Incidental Mutation 'R6762:Nos2'
| ID |
531754 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nos2
|
| Ensembl Gene |
ENSMUSG00000020826 |
| Gene Name |
nitric oxide synthase 2, inducible |
| Synonyms |
iNOS, Nos-2, Nos2a, NOS-II |
| MMRRC Submission |
044878-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R6762 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
11 |
| Chromosomal Location |
78920787-78960254 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to G
at 78959748 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Arginine
at position 1144
(L1144R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000018610
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000018610]
[ENSMUST00000073001]
[ENSMUST00000108268]
[ENSMUST00000108269]
[ENSMUST00000214397]
|
| AlphaFold |
P29477 |
| PDB Structure |
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) (N-[(1,3-BENZODIOXOL-5-YL)METHYL]-1-[2-(1H-IMIDAZOL-1-YL)PYRIMIDIN-4-YL]-4-(METHOXYCARBONYL)-PIPERAZINE-2-ACETAMIDE COMPLEX [X-RAY DIFFRACTION]
MURINE INOSOXY DIMER WITH ISOTHIOUREA BOUND IN THE ACTIVE SITE [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND 4-AMINO TETRAHYDROBIOPTERIN [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND DIHYDROBIOPTERIN [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND TETRAHYDROBIOPTERIN [X-RAY DIFFRACTION]
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site [X-RAY DIFFRACTION]
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457A Mutation at Tetrahydrobiopterin Binding Site [X-RAY DIFFRACTION]
inducible nitric oxide synthase with Chlorzoxazone bound [X-RAY DIFFRACTION]
inducible nitric oxide synthase with 7-nitroindazole bound [X-RAY DIFFRACTION]
inducible nitric oxide synthase with 6-nitroindazole bound [X-RAY DIFFRACTION]
>> 40 additional structures at PDB <<
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000018610
AA Change: L1144R
PolyPhen 2
Score 0.905 (Sensitivity: 0.82; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000018610
Gene: ENSMUSG00000020826
AA Change: L1144R
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:NO_synthase
|
129 |
491 |
6.7e-189 |
PFAM |
|
Pfam:Flavodoxin_1
|
535 |
666 |
5.5e-43 |
PFAM |
|
Pfam:FAD_binding_1
|
719 |
941 |
8.8e-79 |
PFAM |
|
Pfam:NAD_binding_1
|
973 |
1087 |
4.1e-24 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000073001
|
| SMART Domains |
Protein: ENSMUSP00000072764
Gene: ENSMUSG00000001123
| Domain | Start | End | E-Value | Type |
|---|
|
GLECT
|
15 |
147 |
6.58e-55 |
SMART |
|
Gal-bind_lectin
|
21 |
146 |
5.24e-55 |
SMART |
|
GLECT
|
222 |
352 |
5.38e-60 |
SMART |
|
Gal-bind_lectin
|
228 |
352 |
1.33e-65 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108268
|
| SMART Domains |
Protein: ENSMUSP00000103903
Gene: ENSMUSG00000001123
| Domain | Start | End | E-Value | Type |
|---|
|
GLECT
|
15 |
147 |
6.58e-55 |
SMART |
|
Gal-bind_lectin
|
21 |
146 |
5.24e-55 |
SMART |
|
GLECT
|
192 |
322 |
5.38e-60 |
SMART |
|
Gal-bind_lectin
|
198 |
322 |
1.33e-65 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108269
