31,264,437 (GRCm38)	A3698S	probably damaging	Het
Dnah12	C	A	14: 26,875,324 (GRCm38)	R3547S	possibly damaging	Het
Dnah3	A	G	7: 120,010,891 (GRCm38)	V1774A	probably benign	Het
Dopey1	G	A	9: 86,542,758 (GRCm38)	R2085Q	probably damaging	Het
Duox1	T	C	2: 122,320,722 (GRCm38)	Y293H	probably damaging	Het
Efcab5	G	A	11: 77,132,108 (GRCm38)	T593I	probably damaging	Het
Epas1	T	A	17: 86,805,239 (GRCm38)	D88E	probably damaging	Het
Epdr1	A	G	13: 19,594,651 (GRCm38)	F92L	possibly damaging	Het
Ercc6	T	C	14: 32,574,967 (GRCm38)	W1305R	probably damaging	Het
Etv6	A	G	6: 134,248,709 (GRCm38)	N212S	probably benign	Het
Fam208a	T	C	14: 27,479,809 (GRCm38)		probably null	Het
Fam20b	A	T	1: 156,702,440 (GRCm38)	Y141*	probably null	Het
Fancm	A	G	12: 65,121,720 (GRCm38)	N1619S	probably damaging	Het
Fbln5	A	T	12: 101,768,463 (GRCm38)	N183K	probably damaging	Het
Fer1l6	A	G	15: 58,618,521 (GRCm38)	D976G	possibly damaging	Het
Fgb	T	C	3: 83,045,102 (GRCm38)		probably null	Het
Fhod1	A	T	8: 105,347,790 (GRCm38)	Y22N	unknown	Het
Galm	C	T	17: 80,183,430 (GRCm38)	Q166*	probably null	Het
Gbe1	A	T	16: 70,401,776 (GRCm38)	Y119F	probably benign	Het
Gcnt7	T	A	2: 172,451,260 (GRCm38)	Y369F	probably damaging	Het
Gdap2	T	C	3: 100,191,690 (GRCm38)	I366T	possibly damaging	Het
Gm11236	G	T	4: 73,638,953 (GRCm38)	V8F	probably damaging	Het
Gm14295	T	C	2: 176,807,369 (GRCm38)	V4A	probably benign	Het
Gm32742	T	A	9: 51,157,027 (GRCm38)	Y249F	probably benign	Het
Gm40460	A	T	7: 142,240,964 (GRCm38)	C39S	unknown	Het
Gm5294	C	A	5: 138,821,458 (GRCm38)	Q164K	probably benign	Het
Gm6370	T	A	5: 146,492,471 (GRCm38)	C120*	probably null	Het
Harbi1	T	A	2: 91,712,353 (GRCm38)	L53*	probably null	Het
Herc3	A	G	6: 58,859,014 (GRCm38)	N280S	probably null	Het
Hipk2	T	A	6: 38,747,455 (GRCm38)	R447W	probably damaging	Het
Hydin	A	G	8: 110,586,154 (GRCm38)	I4176V	probably benign	Het
Ifi211	A	T	1: 173,905,486 (GRCm38)	I203N	probably benign	Het
Ikbkap	G	T	4: 56,772,521 (GRCm38)	P867T	probably benign	Het
Kcnc1	G	A	7: 46,427,586 (GRCm38)	V271M	probably benign	Het
Kcng1	T	A	2: 168,262,877 (GRCm38)	I350F	probably damaging	Het
Kif21b	A	T	1: 136,149,352 (GRCm38)	I371F	probably damaging	Het
Klrc1	A	G	6: 129,678,783 (GRCm38)	F43S	probably damaging	Het
Krtap6-2	A	G	16: 89,419,834 (GRCm38)	S82P	unknown	Het
Lamb1	T	A	12: 31,272,493 (GRCm38)	M186K	probably damaging	Het
Lrig2	T	C	3: 104,468,608 (GRCm38)	K451E	possibly damaging	Het
Lrrc15	G	A	16: 30,274,198 (GRCm38)	L108F	probably damaging	Het
Mark3	A	G	12: 111,604,526 (GRCm38)	I87V	probably damaging	Het
Mgea5	CTCGGGTC	CTC	19: 45,754,657 (GRCm38)		probably null	Het
Mlh1	T	C	9: 111,230,945 (GRCm38)	K622E		Het
Mrgprb2	G	A	7: 48,552,926 (GRCm38)	T17M	possibly damaging	Het
Mtmr4	C	T	11: 87,602,415 (GRCm38)	R225C	probably damaging	Het
Nfatc1	A	G	18: 80,635,701 (GRCm38)	L824P	probably damaging	Het
Nlrp4f	A	T	13: 65,199,239 (GRCm38)	D46E	probably damaging	Het
Olfr1564	G	T	17: 33,215,441 (GRCm38)	T301N	probably benign	Het
Olfr225	T	A	11: 59,613,754 (GRCm38)	N263K	probably damaging	Het
Olfr365	G	T	2: 37,201,563 (GRCm38)	M107I	probably benign	Het
Olfr5	G	C	7: 6,480,244 (GRCm38)	A304G	probably null	Het
Olfr702	A	C	7: 106,823,813 (GRCm38)	F238V	probably damaging	Het
Olfr866	T	A	9: 20,027,749 (GRCm38)	Y63F	probably damaging	Het
Olfr906	G	T	9: 38,488,796 (GRCm38)	A256S	possibly damaging	Het
Otulin	A	G	15: 27,608,726 (GRCm38)	Y244H	probably damaging	Het
