Mutagenetix > Incidental Mutations

Incidental Mutation 'R1061:Cyp3a25'

94462

Institutional Source

Beutler Lab

Gene Symbol

Cyp3a25

Ensembl Gene

ENSMUSG00000029630

Gene Name

cytochrome P450, family 3, subfamily a, polypeptide 25

Synonyms

MMRRC Submission

039147-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.089) question?

Stock #

R1061 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

145977194-146009618 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 145986833 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 333 (D333E)

Ref Sequence

ENSEMBL: ENSMUSP00000065585 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068317] [ENSMUST00000138870] [ENSMUST00000145062]

AlphaFold

O09158

Predicted Effect

probably benign
Transcript: ENSMUST00000068317
AA Change: D333E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000065585
Gene: ENSMUSG00000029630
AA Change: D333E

Domain	Start	End	E-Value	Type
Pfam:p450	38	493	9.4e-129	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000138870

SMART Domains

Protein: ENSMUSP00000116077
Gene: ENSMUSG00000029630

Domain	Start	End	E-Value	Type
Pfam:p450	38	126	2e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145062

SMART Domains

Protein: ENSMUSP00000123615
Gene: ENSMUSG00000029630

Domain	Start	End	E-Value	Type
Pfam:p450	38	148	3.9e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153808

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 93.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,277,809	A715T	probably benign	Het
Ampd2	A	G	3: 108,075,689	Y641H	probably damaging	Het
Aqp4	A	T	18: 15,398,191	V171E	probably damaging	Het
Arid4a	T	A	12: 71,074,955	S381R	probably damaging	Het
Armc8	T	G	9: 99,537,731	N51H	probably damaging	Het
Asic5	T	A	3: 82,021,001	Y424N	probably damaging	Het
Bcl11a	A	T	11: 24,164,069	K471*	probably null	Het
Birc6	A	G	17: 74,689,312	T4494A	probably damaging	Het
Btbd9	A	G	17: 30,527,435	I139T	probably benign	Het
Ccdc114	T	C	7: 45,941,755	I218T	probably damaging	Het
Ccdc60	C	A	5: 116,172,468	R178S	possibly damaging	Het
Cd163	C	T	6: 124,309,169	A226V	probably benign	Het
Cog1	T	G	11: 113,652,037	S170A	probably benign	Het
Cwc15	T	C	9: 14,507,915	L169P	probably damaging	Het
Ddc	C	T	11: 11,829,132	V331I	probably benign	Het
Dlc1	T	A	8: 36,858,051	T367S	probably benign	Het
Dnah17	T	C	11: 118,052,688	D3183G	possibly damaging	Het
Eif2a	T	A	3: 58,545,065	Y165*	probably null	Het
Eml6	T	C	11: 29,777,267	D1285G	probably damaging	Het
Fam92b	C	A	8: 120,169,704		probably null	Het
Fasn	T	C	11: 120,822,182		probably null	Het
Fbn1	T	C	2: 125,345,963	T1549A	probably benign	Het
Fezf2	T	C	14: 12,342,713	Y384C	probably damaging	Het
Flvcr1	A	T	1: 191,008,173	V550E	probably benign	Het
Gcm2	A	G	13: 41,105,871	W41R	probably damaging	Het
Gli2	A	T	1: 118,854,517	M160K	possibly damaging	Het
Gpr61	C	T	3: 108,150,307	R346H	probably damaging	Het
Hoxa5	A	T	6: 52,204,155	S66T	probably benign	Het
Hsph1	A	T	5: 149,618,418	V781D	possibly damaging	Het
Kdm3b	G	A	18: 34,796,862	V220M	probably damaging	Het
Klhl25	T	A	7: 75,866,520	Y391*	probably null	Het
Mc2r	A	T	18: 68,407,809	Y138N	probably damaging	Het
Mtap	C	A	4: 89,156,584	D106E	probably benign	Het
Nup43	G	T	10: 7,667,671	W37L	probably damaging	Het
Nxpe2	T	A	9: 48,326,363	E197D	probably damaging	Het
Olfr1098	A	T	2: 86,922,782	V250D	possibly damaging	Het
Olfr484	T	C	7: 108,124,456	Y269C	probably damaging	Het
Olfr544	T	A	7: 102,484,114	*335C	probably null	Het
Olfr934	C	A	9: 38,982,483	C187F	probably damaging	Het
Pitrm1	A	G	13: 6,555,575	H186R	probably damaging	Het
Pklr	C	T	3: 89,144,881	R467C	probably damaging	Het
Plxna2	A	G	1: 194,644,093	N112D	probably damaging	Het
Ppp1r16a	C	T	15: 76,693,669	Q328*	probably null	Het
Ppp3cb	A	C	14: 20,508,614		probably null	Het
R3hcc1l	C	T	19: 42,583,426	R135*	probably null	Het
Ralyl	A	C	3: 14,115,701	D68A	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,110,324		probably benign	Het
S100a1	T	C	3: 90,511,312	N65S	probably damaging	Het
Scn11a	A	T	9: 119,795,663	M531K	probably damaging	Het
Slc5a5	T	A	8: 70,890,221	M232L	probably benign	Het
Tet3	A	T	6: 83,373,323	N1054K	probably damaging	Het
Tg	A	G	15: 66,698,559	N1427D	probably benign	Het
Tns1	A	C	1: 73,917,672	V530G	probably damaging	Het
Trcg1	C	A	9: 57,245,873	Q600K	possibly damaging	Het
Tshz3	A	G	7: 36,768,706	E40G	probably damaging	Het
Usp34	T	C	11: 23,384,420	F1138S	possibly damaging	Het
Vmn2r26	C	T	6: 124,061,644	T726I	probably benign	Het
Vmn2r97	A	T	17: 18,928,178	R112*	probably null	Het
Vwa2	G	T	19: 56,908,994	R577L	probably benign	Het
Vwa3b	A	C	1: 37,157,430	Q46P	probably damaging	Het
Wdr25	T	A	12: 108,992,799		probably null	Het
Wrap53	A	G	11: 69,562,400	L405P	probably damaging	Het
Zfp629	C	A	7: 127,611,989	W216L	probably damaging	Het

