Mutagenetix > Incidental Mutation

Incidental Mutation 'R1780:Trio'

197479

Institutional Source

Beutler Lab

Gene Symbol

Trio

Ensembl Gene

ENSMUSG00000022263

Gene Name

triple functional domain (PTPRF interacting)

Synonyms

Solo, 6720464I07Rik

MMRRC Submission

039811-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1780 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

27730651-28025848 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 27744038 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 2603 (C2603R)

Ref Sequence

ENSEMBL: ENSMUSP00000087714 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090247] [ENSMUST00000226644]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000090247
AA Change: C2603R

PolyPhen 2 Score 0.934 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000087714
Gene: ENSMUSG00000022263
AA Change: C2603R

Domain	Start	End	E-Value	Type
low complexity region	2	40	N/A	INTRINSIC
SEC14	68	207	3.4e-26	SMART
SPEC	221	337	2.48e-9	SMART
SPEC	343	445	1.92e-15	SMART
SPEC	569	671	5.35e-14	SMART
SPEC	674	783	1.18e-6	SMART
SPEC	910	1011	2.6e-12	SMART
SPEC	1141	1243	7e-18	SMART
low complexity region	1249	1258	N/A	INTRINSIC
RhoGEF	1296	1466	2.79e-53	SMART
PH	1480	1593	1.53e-9	SMART
SH3	1659	1720	1.9e-8	SMART
low complexity region	1788	1802	N/A	INTRINSIC
low complexity region	1837	1863	N/A	INTRINSIC
low complexity region	1936	1954	N/A	INTRINSIC
RhoGEF	1973	2144	1.32e-63	SMART
PH	2158	2273	3.6e-6	SMART
low complexity region	2291	2341	N/A	INTRINSIC
low complexity region	2371	2390	N/A	INTRINSIC
low complexity region	2491	2503	N/A	INTRINSIC
SH3	2558	2619	1.04e0	SMART
low complexity region	2640	2660	N/A	INTRINSIC
IGc2	2701	2770	4e-12	SMART
S_TKc	2800	3054	4.84e-72	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000226644