Pabpc4	T	C	4: 123,286,860 (GRCm38)	I125T	probably damaging	Het
Parp14	A	G	16: 35,857,405 (GRCm38)	I731T	probably benign	Het
Parp8	T	C	13: 116,893,095 (GRCm38)	T531A	probably benign	Het
Parvg	G	T	15: 84,328,864 (GRCm38)	V49L	probably benign	Het
Pcdha12	T	C	18: 37,022,231 (GRCm38)	S668P	probably damaging	Het
Pcdhb9	A	C	18: 37,401,612 (GRCm38)	N220H	probably benign	Het
Pcdhga4	T	G	18: 37,686,474 (GRCm38)	S359A	probably benign	Het
Pi4k2b	T	A	5: 52,751,457 (GRCm38)	S208R	probably damaging	Het
Plxnb2	T	C	15: 89,165,933 (GRCm38)	Y487C	probably damaging	Het
Pm20d1	G	A	1: 131,802,763 (GRCm38)	V252I	probably damaging	Het
Pxdc1	T	A	13: 34,652,275 (GRCm38)	H54L	probably damaging	Het
Rag1	A	T	2: 101,642,982 (GRCm38)	V605E	probably damaging	Het
Rasgrf2	A	T	13: 91,886,350 (GRCm38)		probably null	Het
Rflnb	T	A	11: 76,022,125 (GRCm38)	T146S	probably damaging	Het
Rfx5	T	A	3: 94,959,328 (GRCm38)	L646Q	unknown	Het
Rnf43	A	G	11: 87,728,065 (GRCm38)	T81A	probably benign	Het
Ros1	T	C	10: 52,067,074 (GRCm38)	D2048G	probably damaging	Het
Rpl13a-ps1	T	A	19: 50,030,173 (GRCm38)	K188I	possibly damaging	Het
Rpl24	T	A	16: 55,970,146 (GRCm38)	S122T	probably benign	Het
Ryr2	G	A	13: 11,745,218 (GRCm38)	T1549M	probably damaging	Het
Sbf2	T	C	7: 110,441,495 (GRCm38)	Q375R	possibly damaging	Het
Sec23a	A	T	12: 59,002,031 (GRCm38)	F131I	possibly damaging	Het
Secisbp2	T	C	13: 51,683,284 (GRCm38)	I827T	probably damaging	Het
Serpinb6d	A	G	13: 33,670,773 (GRCm38)	R244G	probably benign	Het
Skor2	C	T	18: 76,858,681 (GRCm38)	H33Y	unknown	Het
Snx17	C	A	5: 31,197,744 (GRCm38)	Q368K	probably damaging	Het
Stac	T	A	9: 111,572,343 (GRCm38)	E345V	probably benign	Het
Stx4a	A	G	7: 127,846,203 (GRCm38)	N164S	possibly damaging	Het
Tbcb	A	T	7: 30,231,124 (GRCm38)		probably null	Het
Tecpr2	T	C	12: 110,947,707 (GRCm38)	S1151P	possibly damaging	Het
Timm50	A	G	7: 28,307,644 (GRCm38)	F202S	possibly damaging	Het
Tle2	A	G	10: 81,581,733 (GRCm38)	D244G	probably benign	Het
Tlr4	G	A	4: 66,841,285 (GRCm38)	V772I	possibly damaging	Het
Tmem214	G	A	5: 30,869,699 (GRCm38)	W11*	probably null	Het
Tmem241	A	T	18: 12,043,299 (GRCm38)	L227*	probably null	Het
Trim44	T	C	2: 102,357,482 (GRCm38)	T309A	probably benign	Het
Trps1	A	C	15: 50,661,261 (GRCm38)	I1091S	probably damaging	Het
Tspan2	C	A	3: 102,765,267 (GRCm38)	T200K	probably damaging	Het
Tuba8	A	G	6: 121,223,104 (GRCm38)	N249S	probably benign	Het
Vmn1r189	A	G	13: 22,102,256 (GRCm38)	L137P	probably damaging	Het
Vmn2r29	A	T	7: 7,241,856 (GRCm38)	W340R	probably benign	Het
Vmn2r52	A	C	7: 10,159,549 (GRCm38)	D554E	probably benign	Het
Zc3h4	C	G	7: 16,434,966 (GRCm38)	P1075R	unknown	Het
Zfp599	A	G	9: 22,249,703 (GRCm38)	S389P	probably damaging	Het
Zfp777	A	T	6: 48,044,646 (GRCm38)	V14D	possibly damaging	Het
Zswim8	C	T	14: 20,712,082 (GRCm38)	Q262*	probably null	Het

Other mutations in Top2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00430:Top2b	APN	14	16,422,692 (GRCm38)	missense	probably benign	0.00
IGL00730:Top2b	APN	14	16,389,831 (GRCm38)	missense	probably damaging	1.00
IGL00917:Top2b	APN	14	16,407,354 (GRCm38)	missense	probably benign	0.05
IGL01959:Top2b	APN	14	16,422,695 (GRCm38)	missense	probably benign	0.19
IGL02019:Top2b	APN	14	16,409,965 (GRCm38)	missense	probably benign	0.44
IGL02119:Top2b	APN	14	16,406,733 (GRCm38)	missense	probably damaging	1.00