|
| SMART Domains |
Protein: ENSMUSP00000103904
Gene: ENSMUSG00000001123
| Domain | Start | End | E-Value | Type |
|---|
|
GLECT
|
15 |
147 |
6.58e-55 |
SMART |
|
Gal-bind_lectin
|
21 |
146 |
5.24e-55 |
SMART |
|
GLECT
|
223 |
353 |
5.38e-60 |
SMART |
|
Gal-bind_lectin
|
229 |
353 |
1.33e-65 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000214397
AA Change: L1031R
PolyPhen 2
Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)
|
| Meta Mutation Damage Score |
0.1070 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.7%
- 10x: 98.4%
- 20x: 95.7%
|
| Validation Efficiency |
100% (45/45) |
| MGI Phenotype |
FUNCTION: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygous deletion of this gene alters susceptibility to infection, response to injury, sepsis and sensory stimuli, cardiac function, osteoclast, platelet and mammary gland physiology, tumor growth, testis and uterus morphology, diet-induced atherosclerosis, and blood, urine and glucose metabolism. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 45 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Ackr4 |
A |
G |
9: 104,099,668 (GRCm38) |
Y27H |
probably benign |
Het |
| Angptl3 |
T |
A |
4: 99,037,417 (GRCm38) |
S327T |
possibly damaging |
Het |
| Arl6ip4 |
GGAAGAAGAAGAAGAAGAA |
GGAAGAAGAAGAAGAAGAAGAA |
5: 124,117,050 (GRCm38) |
|
probably benign |
Het |
| Cyp2s1 |
A |
T |
7: 25,808,070 (GRCm38) |
L318H |
probably damaging |
Het |
| Dnah14 |
T |
C |
1: 181,757,259 (GRCm38) |
L3185P |
probably damaging |
Het |
| Dnah7b |
T |
C |
1: 46,224,742 (GRCm38) |
V2128A |
probably benign |
Het |
| Ehhadh |
A |
T |
16: 21,762,459 (GRCm38) |
F594L |
probably benign |
Het |
| En2 |
A |
G |
5: 28,170,353 (GRCm38) |
N298S |
possibly damaging |
Het |
| Epha5 |
T |
A |
5: 84,331,726 (GRCm38) |
N140Y |
probably damaging |
Het |
| Fam151b |
C |
T |
13: 92,468,050 (GRCm38) |
V144I |
possibly damaging |
Het |
| Fanca |
C |
A |
8: 123,271,303 (GRCm38) |
A1215S |
probably benign |
Het |
| Fancd2 |
T |
A |
6: 113,586,016 (GRCm38) |
|
probably null |
Het |
| Fat2 |
T |
C |
11: 55,253,482 (GRCm38) |
|
probably null |
Het |
| Gm4513 |
T |
C |
7: 20,594,193 (GRCm38) |
N31S |
probably benign |
Het |
| Hspg2 |
A |
T |
4: 137,551,803 (GRCm38) |
I3066F |
possibly damaging |
Het |
| Krt10 |
T |
C |
11: 99,387,057 (GRCm38) |
T355A |
possibly damaging |
Het |
| Lims1 |
C |
A |
10: 58,412,545 (GRCm38) |
H275N |
probably damaging |
Het |
| Map3k19 |
C |
T |
1: 127,847,264 (GRCm38) |
G112D |
probably damaging |
Het |
| Mdn1 |
T |
A |
4: 32,676,786 (GRCm38) |
N619K |
possibly damaging |
Het |
| Mpp6 |
T |
A |
6: 50,180,438 (GRCm38) |
|
probably null |
Het |
| Mtor |
A |
G |
4: 148,538,481 (GRCm38) |
T1977A |
possibly damaging |
Het |
| Olfr1085 |
A |
T |
2: 86,657,844 (GRCm38) |
F205I |
probably benign |
Het |
| Olfr608 |
G |
C |
7: 103,470,389 (GRCm38) |
V117L |
probably benign |
Het |
| Olfr780 |
G |
A |
10: 129,322,256 (GRCm38) |
C211Y |
probably damaging |
Het |
| Pcdhga9 |
C |
A |
18: 37,737,268 (GRCm38) |
