Mutagenetix > Incidental Mutation

Incidental Mutation 'R7183:Zfp87'

559127

Institutional Source

Beutler Lab

Gene Symbol

Zfp87

Ensembl Gene

ENSMUSG00000097333

Gene Name

zinc finger protein 87

Synonyms

Mzf22, 2210039O17Rik, Zfp72, KRAB4, 4631412O18Rik

MMRRC Submission

045235-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.098) question?

Stock #

R7183 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67515781-67526177 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 67517474 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 290 (S290P)

Ref Sequence

ENSEMBL: ENSMUSP00000138087 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167914] [ENSMUST00000180536] [ENSMUST00000181341] [ENSMUST00000181573] [ENSMUST00000181767]

AlphaFold

Q8K2A4

Predicted Effect

probably benign
Transcript: ENSMUST00000167914

SMART Domains

Protein: ENSMUSP00000137830
Gene: ENSMUSG00000097565

Domain	Start	End	E-Value	Type
KRAB	5	65	4.41e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180536

SMART Domains

Protein: ENSMUSP00000137812
Gene: ENSMUSG00000097333

Domain	Start	End	E-Value	Type
KRAB	5	51	7.02e-19	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181341

SMART Domains

Protein: ENSMUSP00000137689
Gene: ENSMUSG00000097333

Domain	Start	End	E-Value	Type
KRAB	5	65	4.41e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181573

SMART Domains

Protein: ENSMUSP00000138011
Gene: ENSMUSG00000097333

Domain	Start	End	E-Value	Type
KRAB	5	51	7.02e-19	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000181767
AA Change: S290P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000138087
Gene: ENSMUSG00000097333
AA Change: S290P

Domain	Start	End	E-Value	Type
KRAB	5	65	4.41e-34	SMART
ZnF_C2H2	109	131	2.61e-4	SMART
ZnF_C2H2	137	159	9.22e-5	SMART
ZnF_C2H2	165	187	2.36e-2	SMART
ZnF_C2H2	193	215	7.15e-2	SMART
ZnF_C2H2	221	243	9.22e-5	SMART
ZnF_C2H2	249	271	3.63e-3	SMART
ZnF_C2H2	277	299	6.42e-4	SMART
ZnF_C2H2	305	327	2.99e-4	SMART
ZnF_C2H2	333	355	2.4e-3	SMART
ZnF_C2H2	361	383	5.59e-4	SMART
ZnF_C2H2	389	411	3.69e-4	SMART
ZnF_C2H2	417	439	5.9e-3	SMART
ZnF_C2H2	445	467	3.21e-4	SMART
ZnF_C2H2	472	494	9.08e-4	SMART
ZnF_C2H2	500	522	4.47e-3	SMART