IGL02136:Top2b	APN	14	16,407,103 (GRCm38)	unclassified	probably benign
IGL02148:Top2b	APN	14	16,400,488 (GRCm38)	missense	probably damaging	1.00
IGL02496:Top2b	APN	14	16,387,335 (GRCm38)	missense	probably benign
IGL02503:Top2b	APN	14	16,407,163 (GRCm38)	missense	possibly damaging	0.92
IGL02672:Top2b	APN	14	16,409,166 (GRCm38)	unclassified	probably benign
IGL02721:Top2b	APN	14	16,409,236 (GRCm38)	missense	probably damaging	1.00
IGL02886:Top2b	APN	14	16,365,688 (GRCm38)	missense	possibly damaging	0.73
IGL03252:Top2b	APN	14	16,393,163 (GRCm38)	missense	possibly damaging	0.60
PIT4434001:Top2b	UTSW	14	16,423,780 (GRCm38)	critical splice donor site	probably null
R0092:Top2b	UTSW	14	16,409,263 (GRCm38)	missense	probably damaging	1.00
R0201:Top2b	UTSW	14	16,383,174 (GRCm38)	missense	probably damaging	1.00
R0390:Top2b	UTSW	14	16,418,442 (GRCm38)	missense	probably benign	0.00
R0394:Top2b	UTSW	14	16,413,556 (GRCm38)	splice site	probably null
R1159:Top2b	UTSW	14	16,430,329 (GRCm38)	missense	possibly damaging	0.81
R1424:Top2b	UTSW	14	16,383,177 (GRCm38)	missense	probably damaging	1.00
R1519:Top2b	UTSW	14	16,408,953 (GRCm38)	splice site	probably null
R1561:Top2b	UTSW	14	16,398,993 (GRCm38)	missense	possibly damaging	0.80
R1713:Top2b	UTSW	14	16,409,823 (GRCm38)	missense	probably benign	0.05
R1987:Top2b	UTSW	14	16,398,916 (GRCm38)	missense	probably damaging	0.99
R2219:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R2287:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R2422:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R2679:Top2b	UTSW	14	16,413,947 (GRCm38)	missense	probably damaging	1.00
R3687:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R3707:Top2b	UTSW	14	16,388,447 (GRCm38)	missense	probably damaging	1.00
R3810:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R3812:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R3815:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R3816:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R3818:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4023:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4025:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4026:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4133:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4157:Top2b	UTSW	14	16,384,491 (GRCm38)	missense	probably benign	0.42
R4179:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4180:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4300:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4376:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4377:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4492:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4549:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4550:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4581:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4582:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4628:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4630:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4667:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4668:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4669:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R4698:Top2b	UTSW	14	16,387,331 (GRCm38)	nonsense	probably null
R4769:Top2b	UTSW	14	16,398,991 (GRCm38)	missense	probably damaging	1.00