Predicted Effect

probably benign
Transcript: ENSMUST00000226713

Predicted Effect

probably benign
Transcript: ENSMUST00000227030

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227642

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227999

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228084

Meta Mutation Damage Score

0.2366

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.3%

Validation Efficiency

98% (80/82)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Homozygous mutant mice die during late embryonic development or shortly after birth. They exhibit abnormal skeletal myogenesis and display aberrant organization within the hippocampus and olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl2	T	C	3: 148,852,593 (GRCm38)	E493G	probably damaging	Het
Aff3	A	T	1: 38,535,702 (GRCm38)	S66T	probably damaging	Het
Ankrd17	T	A	5: 90,232,415 (GRCm38)	K2470N	probably damaging	Het
Ap3m1	T	C	14: 21,041,070 (GRCm38)	T156A	probably benign	Het
Arhgef4	T	A	1: 34,724,160 (GRCm38)	S832R	possibly damaging	Het
Asb10	T	C	5: 24,533,676 (GRCm38)	D423G	possibly damaging	Het
Ash1l	A	G	3: 88,965,984 (GRCm38)	T25A	probably benign	Het
Atp13a2	T	A	4: 141,002,460 (GRCm38)	L663I	possibly damaging	Het
Atp9b	A	T	18: 80,776,897 (GRCm38)	Y174*	probably null	Het
Bche	A	G	3: 73,700,620 (GRCm38)	I491T	probably benign	Het
Bckdhb	T	C	9: 83,953,783 (GRCm38)		probably null	Het
Cadps2	C	T	6: 23,320,932 (GRCm38)		probably null	Het
Cdc42bpb	A	T	12: 111,322,907 (GRCm38)	V468E	probably damaging	Het
Chrnd	T	C	1: 87,192,548 (GRCm38)	V33A	possibly damaging	Het
Col6a5	A	T	9: 105,936,878 (GRCm38)	V645D	unknown	Het
Cpa1	C	T	6: 30,643,008 (GRCm38)	L312F	probably damaging	Het
Cyp2a5	A	G	7: 26,841,876 (GRCm38)		probably benign	Het
Cyp2c39	C	A	19: 39,538,851 (GRCm38)		probably benign	Het
Cyp2d26	T	C	15: 82,794,007 (GRCm38)	N56S	probably damaging	Het
Ddx21	A	G	10: 62,594,147 (GRCm38)		probably benign	Het
Dnah11	A	T	12: 118,027,558 (GRCm38)	C2358S	probably damaging	Het
Entpd8	T	C	2: 25,084,306 (GRCm38)	S368P	probably benign	Het
Epg5	A	T	18: 78,023,990 (GRCm38)	Q2222L	probably damaging	Het
Ercc5	T	A	1: 44,167,796 (GRCm38)	V623E	probably benign	Het
Flg2	A	G	3: 93,202,999 (GRCm38)	E778G	unknown	Het
Gbe1	C	T	16: 70,495,324 (GRCm38)	R515*	probably null	Het
Hsd17b6	A	G	10: 127,994,327 (GRCm38)		probably null	Het
Hyal6	C	T	6: 24,734,032 (GRCm38)		probably benign	Het
Ifi27l2b	A	G	12: 103,451,319 (GRCm38)	I203T	probably damaging	Het
Kcnk9	A	C	15: 72,512,401 (GRCm38)	D309E	unknown	Het
Lrp6	C	T	6: 134,464,451 (GRCm38)	R1184Q	probably damaging	Het
Mdn1	T	C	4: 32,700,103 (GRCm38)	F1399L	probably damaging	Het
Mroh2a	C	T	1: 88,230,680 (GRCm38)	R150*	probably null	Het