Meta Mutation Damage Score

0.6329

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (65/65)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931408C20Rik	G	A	1: 26,682,833	L1089F	probably benign	Het
Actn1	T	A	12: 80,168,932	M816L	possibly damaging	Het
Ahnak	T	C	19: 9,017,668	F5439L	probably damaging	Het
Apba2	G	A	7: 64,733,545	D369N	probably benign	Het
Arhgap32	A	G	9: 32,186,383	N228D	probably benign	Het
Arhgap33	T	A	7: 30,525,871		probably null	Het
Cacna1g	C	T	11: 94,439,737	C984Y	probably benign	Het
Cadm2	C	A	16: 66,882,832	G47*	probably null	Het
Ccdc125	A	G	13: 100,690,358	D241G	possibly damaging	Het
Ccdc39	T	G	3: 33,814,471	E822A	probably damaging	Het
Cd86	CA	CAA	16: 36,606,555		probably null	Het
Cdc42bpg	A	G	19: 6,310,797	D195G	probably damaging	Het
Cdkl1	T	C	12: 69,748,932	R275G	probably damaging	Het
Chst4	T	C	8: 110,029,998	N411S	possibly damaging	Het
Cir1	A	G	2: 73,286,386	V210A	probably damaging	Het
Col6a1	A	G	10: 76,716,259		probably null	Het
Crmp1	C	A	5: 37,288,817	H606N	probably benign	Het
Cyp2j8	A	T	4: 96,479,181	N233K	probably damaging	Het
Dennd1b	A	T	1: 139,170,252	Q677L	unknown	Het
Dnah17	G	A	11: 118,129,188	T11I	probably benign	Het
Ehd1	A	G	19: 6,297,654	H346R	probably benign	Het
Emc3	G	T	6: 113,531,384	Y33*	probably null	Het
Ercc5	A	T	1: 44,161,808		probably null	Het
Ercc5	G	T	1: 44,161,809		probably null	Het
Fat3	A	C	9: 15,922,837	I4153S	possibly damaging	Het
Fn3krp	T	C	11: 121,421,605		probably null	Het
Gm2035	G	A	12: 87,919,722	R46W	possibly damaging	Het
Gmnc	C	T	16: 26,960,529	D249N	probably benign	Het
Gsn	C	T	2: 35,294,948	A305V	probably benign	Het
Haus6	A	T	4: 86,583,752	H627Q	possibly damaging	Het
Heg1	A	G	16: 33,738,550		probably null	Het
Hoxd9	G	T	2: 74,698,365	V104L	possibly damaging	Het
Igkv10-96	A	C	6: 68,632,216	S32A	probably benign	Het
Kcnd2	G	A	6: 21,216,437	V47M	probably damaging	Het
Mab21l3	C	T	3: 101,815,153	V386M	probably damaging	Het
Masp2	A	G	4: 148,612,157	S404G	probably benign	Het
Olfr1024	T	A	2: 85,904,142	Q304L	probably benign	Het
Olfr1454	A	G	19: 13,064,316	I302V	probably benign	Het
Olfr835	G	T	9: 19,035,332	D70Y	probably damaging	Het
P4htm	A	T	9: 108,581,860	M291K	possibly damaging	Het
Pde6c	T	C	19: 38,133,090	S49P	probably benign	Het
Pdzd7	A	G	19: 45,037,114	V314A	probably benign	Het
Pfkl	G	A	10: 78,002,082	R31*	probably null	Het
Phlpp2	C	A	8: 109,939,953	P1038Q	probably damaging	Het
Pik3c2b	T	C	1: 133,066,465	S56P	probably benign	Het
Plec	A	G	15: 76,205,705	V145A	unknown	Het
Prg3	G	A	2: 84,991,504	V158I	probably benign	Het
Prg3	G	T	2: 84,993,023	D181Y	probably damaging	Het
Rbp3	A	G	14: 33,955,204	T370A	probably benign	Het
Rgl2	T	C	17: 33,934,990	F457L	possibly damaging	Het
Rubcnl	T	A	14: 75,049,626	M578K	probably damaging	Het
Siae	G	A	9: 37,616,946	V72M	possibly damaging	Het
Smchd1	A	T	17: 71,353,516	D1864E	probably benign	Het
Smox	T	C	2: 131,520,566	I255T	possibly damaging	Het
Tas2r123	A	G	6: 132,847,698	N186S	possibly damaging	Het
Thbs2	T	A	17: 14,690,116	I74F	possibly damaging	Het
Timm44	T	C	8: 4,267,311	D238G	probably damaging	Het
Tlk2	T	C	11: 105,221,359		probably null	Het
Tnc	A	G	4: 64,013,128	S782P	probably damaging	Het
Tpr	A	T	1: 150,406,551	K336N	probably damaging	Het
Uggt2	A	T	14: 119,019,637		probably null	Het
Vmn2r101	T	A	17: 19,612,178	I812N	probably damaging	Het
Vps33a	T	C	5: 123,535,215	Q436R	probably null	Het
Ywhaq	T	C	12: 21,416,869	K75E	possibly damaging	Het

Other mutations in Zfp87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02165:Zfp87	APN	13	67517870	missense	probably damaging	0.99
IGL02372:Zfp87	APN	13	67520620	splice site	probably benign
IGL03160:Zfp87	APN	13	67521273	missense	probably damaging	1.00
R3801:Zfp87	UTSW	13	67521215	missense	probably damaging	1.00
R4950:Zfp87	UTSW	13	67517899	missense	probably benign	0.20
R5604:Zfp87	UTSW	13	67517826	missense	probably damaging	1.00
R6392:Zfp87	UTSW	13	67516867	missense	probably benign	0.00
R7009:Zfp87	UTSW	13	67517054	missense	probably damaging	1.00
R7448:Zfp87	UTSW	13	67517044	missense	probably benign	0.01
R7597:Zfp87	UTSW	13	67517293	missense	probably benign	0.06
R9780:Zfp87	UTSW	13	67517122	missense	probably damaging	1.00
Z1176:Zfp87	UTSW	13	67526156	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- GGTACGAATGAGTAGAAAAGGCTTT -3'
(R):5'- CGCTTGGAGCTCATTCATATCTCAG -3'

Sequencing Primer
(F):5'- TGAGTAGAAAAGGCTTTCCCGC -3'
(R):5'- TCTGCAGAGAATCCTTACAGGTG -3'

Posted On

2019-06-26