R4809:Top2b	UTSW	14	16,383,125 (GRCm38)	missense	probably benign	0.06
R4899:Top2b	UTSW	14	16,387,313 (GRCm38)	missense	probably damaging	1.00
R5035:Top2b	UTSW	14	16,409,966 (GRCm38)	missense	probably benign	0.01
R5621:Top2b	UTSW	14	16,387,280 (GRCm38)	missense	probably damaging	1.00
R5631:Top2b	UTSW	14	16,409,882 (GRCm38)	missense	probably damaging	1.00
R5685:Top2b	UTSW	14	16,413,666 (GRCm38)	missense	probably damaging	1.00
R5732:Top2b	UTSW	14	16,400,106 (GRCm38)	missense	possibly damaging	0.92
R5939:Top2b	UTSW	14	16,422,786 (GRCm38)	missense	probably damaging	0.96
R6007:Top2b	UTSW	14	16,423,779 (GRCm38)	critical splice donor site	probably null
R6087:Top2b	UTSW	14	16,409,864 (GRCm38)	missense	probably benign	0.14
R6144:Top2b	UTSW	14	16,423,740 (GRCm38)	missense	possibly damaging	0.48
R6196:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R6218:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R6229:Top2b	UTSW	14	16,409,838 (GRCm38)	missense	probably damaging	1.00
R6249:Top2b	UTSW	14	16,399,006 (GRCm38)	missense	probably damaging	1.00
R6337:Top2b	UTSW	14	16,399,026 (GRCm38)	missense	possibly damaging	0.77
R6353:Top2b	UTSW	14	16,416,671 (GRCm38)	missense	probably damaging	1.00
R6512:Top2b	UTSW	14	16,409,854 (GRCm38)	missense	possibly damaging	0.94
R6573:Top2b	UTSW	14	16,398,991 (GRCm38)	missense	probably damaging	1.00
R6614:Top2b	UTSW	14	16,407,142 (GRCm38)	nonsense	probably null
R6844:Top2b	UTSW	14	16,429,383 (GRCm38)	missense	possibly damaging	0.94
R6848:Top2b	UTSW	14	16,409,958 (GRCm38)	missense	possibly damaging	0.89
R6871:Top2b	UTSW	14	16,409,189 (GRCm38)	missense	probably damaging	1.00
R6895:Top2b	UTSW	14	16,413,604 (GRCm38)	missense	probably benign	0.06
R7162:Top2b	UTSW	14	16,416,653 (GRCm38)	missense	probably benign	0.00
R7247:Top2b	UTSW	14	16,416,962 (GRCm38)	missense	probably benign	0.08
R7250:Top2b	UTSW	14	16,420,411 (GRCm38)	missense	probably benign
R7359:Top2b	UTSW	14	16,407,376 (GRCm38)	missense	probably null	1.00
R7365:Top2b	UTSW	14	16,416,649 (GRCm38)	missense	probably benign	0.04
R7493:Top2b	UTSW	14	16,416,605 (GRCm38)	missense	probably benign	0.00
R7528:Top2b	UTSW	14	16,395,427 (GRCm38)	nonsense	probably null
R7562:Top2b	UTSW	14	16,412,946 (GRCm38)	missense	probably benign	0.04
R7594:Top2b	UTSW	14	16,428,587 (GRCm38)	missense	probably benign
R7670:Top2b	UTSW	14	16,416,620 (GRCm38)	missense	possibly damaging	0.61
R7894:Top2b	UTSW	14	16,413,081 (GRCm38)	missense	possibly damaging	0.68
R8031:Top2b	UTSW	14	16,412,986 (GRCm38)	missense	probably damaging	0.98
R8150:Top2b	UTSW	14	16,393,291 (GRCm38)	missense	probably damaging	0.99
R8214:Top2b	UTSW	14	16,383,177 (GRCm38)	missense	probably damaging	1.00
R8299:Top2b	UTSW	14	16,386,123 (GRCm38)	missense	possibly damaging	0.68
R8977:Top2b	UTSW	14	16,393,239 (GRCm38)	missense	probably benign	0.36
R9565:Top2b	UTSW	14	16,365,718 (GRCm38)	missense	probably benign	0.09
R9798:Top2b	UTSW	14	16,389,845 (GRCm38)	missense	probably damaging	1.00
X0028:Top2b	UTSW	14	16,384,499 (GRCm38)	nonsense	probably null
Z1176:Top2b	UTSW	14	16,395,434 (GRCm38)	missense	probably damaging	1.00
Z1177:Top2b	UTSW	14	16,416,953 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CTAGTCCTCGACCACTCTGTAG -3'
(R):5'- AGCATTGTTCAGCAGCCTG -3'

Sequencing Primer
(F):5'- CGCGCTCGAGTTTGAGG -3'
(R):5'- TCAGCAGCCTGAGCCTTG -3'

Posted On

2022-08-09