S50Y |
probably damaging |
Het |
| Pfn4 |
T |
A |
12: 4,775,487 (GRCm38) |
M108K |
probably damaging |
Het |
| Prep |
T |
C |
10: 45,148,123 (GRCm38) |
|
probably null |
Het |
| Qtrt1 |
T |
A |
9: 21,412,082 (GRCm38) |
H76Q |
probably damaging |
Het |
| Rpa1 |
T |
A |
11: 75,340,345 (GRCm38) |
S73C |
possibly damaging |
Het |
| Senp5 |
A |
G |
16: 31,989,884 (GRCm38) |
V157A |
probably damaging |
Het |
| Snapc3 |
T |
C |
4: 83,435,258 (GRCm38) |
L178P |
probably damaging |
Het |
| Sptbn4 |
A |
G |
7: 27,394,208 (GRCm38) |
F1340L |
probably damaging |
Het |
| Srd5a3 |
T |
A |
5: 76,153,551 (GRCm38) |
I85K |
probably benign |
Het |
| Taar2 |
C |
T |
10: 23,941,402 (GRCm38) |
T280M |
probably damaging |
Het |
| Tgfb3 |
T |
C |
12: 86,069,463 (GRCm38) |
D177G |
probably benign |
Het |
| Tpt1 |
A |
G |
14: 75,846,381 (GRCm38) |
D94G |
probably benign |
Het |
| Trpm4 |
A |
G |
7: 45,304,816 (GRCm38) |
|
probably benign |
Het |
| Trpv4 |
C |
T |
5: 114,625,110 (GRCm38) |
R746H |
probably benign |
Het |
| Txndc2 |
T |
C |
17: 65,638,972 (GRCm38) |
D70G |
probably damaging |
Het |
| Ugcg |
T |
A |
4: 59,219,530 (GRCm38) |
I289N |
possibly damaging |
Het |
| Vmn2r2 |
C |
G |
3: 64,134,449 (GRCm38) |
D282H |
probably damaging |
Het |
| Vmn2r50 |
A |
T |
7: 10,053,083 (GRCm38) |
N32K |
probably benign |
Het |
| Wdpcp |
C |
T |
11: 21,721,244 (GRCm38) |
T495I |
probably benign |
Het |
| Zfp961 |
T |
A |
8: 71,966,114 (GRCm38) |
C51S |
possibly damaging |
Het |
| Zxdc |
C |
T |
6: 90,382,183 (GRCm38) |
A599V |
probably benign |
Het |
|
| Other mutations in Nos2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01388:Nos2
|
APN |
11 |
78,957,452 (GRCm38) |
missense |
probably damaging |
0.96 |
|
IGL01503:Nos2
|
APN |
11 |
78,945,863 (GRCm38) |
splice site |
probably benign |
|
|
IGL01789:Nos2
|
APN |
11 |
78,944,657 (GRCm38) |
splice site |
probably benign |
|
|
IGL02797:Nos2
|
APN |
11 |
78,940,344 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02968:Nos2
|
APN |
11 |
78,937,637 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6762_Nos2_754
|
UTSW |
11 |
78,959,748 (GRCm38) |
missense |
possibly damaging |
0.90 |
|
R0035:Nos2
|
UTSW |
11 |
78,945,727 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0265:Nos2
|
UTSW |
11 |
78,937,602 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R0441:Nos2
|
UTSW |
11 |
78,928,583 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0504:Nos2
|
UTSW |
11 |
78,940,077 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0570:Nos2
|
UTSW |
11 |
78,935,361 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R1356:Nos2
|
UTSW |
11 |
78,952,803 (GRCm38) |
missense |
probably benign |
0.00 |
|
R1538:Nos2
|
UTSW |
11 |
78,956,570 (GRCm38) |
missense |
probably benign |
0.00 |
|
R3414:Nos2
|
UTSW |
11 |
78,957,588 (GRCm38) |
missense |
probably benign |
0.14 |
|
R3418:Nos2
|
UTSW |
11 |
78,959,695 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4279:Nos2
|
UTSW |
11 |
78,929,776 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4492:Nos2
|
