Mutagenetix > Incidental Mutation

Incidental Mutation 'R5802:Stk10'

501565

Institutional Source

Beutler Lab

Gene Symbol

Stk10

Ensembl Gene

ENSMUSG00000020272

Gene Name

serine/threonine kinase 10

Synonyms

Gek1, Lok

MMRRC Submission

043391-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

R5802 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32533305-32624587 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 32596748 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 335 (P335L)

Ref Sequence

ENSEMBL: ENSMUSP00000099885 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102821]

AlphaFold

O55098

Predicted Effect

probably benign
Transcript: ENSMUST00000102821
AA Change: P335L

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000099885
Gene: ENSMUSG00000020272
AA Change: P335L

Domain	Start	End	E-Value	Type
S_TKc	36	294	8.66e-92	SMART
low complexity region	316	334	N/A	INTRINSIC
low complexity region	544	577	N/A	INTRINSIC
Pfam:PKK	586	724	1.9e-41	PFAM
Pfam:PKK	754	894	2.2e-37	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143397

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

97% (63/65)

MGI Phenotype

FUNCTION: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Targeted disruption of this gene results in enhanced cell adhesion in mitogen-stimulated T cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	A	T	1: 11,950,964	D383V	probably damaging	Het
Abca4	A	T	3: 122,054,232	L67F	probably damaging	Het
Abcc9	C	A	6: 142,656,676		probably null	Het
Atp2a3	T	C	11: 72,972,882	V175A	probably damaging	Het
B3galt4	T	A	17: 33,950,757	D169V	probably damaging	Het
Bsn	C	T	9: 108,113,009	R1848Q	possibly damaging	Het
Camkk2	T	C	5: 122,734,244	E90G	probably damaging	Het
Cdon	T	A	9: 35,454,420	I155N	probably damaging	Het
Cep70	A	G	9: 99,296,405	N519D	probably damaging	Het
Clgn	T	C	8: 83,425,614	S582P	probably damaging	Het
Cnga3	T	C	1: 37,260,925	F280S	probably damaging	Het
Dennd4c	C	T	4: 86,811,453	T764M	probably benign	Het
Dis3	A	T	14: 99,099,664	S4T	probably damaging	Het
Dlgap1	T	C	17: 70,766,091		probably null	Het
Dnah17	C	T	11: 118,036,446	V3839I	possibly damaging	Het
Dnajc1	A	G	2: 18,284,739	Y286H	probably benign	Het
Dynap	T	A	18: 70,241,002	D151V	unknown	Het
Ednrb	A	G	14: 103,821,714	F292S	probably damaging	Het
Eef1a1	A	G	9: 78,479,036	S396P	probably damaging	Het
Fancg	A	G	4: 43,006,582	F324S	probably benign	Het
Fbxw11	T	A	11: 32,711,790	S56T	probably benign	Het
Gm13178	T	A	4: 144,703,636	D261V	probably damaging	Het
Gm14409	T	C	2: 177,265,256	T150A	possibly damaging	Het
Gm17535	G	C	9: 3,035,758	V209L	probably benign	Homo
Gm20767	G	A	13: 120,154,913	S96N	possibly damaging	Het
Gm5592	C	T	7: 41,219,105		probably benign	Het
Gm7030	A	G	17: 36,111,287		probably benign	Het
Gpr137	C	T	19: 6,942,005	W51*	probably null	Het
Hbb-bs	T	C	7: 103,826,672	Y146C	probably damaging	Het
Herc1	G	T	9: 66,462,878	C2982F	probably damaging	Het
Hnrnpa3	T	A	2: 75,665,056	N309K	unknown	Het
Hydin	A	T	8: 110,452,060	I1096F	possibly damaging	Het
Klf12	A	G	14: 100,022,894	V133A	probably benign	Het
Lats2	A	T	14: 57,694,418	Y848N	probably damaging	Het
Loxl3	A	G	6: 83,049,289	T453A	possibly damaging	Het
Ltn1	T	G	16: 87,415,681	H664P	probably benign	Het
Lypd6	C	T	2: 50,173,601	T40I	probably benign	Het
Nbeal1	A	T	1: 60,272,221	T1817S	probably benign	Het
Nub1	A	C	5: 24,702,441	Y350S	possibly damaging	Het
Pbp2	A	G	6: 135,309,876	Y158H	possibly damaging	Het
Ptpru	C	T	4: 131,788,377	E827K	possibly damaging	Het
Rap1a	A	G	3: 105,745,936	Y32H	probably damaging	Het
Raph1	T	C	1: 60,488,673	N1143S	possibly damaging	Het
Rbl1	T	C	2: 157,161,433	T859A	probably benign	Het
Rom1	A	G	19: 8,928,824	L117P	probably damaging	Het
Senp6	T	C	9: 80,118,644		probably benign	Het
Sirpb1c	T	C	3: 15,832,076	M379V	probably benign	Het
Slc6a4	C	T	11: 77,019,236	T439M	probably damaging	Het
Srsf12	G	T	4: 33,230,929	R141L	probably damaging	Het
Tecpr1	A	T	5: 144,206,546	N670K	probably benign	Het
Tgds	T	C	14: 118,132,707	E8G	probably benign	Het
Tmem129	A	G	5: 33,657,716	S38P	probably damaging	Het
Trappc9	T	C	15: 72,685,339	E812G	probably damaging	Het
Trmt10b	T	A	4: 45,314,236		probably benign	Het
Wapl	A	G	14: 34,692,320	T380A	probably damaging	Het
Xylt1	A	T	7: 117,656,691	T829S	probably benign	Het
Zc3h6	C	T	2: 129,015,559	P666L	possibly damaging	Het
Zfp703	C	T	8: 26,979,205	P299L	probably damaging	Het

