Mutagenetix > Incidental Mutation

Incidental Mutation 'R7804:Vmn2r14'

600661

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

045859-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.526) question?

Stock #

R7804 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109368324 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Methionine at position 223 (L223M)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably benign
Transcript: ENSMUST00000170341
AA Change: L223M

PolyPhen 2 Score 0.411 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: L223M

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

95% (55/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933415A04Rik	GTGTGTATGTGT	GTGTGT	11: 43,478,241 (GRCm39)		probably benign	Het
Ago4	A	T	4: 126,406,423 (GRCm39)	C296S	probably benign	Het
Apbb1	A	G	7: 105,215,807 (GRCm39)	L401P	probably damaging	Het
Cacnb2	C	A	2: 14,972,848 (GRCm39)	P252T	probably benign	Het
Ctnna2	C	T	6: 77,618,357 (GRCm39)	V202I	probably benign	Het
Dapk1	A	T	13: 60,873,153 (GRCm39)	I352F	probably benign	Het
Dexi	T	C	16: 10,360,349 (GRCm39)	D69G	possibly damaging	Het
Dlg5	T	C	14: 24,215,388 (GRCm39)	E645G	possibly damaging	Het
Dnah3	A	G	7: 119,551,841 (GRCm39)	I2826T	probably damaging	Het
Dnah3	A	G	7: 119,610,235 (GRCm39)	W1734R	probably damaging	Het
Dtna	A	G	18: 23,728,666 (GRCm39)	K287R	probably damaging	Het
Elovl5	A	G	9: 77,887,105 (GRCm39)		probably null	Het
Ess2	T	A	16: 17,729,031 (GRCm39)	I47F	probably damaging	Het
Fam222b	C	A	11: 78,044,979 (GRCm39)	P180Q	probably benign	Het
Fat3	C	T	9: 15,901,888 (GRCm39)	V3046I	probably benign	Het
Fem1a	A	G	17: 56,565,068 (GRCm39)	D387G	probably damaging	Het
Frmd5	A	G	2: 121,422,225 (GRCm39)	Y33H	probably damaging	Het
Gucy2g	A	T	19: 55,216,584 (GRCm39)	L464H	probably benign	Het
Ints2	T	C	11: 86,103,489 (GRCm39)	E1189G	possibly damaging	Het
Itgb4	T	C	11: 115,894,510 (GRCm39)	V1355A	probably damaging	Het
Lepr	A	T	4: 101,639,783 (GRCm39)	S750C	probably damaging	Het
Lingo1	T	C	9: 56,526,798 (GRCm39)	D603G	probably benign	Het
Map4k3	A	C	17: 80,922,499 (GRCm39)	V474G	probably damaging	Het
Myl4	T	A	11: 104,475,787 (GRCm39)	V167E	probably damaging	Het
Naa25	T	C	5: 121,562,652 (GRCm39)	V478A	probably benign	Het
Neurl4	T	A	11: 69,796,700 (GRCm39)	L487H	probably benign	Het
Nkx2-9	C	T	12: 56,658,917 (GRCm39)	R99H	probably damaging	Het
Or4c110	C	G	2: 88,831,855 (GRCm39)	R259P	unknown	Het
Or52x1	A	T	7: 104,853,367 (GRCm39)	M61K	probably damaging	Het
Or6c76	A	G	10: 129,612,091 (GRCm39)	I103V	probably benign	Het
Pcdhga9	A	G	18: 37,871,132 (GRCm39)	I320M	probably damaging	Het
Pi15	T	A	1: 17,695,137 (GRCm39)	C251*	probably null	Het
Pkhd1l1	A	T	15: 44,460,534 (GRCm39)	K4248*	probably null	Het
Prkg1	C	T	19: 30,556,032 (GRCm39)	V625I	probably benign	Het
Prkg1	G	A	19: 30,602,170 (GRCm39)	A362V	possibly damaging	Het
Prol1	C	T	5: 88,476,264 (GRCm39)	T218I	unknown	Het
Ptpro	G	A	6: 137,376,599 (GRCm39)		probably null	Het
Rev3l	T	A	10: 39,699,481 (GRCm39)	I1326K	probably benign	Het
Shank1	C	A	7: 43,962,308 (GRCm39)	R60S	unknown	Het
Slc24a2	A	G	4: 86,909,774 (GRCm39)	V648A	probably damaging	Het
Slc35d3	T	C	10: 19,725,116 (GRCm39)	T247A	probably damaging	Het
Specc1	C	T	11: 62,096,223 (GRCm39)	T895I	probably damaging	Het
Spta1	A	G	1: 174,023,471 (GRCm39)	E626G	possibly damaging	Het
Syngr2	C	A	11: 117,703,401 (GRCm39)	S72R	possibly damaging	Het
Synj2	T	A	17: 6,069,809 (GRCm39)	L490Q	unknown	Het
Tbc1d9	T	C	8: 83,963,341 (GRCm39)	L351P	possibly damaging	Het
Tcof1	G	C	18: 60,962,123 (GRCm39)	A702G	possibly damaging	Het
Themis3	G	A	17: 66,862,605 (GRCm39)	T451I	probably benign	Het
Tia1	T	C	6: 86,401,364 (GRCm39)	S146P	probably benign	Het
Tpp2	T	A	1: 44,022,441 (GRCm39)	N946K	probably benign	Het
Tpr	A	T	1: 150,308,310 (GRCm39)	R1688S	probably damaging	Het
Trav12-1	A	G	14: 53,775,988 (GRCm39)	Q47R	possibly damaging	Het
Ttll9	G	A	2: 152,844,278 (GRCm39)		probably null	Het
Ttn	G	A	2: 76,537,585 (GRCm39)	T34781M	possibly damaging	Het
V1ra8	A	G	6: 90,180,298 (GRCm39)	Y167C	probably damaging	Het
Vegfb	T	C	19: 6,963,707 (GRCm39)	D84G	probably damaging	Het
Wdr26	A	G	1: 181,010,387 (GRCm39)	I554T	probably damaging	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109,372,431 (GRCm39)	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109,364,095 (GRCm39)	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109,367,816 (GRCm39)	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7608:Vmn2r14	UTSW	5	109,369,276 (GRCm39)	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- CCATTGTGAGGTTGTGATCCAG -3'
(R):5'- GGACCATTTAATCCTAAACTGAGTGAC -3'

Sequencing Primer
(F):5'- CACCTTCTAAAGCTGACTTCTAGGG -3'
(R):5'- ACTGAGTGACCATGACCAGTTTC -3'

Posted On

2019-11-26