Mx1	T	C	16: 97,451,512 (GRCm38)	*289W	probably null	Het
Myo7b	T	C	18: 31,961,185 (GRCm38)	E1970G	probably damaging	Het
Mypn	T	C	10: 63,121,964 (GRCm38)	Y24C	probably damaging	Het
Naxe	G	C	3: 88,057,133 (GRCm38)	P167A	probably benign	Het
Nmnat2	A	T	1: 153,112,440 (GRCm38)	K272*	probably null	Het
Nmnat3	C	T	9: 98,354,111 (GRCm38)	T19M	probably damaging	Het
Olfr1275	T	C	2: 111,231,698 (GRCm38)	I32V	probably benign	Het
Olfr1412	G	A	1: 92,588,389 (GRCm38)	V20M	probably benign	Het
Olfr1474	A	G	19: 13,471,362 (GRCm38)	T131A	probably benign	Het
Olfr64	A	T	7: 103,893,555 (GRCm38)	F60Y	probably damaging	Het
Olfr66	A	G	7: 103,881,592 (GRCm38)	V217A	probably benign	Het
Pgm3	T	G	9: 86,556,204 (GRCm38)	E509D	probably damaging	Het
Phf3	A	T	1: 30,811,942 (GRCm38)	D1110E	probably damaging	Het
Pkd1	T	G	17: 24,581,569 (GRCm38)	S3062A	probably benign	Het
Pogz	A	T	3: 94,870,126 (GRCm38)	K372N	possibly damaging	Het
Pou1f1	A	G	16: 65,523,470 (GRCm38)	Y15C	probably benign	Het
Ppfia2	A	G	10: 106,896,507 (GRCm38)	T972A	possibly damaging	Het
Rasip1	A	G	7: 45,635,318 (GRCm38)	Y703C	possibly damaging	Het
Recql	T	A	6: 142,364,598 (GRCm38)	Q502L	probably benign	Het
Rgs9	A	T	11: 109,239,499 (GRCm38)	Y383*	probably null	Het
Rimbp3	T	C	16: 17,212,632 (GRCm38)	S1307P	probably benign	Het
Rspo1	A	G	4: 125,007,745 (GRCm38)	T200A	probably damaging	Het
Ryr3	C	T	2: 112,867,292 (GRCm38)	M922I	probably damaging	Het
Samm50	C	T	15: 84,211,127 (GRCm38)	A438V	probably damaging	Het
Sec1	A	C	7: 45,678,832 (GRCm38)	S264A	probably benign	Het
Sec31a	A	T	5: 100,381,336 (GRCm38)		probably null	Het
Slc13a3	T	C	2: 165,406,699 (GRCm38)	N553S	unknown	Het
Smg6	T	A	11: 74,946,116 (GRCm38)	L852Q	probably damaging	Het
Spata13	A	G	14: 60,691,725 (GRCm38)	N244S	probably damaging	Het
Srbd1	T	C	17: 86,057,685 (GRCm38)	R648G	probably damaging	Het
Sugct	T	G	13: 17,452,454 (GRCm38)		probably null	Het
Tmed10	A	G	12: 85,354,879 (GRCm38)	Y85H	probably damaging	Het
Trim68	A	G	7: 102,684,073 (GRCm38)	I134T	possibly damaging	Het
Tspyl3	G	A	2: 153,225,256 (GRCm38)	R21W	probably damaging	Het
Ttn	T	C	2: 76,810,699 (GRCm38)	I11863V	probably null	Het
Ubp1	G	A	9: 113,964,579 (GRCm38)	A283T	possibly damaging	Het
Ubtf	A	T	11: 102,314,918 (GRCm38)	F60L	probably damaging	Het
Vmn1r235	T	C	17: 21,261,737 (GRCm38)	I108T	probably benign	Het
Vmn2r125	T	C	4: 156,351,373 (GRCm38)	S349P	probably damaging	Het
Vmn2r25	A	G	6: 123,828,465 (GRCm38)	S478P	probably damaging	Het
Vmn2r88	T	A	14: 51,418,572 (GRCm38)	V746D	probably damaging	Het
Zcwpw1	A	G	5: 137,796,652 (GRCm38)	K37E	probably damaging	Het
Zdhhc24	T	C	19: 4,883,766 (GRCm38)	S284P	probably damaging	Het
Zswim8	C	A	14: 20,716,327 (GRCm38)	H841N	probably damaging	Het