UTSW |
11 |
78,950,095 (GRCm38) |
missense |
probably benign |
|
|
R4632:Nos2
|
UTSW |
11 |
78,957,591 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R4686:Nos2
|
UTSW |
11 |
78,928,630 (GRCm38) |
missense |
possibly damaging |
0.65 |
|
R5038:Nos2
|
UTSW |
11 |
78,922,314 (GRCm38) |
missense |
probably benign |
|
|
R5214:Nos2
|
UTSW |
11 |
78,955,441 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5377:Nos2
|
UTSW |
11 |
78,957,491 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5777:Nos2
|
UTSW |
11 |
78,940,152 (GRCm38) |
missense |
probably null |
1.00 |
|
R5834:Nos2
|
UTSW |
11 |
78,928,579 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5930:Nos2
|
UTSW |
11 |
78,937,915 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6511:Nos2
|
UTSW |
11 |
78,955,464 (GRCm38) |
splice site |
probably null |
|
|
R6706:Nos2
|
UTSW |
11 |
78,944,723 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R6747:Nos2
|
UTSW |
11 |
78,952,954 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R6817:Nos2
|
UTSW |
11 |
78,945,266 (GRCm38) |
missense |
possibly damaging |
0.64 |
|
R6868:Nos2
|
UTSW |
11 |
78,957,506 (GRCm38) |
missense |
probably benign |
0.02 |
|
R6917:Nos2
|
UTSW |
11 |
78,951,227 (GRCm38) |
missense |
possibly damaging |
0.50 |
|
R7082:Nos2
|
UTSW |
11 |
78,928,579 (GRCm38) |
missense |
probably benign |
0.02 |
|
R7286:Nos2
|
UTSW |
11 |
78,929,854 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7367:Nos2
|
UTSW |
11 |
78,950,090 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7398:Nos2
|
UTSW |
11 |
78,936,471 (GRCm38) |
nonsense |
probably null |
|
|
R7411:Nos2
|
UTSW |
11 |
78,944,855 (GRCm38) |
critical splice donor site |
probably null |
|
|
R7469:Nos2
|
UTSW |
11 |
78,952,971 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R7736:Nos2
|
UTSW |
11 |
78,922,366 (GRCm38) |
nonsense |
probably null |
|
|
R8694:Nos2
|
UTSW |
11 |
78,945,689 (GRCm38) |
missense |
possibly damaging |
0.93 |
|
R8832:Nos2
|
UTSW |
11 |
78,955,464 (GRCm38) |
splice site |
probably null |
|
|
R8872:Nos2
|
UTSW |
11 |
78,949,123 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R8952:Nos2
|
UTSW |
11 |
78,945,263 (GRCm38) |
missense |
probably benign |
0.00 |
|
R9433:Nos2
|
UTSW |
11 |
78,959,664 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9580:Nos2
|
UTSW |
11 |
78,937,631 (GRCm38) |
missense |
probably benign |
0.01 |
|
R9612:Nos2
|
UTSW |
11 |
78,949,158 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9727:Nos2
|
UTSW |
11 |
78,952,999 (GRCm38) |
missense |
possibly damaging |
0.51 |
|
R9747:Nos2
|
UTSW |
11 |
78,931,646 (GRCm38) |
missense |
probably damaging |
0.96 |
|
X0063:Nos2
|
UTSW |
11 |
78,922,367 (GRCm38) |
missense |
probably benign |
0.01 |
|
Z1177:Nos2
|
UTSW |
11 |
78,931,672 (GRCm38) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- GGGTTTTGTCCAAGCCTAAAC -3'
(R):5'- CAAAGTGCTTCAGTCAGGAGG -3'
Sequencing Primer
(F):5'- TGTCCAAGCCTAAACATGATAACTG -3'
(R):5'- CTCTCAGCTCCAAGGAAGAGTG -3'
|
| Posted On |
2018-08-29 |
Incidental Mutation