Other mutations in Stk10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01105:Stk10	APN	11	32577740	missense	probably benign	0.33
IGL01285:Stk10	APN	11	32610653	missense	possibly damaging	0.91
IGL01983:Stk10	APN	11	32589460	missense	probably benign	0.05
IGL03177:Stk10	APN	11	32614592	missense	probably damaging	1.00
IGL03183:Stk10	APN	11	32604143	missense	possibly damaging	0.50
coquet	UTSW	11	32577764	missense
legacy	UTSW	11	32604166	nonsense	probably null
mignon	UTSW	11	32587363	missense	probably damaging	1.00
R0481_stk10_383	UTSW	11	32614708	missense	probably damaging	1.00
FR4976:Stk10	UTSW	11	32614520	critical splice acceptor site	probably benign
R0003:Stk10	UTSW	11	32589460	missense	probably benign	0.05
R0008:Stk10	UTSW	11	32587305	splice site	probably benign
R0056:Stk10	UTSW	11	32617851	missense	possibly damaging	0.95
R0076:Stk10	UTSW	11	32603722	missense	probably benign
R0227:Stk10	UTSW	11	32617859	missense	probably damaging	1.00
R0440:Stk10	UTSW	11	32604190	missense	probably damaging	1.00
R0454:Stk10	UTSW	11	32596724	missense	probably damaging	0.99
R0481:Stk10	UTSW	11	32614708	missense	probably damaging	1.00
R0504:Stk10	UTSW	11	32617882	missense	probably benign	0.04
R0790:Stk10	UTSW	11	32598653	missense	probably benign	0.00
R1439:Stk10	UTSW	11	32617919	missense	probably damaging	0.98
R1539:Stk10	UTSW	11	32533440	missense	possibly damaging	0.85
R1770:Stk10	UTSW	11	32622464	missense	possibly damaging	0.94
R4304:Stk10	UTSW	11	32610634	missense	probably damaging	0.97
R4430:Stk10	UTSW	11	32533552	missense	possibly damaging	0.81
R4702:Stk10	UTSW	11	32555172	missense	probably benign	0.28
R4797:Stk10	UTSW	11	32598471	missense	probably benign	0.01
R5447:Stk10	UTSW	11	32604166	nonsense	probably null
R5801:Stk10	UTSW	11	32596748	missense	probably benign	0.01
R6129:Stk10	UTSW	11	32615871	missense	probably damaging	1.00
R6154:Stk10	UTSW	11	32603654	splice site	probably null
R6175:Stk10	UTSW	11	32603761	missense	possibly damaging	0.46
R6185:Stk10	UTSW	11	32577749	missense	probably benign	0.13
R6520:Stk10	UTSW	11	32588839	missense	probably damaging	1.00
R6824:Stk10	UTSW	11	32587363	missense	probably damaging	1.00
R7259:Stk10	UTSW	11	32598497	missense	probably benign	0.00
R7649:Stk10	UTSW	11	32577764	missense
R8331:Stk10	UTSW	11	32588928	missense
R8847:Stk10	UTSW	11	32589427	missense
R9252:Stk10	UTSW	11	32588915	missense
R9367:Stk10	UTSW	11	32588878	missense
X0027:Stk10	UTSW	11	32587361	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCAGAGTTGTTCCTCGTTGC -3'
(R):5'- GCATGCTCCAGAACTTAGACC -3'

Sequencing Primer
(F):5'- TCGTTGCTTCAGGACCAAG -3'
(R):5'- AACTTAGACCTGGCCAGTTG -3'

Posted On

2017-12-01