Other mutations in Trio

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Trio	APN	15	27,912,743 (GRCm38)	splice site	probably benign
IGL01011:Trio	APN	15	27,736,489 (GRCm38)	missense	probably damaging	0.96
IGL01090:Trio	APN	15	27,773,007 (GRCm38)	missense	probably damaging	1.00
IGL01145:Trio	APN	15	27,818,167 (GRCm38)	splice site	probably benign
IGL01147:Trio	APN	15	27,881,320 (GRCm38)	missense	probably damaging	1.00
IGL01161:Trio	APN	15	27,749,781 (GRCm38)	missense	probably damaging	1.00
IGL01324:Trio	APN	15	27,905,323 (GRCm38)	missense	probably benign	0.42
IGL01352:Trio	APN	15	27,901,229 (GRCm38)	missense	probably benign	0.01
IGL01366:Trio	APN	15	27,732,868 (GRCm38)	missense	possibly damaging	0.76
IGL01443:Trio	APN	15	27,838,775 (GRCm38)	splice site	probably benign
IGL01454:Trio	APN	15	27,832,985 (GRCm38)	missense	probably benign	0.32
IGL01695:Trio	APN	15	27,773,001 (GRCm38)	missense	probably damaging	1.00
IGL01765:Trio	APN	15	27,764,026 (GRCm38)	missense	possibly damaging	0.85
IGL01860:Trio	APN	15	27,846,810 (GRCm38)	missense	probably damaging	1.00
IGL01879:Trio	APN	15	27,741,033 (GRCm38)	missense	probably benign	0.12
IGL01991:Trio	APN	15	27,871,274 (GRCm38)	missense	possibly damaging	0.95
IGL02106:Trio	APN	15	27,744,158 (GRCm38)	missense	possibly damaging	0.85
IGL02209:Trio	APN	15	27,744,053 (GRCm38)	missense	probably damaging	1.00
IGL02232:Trio	APN	15	27,902,561 (GRCm38)	missense	probably benign	0.24
IGL02304:Trio	APN	15	27,735,436 (GRCm38)	missense	probably damaging	0.96
IGL02504:Trio	APN	15	27,847,390 (GRCm38)	nonsense	probably null
IGL02508:Trio	APN	15	27,818,104 (GRCm38)	missense	possibly damaging	0.65
IGL02541:Trio	APN	15	27,844,930 (GRCm38)	splice site	probably benign
IGL02617:Trio	APN	15	27,841,849 (GRCm38)	splice site	probably benign
IGL02675:Trio	APN	15	27,768,039 (GRCm38)	unclassified	probably benign
IGL02817:Trio	APN	15	27,902,881 (GRCm38)	missense	probably benign	0.01
IGL02993:Trio	APN	15	27,830,239 (GRCm38)	splice site	probably benign
IGL03007:Trio	APN	15	27,902,742 (GRCm38)	missense	probably damaging	0.99
IGL03135:Trio	APN	15	27,832,011 (GRCm38)	splice site	probably benign
IGL03225:Trio	APN	15	27,902,695 (GRCm38)	missense	probably benign	0.30
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0302:Trio	UTSW	15	27,902,517 (GRCm38)	missense	probably damaging	1.00
R0505:Trio	UTSW	15	27,767,907 (GRCm38)	missense	probably benign	0.00
R0506:Trio	UTSW	15	27,854,963 (GRCm38)	missense	probably benign	0.12
R0564:Trio	UTSW	15	27,805,822 (GRCm38)	missense	probably damaging	1.00
R0659:Trio	UTSW	15	27,831,399 (GRCm38)	missense	probably damaging	0.97
R0882:Trio	UTSW	15	27,732,894 (GRCm38)	missense	probably damaging	1.00
R0939:Trio	UTSW	15	27,741,250 (GRCm38)	critical splice donor site	probably null
R1018:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R1439:Trio	UTSW	15	27,897,914 (GRCm38)	missense	probably damaging	1.00
R1456:Trio	UTSW	15	27,753,804 (GRCm38)	splice site	probably benign
R1488:Trio	UTSW	15	27,740,967 (GRCm38)	missense	probably damaging	1.00
R1522:Trio	UTSW	15	27,732,640 (GRCm38)	missense	probably benign	0.28
R1531:Trio	UTSW	15	27,832,985 (GRCm38)	missense	probably benign	0.32
R1640:Trio	UTSW	15	27,833,044 (GRCm38)	missense	probably damaging	1.00
R1646:Trio	UTSW	15	27,758,347 (GRCm38)	missense	possibly damaging	0.91
R1682:Trio	UTSW	15	27,744,146 (GRCm38)	splice site	probably null
R1791:Trio	UTSW	15	27,841,756 (GRCm38)	missense	probably damaging	1.00
R1803:Trio	UTSW	15	27,748,340 (GRCm38)	missense	probably benign
R1817:Trio	UTSW	15	27,742,495 (GRCm38)	nonsense	probably null