Other mutations in Cyp3a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Cyp3a25	APN	5	146001463	nonsense	probably null
IGL00430:Cyp3a25	APN	5	145993360	missense	probably damaging	1.00
IGL00803:Cyp3a25	APN	5	146001443	splice site	probably benign
IGL00928:Cyp3a25	APN	5	145986954	missense	possibly damaging	0.94
IGL01557:Cyp3a25	APN	5	145984901	missense	probably damaging	1.00
IGL01997:Cyp3a25	APN	5	145994956	missense	possibly damaging	0.92
IGL02140:Cyp3a25	APN	5	146009463	splice site	probably benign
IGL02267:Cyp3a25	APN	5	145998552	missense	possibly damaging	0.48
IGL02272:Cyp3a25	APN	5	145993265	intron	probably benign
IGL02327:Cyp3a25	APN	5	145986921	missense	possibly damaging	0.50
IGL02411:Cyp3a25	APN	5	146001447	critical splice donor site	probably benign
IGL02504:Cyp3a25	APN	5	145993331	missense	probably benign	0.03
IGL02653:Cyp3a25	APN	5	146003110	missense	possibly damaging	0.95
R0378:Cyp3a25	UTSW	5	145986842	missense	probably damaging	1.00
R0403:Cyp3a25	UTSW	5	145998513	missense	probably damaging	1.00
R0685:Cyp3a25	UTSW	5	145998546	missense	probably damaging	1.00
R0725:Cyp3a25	UTSW	5	145994936	missense	probably damaging	1.00
R0798:Cyp3a25	UTSW	5	145991533	missense	probably damaging	0.98
R1519:Cyp3a25	UTSW	5	146001447	critical splice donor site	probably null
R1628:Cyp3a25	UTSW	5	146001463	nonsense	probably null
R1822:Cyp3a25	UTSW	5	145984953	missense	probably damaging	1.00
R1824:Cyp3a25	UTSW	5	145984953	missense	probably damaging	1.00
R1864:Cyp3a25	UTSW	5	145994929	missense	probably damaging	0.98
R2062:Cyp3a25	UTSW	5	145986969	splice site	probably benign
R2401:Cyp3a25	UTSW	5	145986968	critical splice acceptor site	probably null
R2516:Cyp3a25	UTSW	5	146003027	splice site	probably null
R3080:Cyp3a25	UTSW	5	145998531	missense	probably benign	0.33
R3236:Cyp3a25	UTSW	5	146003128	splice site	probably benign
R3694:Cyp3a25	UTSW	5	145989976	splice site	probably null
R3730:Cyp3a25	UTSW	5	146003081	missense	probably damaging	1.00
R4112:Cyp3a25	UTSW	5	146003031	missense	probably benign	0.18
R4258:Cyp3a25	UTSW	5	145991438	missense	probably damaging	1.00
R4651:Cyp3a25	UTSW	5	145994891	missense	probably benign	0.01
R4788:Cyp3a25	UTSW	5	145985082	nonsense	probably null
R4899:Cyp3a25	UTSW	5	145977671	missense	possibly damaging	0.59
R4926:Cyp3a25	UTSW	5	145991456	missense	probably damaging	1.00
R4952:Cyp3a25	UTSW	5	145991524	missense	probably benign	0.01
R5270:Cyp3a25	UTSW	5	145981502	missense	probably benign	0.36
R5595:Cyp3a25	UTSW	5	145994863	critical splice donor site	probably null
R5659:Cyp3a25	UTSW	5	145991546	missense	possibly damaging	0.69
R5787:Cyp3a25	UTSW	5	145998503	missense	probably benign	0.14
R6307:Cyp3a25	UTSW	5	145994956	missense	possibly damaging	0.92
R6380:Cyp3a25	UTSW	5	145998547	missense	probably damaging	0.99
R7055:Cyp3a25	UTSW	5	145992991	missense	probably benign	0.00
R7140:Cyp3a25	UTSW	5	146003045	missense	probably benign
R7189:Cyp3a25	UTSW	5	146003060	missense	probably benign	0.37
R7201:Cyp3a25	UTSW	5	145991447	missense	probably benign	0.22
R7201:Cyp3a25	UTSW	5	146003058	missense	probably benign	0.00
R7332:Cyp3a25	UTSW	5	145993007	missense	probably damaging	1.00
R7404:Cyp3a25	UTSW	5	145986825	missense	probably damaging	1.00
R7548:Cyp3a25	UTSW	5	145986925	missense	probably damaging	0.98
R7607:Cyp3a25	UTSW	5	145984981	missense	possibly damaging	0.87
R8022:Cyp3a25	UTSW	5	145977668	missense	probably benign	0.33
R8266:Cyp3a25	UTSW	5	145992986	missense	probably damaging	1.00
R8894:Cyp3a25	UTSW	5	145994860	splice site	probably benign
R9249:Cyp3a25	UTSW	5	145991546	missense	possibly damaging	0.69

Predicted Primers

PCR Primer
(F):5'- CTCAGCCCGTTGAGATCAATGAAGG -3'
(R):5'- CAGTCCCAGTTACTGTGAACTGCC -3'

Sequencing Primer
(F):5'- AGAGTCATACAGATTTCCTGGGTTTC -3'
(R):5'- CTGTTCTCTAGACAGCTAGGAGTAAC -3'

Posted On

2014-01-05