R1853:Trio	UTSW	15	27,756,536 (GRCm38)	missense	probably damaging	1.00
R1898:Trio	UTSW	15	27,742,380 (GRCm38)	missense	possibly damaging	0.52
R1937:Trio	UTSW	15	27,833,056 (GRCm38)	missense	probably damaging	1.00
R1938:Trio	UTSW	15	27,732,891 (GRCm38)	missense	probably damaging	0.98
R2025:Trio	UTSW	15	27,773,927 (GRCm38)	missense	probably damaging	1.00
R2025:Trio	UTSW	15	27,744,137 (GRCm38)	missense	probably damaging	0.99
R2050:Trio	UTSW	15	27,851,945 (GRCm38)	missense	possibly damaging	0.85
R2186:Trio	UTSW	15	27,823,975 (GRCm38)	splice site	probably null
R2913:Trio	UTSW	15	27,854,912 (GRCm38)	missense	probably damaging	1.00
R3151:Trio	UTSW	15	27,805,776 (GRCm38)	missense	probably damaging	1.00
R3771:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3773:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3826:Trio	UTSW	15	27,833,070 (GRCm38)	missense	probably damaging	1.00
R4015:Trio	UTSW	15	27,744,101 (GRCm38)	missense	possibly damaging	0.71
R4359:Trio	UTSW	15	27,749,797 (GRCm38)	nonsense	probably null
R4370:Trio	UTSW	15	27,748,337 (GRCm38)	nonsense	probably null
R4547:Trio	UTSW	15	27,818,982 (GRCm38)	missense	possibly damaging	0.89
R4573:Trio	UTSW	15	27,772,998 (GRCm38)	small deletion	probably benign
R4620:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R4735:Trio	UTSW	15	27,752,789 (GRCm38)	splice site	probably null
R4764:Trio	UTSW	15	27,732,538 (GRCm38)	nonsense	probably null
R4775:Trio	UTSW	15	27,881,342 (GRCm38)	nonsense	probably null
R4942:Trio	UTSW	15	27,752,725 (GRCm38)	missense	probably benign	0.21
R5004:Trio	UTSW	15	27,755,178 (GRCm38)	missense	probably damaging	1.00
R5149:Trio	UTSW	15	27,754,029 (GRCm38)	missense	possibly damaging	0.74
R5183:Trio	UTSW	15	27,902,600 (GRCm38)	missense	probably benign	0.00
R5186:Trio	UTSW	15	27,897,991 (GRCm38)	missense	probably damaging	0.97
R5268:Trio	UTSW	15	27,748,286 (GRCm38)	missense	probably benign	0.02
R5344:Trio	UTSW	15	27,735,532 (GRCm38)	missense	probably benign	0.12
R5407:Trio	UTSW	15	27,844,806 (GRCm38)	splice site	probably null
R5442:Trio	UTSW	15	27,856,194 (GRCm38)	missense	probably benign	0.04
R5617:Trio	UTSW	15	27,902,748 (GRCm38)	missense	probably benign
R5778:Trio	UTSW	15	27,856,164 (GRCm38)	missense	probably benign	0.33
R5986:Trio	UTSW	15	27,851,933 (GRCm38)	missense	possibly damaging	0.88
R5990:Trio	UTSW	15	27,891,459 (GRCm38)	missense	probably benign	0.10
R6011:Trio	UTSW	15	27,735,545 (GRCm38)	missense	probably damaging	0.98
R6063:Trio	UTSW	15	27,891,379 (GRCm38)	missense	possibly damaging	0.94
R6166:Trio	UTSW	15	27,818,071 (GRCm38)	missense	probably damaging	0.96
R6187:Trio	UTSW	15	27,743,952 (GRCm38)	critical splice donor site	probably null
R6387:Trio	UTSW	15	27,752,739 (GRCm38)	missense	probably damaging	1.00
R6402:Trio	UTSW	15	27,902,911 (GRCm38)	missense	probably benign	0.02
R6478:Trio	UTSW	15	27,856,107 (GRCm38)	missense	probably benign	0.01
R6528:Trio	UTSW	15	27,805,870 (GRCm38)	missense	probably damaging	1.00
R6662:Trio	UTSW	15	27,854,996 (GRCm38)	missense	probably benign	0.00
R6825:Trio	UTSW	15	27,889,308 (GRCm38)	missense	probably damaging	0.98
R6890:Trio	UTSW	15	27,919,288 (GRCm38)	unclassified	probably benign
R6945:Trio	UTSW	15	27,824,090 (GRCm38)	missense	probably damaging	1.00
R7027:Trio	UTSW	15	27,805,654 (GRCm38)	missense	possibly damaging	0.86
R7046:Trio	UTSW	15	27,832,051 (GRCm38)	missense	probably damaging	1.00
R7049:Trio	UTSW	15	27,749,799 (GRCm38)	missense	possibly damaging	0.66
R7075:Trio	UTSW	15	27,898,000 (GRCm38)	missense	unknown
R7094:Trio	UTSW	15	27,891,448 (GRCm38)	missense	unknown
R7123:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7130:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7214:Trio	UTSW	15	27,871,187 (GRCm38)	missense	probably damaging	0.97
R7292:Trio	UTSW	15	27,828,351 (GRCm38)	missense	possibly damaging	0.63
R7293:Trio	UTSW	15	27,871,289 (GRCm38)	missense	possibly damaging	0.66
R7352:Trio	UTSW	15	27,732,876 (GRCm38)	missense	probably damaging	0.96
R7426:Trio	UTSW	15	27,856,107 (GRCm38)	missense	probably benign	0.01
R7451:Trio	UTSW	15	27,747,913 (GRCm38)	missense	probably benign	0.07
R7558:Trio	UTSW	15	27,831,394 (GRCm38)	missense	possibly damaging	0.90
R7578:Trio	UTSW	15	27,854,939 (GRCm38)	missense	possibly damaging	0.94
R7596:Trio	UTSW	15	27,749,826 (GRCm38)	missense	probably damaging	0.99
R7604:Trio	UTSW	15	27,736,445 (GRCm38)	critical splice donor site	probably null
R7609:Trio	UTSW	15	27,912,642 (GRCm38)	missense	unknown
R7767:Trio	UTSW	15	27,889,418 (GRCm38)	missense	unknown
R7784:Trio	UTSW	15	27,763,994 (GRCm38)	missense	probably damaging	1.00
R7817:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R7833:Trio	UTSW	15	27,774,086 (GRCm38)	missense	probably damaging	0.99
R7873:Trio	UTSW	15	27,805,684 (GRCm38)	missense	possibly damaging	0.83
R7879:Trio	UTSW	15	27,851,924 (GRCm38)	missense	possibly damaging	0.94
R7989:Trio	UTSW	15	27,772,935 (GRCm38)	missense	probably damaging	0.97
R8022:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R8050:Trio	UTSW	15	27,891,454 (GRCm38)	missense	unknown
R8217:Trio	UTSW	15	27,818,969 (GRCm38)	missense	probably damaging	0.97
R8280:Trio	UTSW	15	27,902,910 (GRCm38)	missense	unknown
R8283:Trio	UTSW	15	27,756,542 (GRCm38)	missense	possibly damaging	0.79
R8300:Trio	UTSW	15	27,855,022 (GRCm38)	missense	possibly damaging	0.66
R8321:Trio	UTSW	15	27,881,326 (GRCm38)	missense	possibly damaging	0.90
R8477:Trio	UTSW	15	27,773,952 (GRCm38)	missense	possibly damaging	0.83
R8479:Trio	UTSW	15	27,901,200 (GRCm38)	missense	probably benign	0.25
R8682:Trio	UTSW	15	27,905,192 (GRCm38)	missense	unknown
R8688:Trio	UTSW	15	27,748,238 (GRCm38)	missense	possibly damaging	0.61
R8708:Trio	UTSW	15	27,732,546 (GRCm38)	missense	probably damaging	0.99
R8709:Trio	UTSW	15	27,919,237 (GRCm38)	missense	unknown
R8713:Trio	UTSW	15	27,743,951 (GRCm38)	critical splice donor site	probably benign
R8798:Trio	UTSW	15	27,851,837 (GRCm38)	missense	possibly damaging	0.92
R8812:Trio	UTSW	15	27,905,225 (GRCm38)	missense	unknown
R8816:Trio	UTSW	15	27,741,271 (GRCm38)	missense	probably damaging	0.96
R8828:Trio	UTSW	15	27,741,064 (GRCm38)	missense	possibly damaging	0.93
R8987:Trio	UTSW	15	27,732,687 (GRCm38)	missense	probably benign	0.23
R9051:Trio	UTSW	15	27,732,684 (GRCm38)	missense	possibly damaging	0.78
R9069:Trio	UTSW	15	27,852,011 (GRCm38)	missense	possibly damaging	0.83
R9075:Trio	UTSW	15	27,773,936 (GRCm38)	nonsense	probably null
R9079:Trio	UTSW	15	27,732,937 (GRCm38)	missense	possibly damaging	0.52
R9139:Trio	UTSW	15	27,749,836 (GRCm38)	nonsense	probably null
R9494:Trio	UTSW	15	27,846,757 (GRCm38)	missense	probably benign	0.00
R9680:Trio	UTSW	15	27,744,072 (GRCm38)	missense	possibly damaging	0.93
R9720:Trio	UTSW	15	27,847,409 (GRCm38)	missense	probably benign	0.00
R9726:Trio	UTSW	15	27,912,666 (GRCm38)	missense	unknown
X0024:Trio	UTSW	15	27,765,726 (GRCm38)	missense	possibly damaging	0.91
Z1176:Trio	UTSW	15	27,771,387 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAGCCTGTGAATGACGATCCTGTG -3'
(R):5'- AGGCAGCAGCAGTAGCAACATC -3'

Sequencing Primer
(F):5'- AATGACGATCCTGTGTCTCTCAAG -3'
(R):5'- ATCTCCACCATGCTGGTGAC -3'

Posted On

2014-05-23