Incidental Mutation 'R5134:Fer1l6'
ID |
396075 |
Institutional Source |
Beutler Lab
|
Gene Symbol |
Fer1l6
|
Ensembl Gene |
ENSMUSG00000037106 |
Gene Name |
fer-1 like family member 6 |
Synonyms |
EG631797 |
MMRRC Submission |
042722-MU
|
Accession Numbers |
|
Essential gene? |
Probably non essential
(E-score: 0.068)
|
Stock # |
R5134 (G1)
|
Quality Score |
225 |
Status
|
Validated
|
Chromosome |
15 |
Chromosomal Location |
58381897-58536936 bp(+) (GRCm39) |
Type of Mutation |
missense |
DNA Base Change (assembly) |
A to T
at 58512003 bp (GRCm39)
|
Zygosity |
Heterozygous |
Amino Acid Change |
Aspartic acid to Valine
at position 1490
(D1490V)
|
Ref Sequence |
ENSEMBL: ENSMUSP00000125718
(fasta)
|
Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000161028]
|
AlphaFold |
E0CZ42 |
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000159705
|
Predicted Effect |
probably damaging
Transcript: ENSMUST00000161028
AA Change: D1490V
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
SMART Domains |
Protein: ENSMUSP00000125718 Gene: ENSMUSG00000037106 AA Change: D1490V
Domain | Start | End | E-Value | Type |
low complexity region
|
4 |
20 |
N/A |
INTRINSIC |
C2
|
83 |
179 |
4.09e-12 |
SMART |
FerI
|
165 |
235 |
2.06e-36 |
SMART |
C2
|
243 |
354 |
5.19e-14 |
SMART |
low complexity region
|
412 |
449 |
N/A |
INTRINSIC |
FerB
|
714 |
787 |
2.53e-45 |
SMART |
C2
|
829 |
936 |
8.84e-8 |
SMART |
C2
|
1000 |
1099 |
3.05e0 |
SMART |
low complexity region
|
1189 |
1203 |
N/A |
INTRINSIC |
low complexity region
|
1256 |
1270 |
N/A |
INTRINSIC |
C2
|
1361 |
1460 |
5.78e-12 |
SMART |
low complexity region
|
1518 |
1529 |
N/A |
INTRINSIC |
C2
|
1601 |
1731 |
1.01e-2 |
SMART |
Pfam:Ferlin_C
|
1765 |
1857 |
2.3e-40 |
PFAM |
|
Meta Mutation Damage Score |
0.2277 |
Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 96.7%
- 20x: 93.5%
|
Validation Efficiency |
96% (100/104) |
Allele List at MGI |
|
Other mutations in this stock |
Total: 89 list
Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
Adarb1 |
T |
A |
10: 77,161,679 (GRCm39) |
|
probably benign |
Het |
Adrb3 |
A |
C |
8: 27,717,798 (GRCm39) |
M217R |
probably damaging |
Het |
Ank2 |
A |
T |
3: 126,757,094 (GRCm39) |
N1054K |
possibly damaging |
Het |
Aox4 |
A |
T |
1: 58,275,835 (GRCm39) |
D389V |
probably benign |
Het |
Aph1a |
G |
T |
3: 95,802,843 (GRCm39) |
G148W |
probably damaging |
Het |
Arsj |
A |
G |
3: 126,231,803 (GRCm39) |
D183G |
probably benign |
Het |
Atg2b |
A |
G |
12: 105,641,209 (GRCm39) |
S76P |
probably damaging |
Het |
Atxn7l1 |
C |
A |
12: 33,422,875 (GRCm39) |
N815K |
probably damaging |
Het |
Cd3d |
A |
T |
9: 44,896,296 (GRCm39) |
E28D |
probably damaging |
Het |
Cir1 |
G |
A |
2: 73,114,847 (GRCm39) |
R404* |
probably null |
Het |
Cops8 |
A |
G |
1: 90,538,724 (GRCm39) |
D51G |
probably damaging |
Het |
Csgalnact1 |
T |
C |
8: 68,913,623 (GRCm39) |
E194G |
probably benign |
Het |
Cstf2t |
T |
A |
19: 31,061,494 (GRCm39) |
D343E |
probably damaging |
Het |
Ddx23 |
A |
T |
15: 98,548,651 (GRCm39) |
D352E |
possibly damaging |
Het |
Dhx34 |
G |
T |
7: 15,952,175 (GRCm39) |
A150D |
possibly damaging |
Het |
Dnal4 |
A |
T |
15: 79,647,766 (GRCm39) |
V32E |
possibly damaging |
Het |
Dvl3 |
G |
T |
16: 20,343,357 (GRCm39) |
|
probably benign |
Het |
Ehmt1 |
G |
T |
2: 24,767,509 (GRCm39) |
P135T |
probably damaging |
Het |
Enpp2 |
C |
T |
15: 54,762,726 (GRCm39) |
R174H |
probably damaging |
Het |
Eps15 |
G |
A |
4: 109,223,727 (GRCm39) |
|
probably benign |
Het |
F10 |
T |
C |
8: 13,105,698 (GRCm39) |
V421A |
probably damaging |
Het |
Fabp3 |
C |
T |
4: 130,206,180 (GRCm39) |
T57I |
probably benign |
Het |
Fsd1l |
A |
G |
4: 53,647,766 (GRCm39) |
K70E |
probably benign |
Het |
Galnt12 |
G |
A |
4: 47,113,818 (GRCm39) |
A79T |
probably damaging |
Het |
Galnt17 |
A |
T |
5: 130,992,873 (GRCm39) |
M347K |
probably damaging |
Het |
Glyatl3 |
A |
G |
17: 41,215,921 (GRCm39) |
V195A |
probably benign |
Het |
Gm10770 |
A |
T |
2: 150,021,480 (GRCm39) |
|
probably null |
Het |
Gm5578 |
A |
T |
6: 112,583,046 (GRCm39) |
|
noncoding transcript |
Het |
Grn |
C |
A |
11: 102,321,380 (GRCm39) |
|
probably benign |
Het |
Gsk3b |
G |
A |
16: 38,060,882 (GRCm39) |
R418H |
probably damaging |
Het |
Hspa12b |
A |
G |
2: 130,981,428 (GRCm39) |
E221G |
possibly damaging |
Het |
Ighv1-50 |
T |
G |
12: 115,083,653 (GRCm39) |
Q22H |
probably benign |
Het |
Ipcef1 |
A |
G |
10: 6,869,950 (GRCm39) |
I150T |
probably benign |
Het |
Ipp |
T |
A |
4: 116,372,654 (GRCm39) |
F228I |
possibly damaging |
Het |
Itgb7 |
A |
G |
15: 102,125,842 (GRCm39) |
C596R |
probably damaging |
Het |
Kif27 |
T |
C |
13: 58,438,904 (GRCm39) |
D1219G |
possibly damaging |
Het |
Lats1 |
T |
A |
10: 7,567,575 (GRCm39) |
D115E |
probably benign |
Het |
Lpcat3 |
A |
G |
6: 124,679,493 (GRCm39) |
N331S |
probably benign |
Het |
Lrpprc |
A |
T |
17: 85,058,684 (GRCm39) |
N725K |
probably benign |
Het |
Lss |
T |
G |
10: 76,382,070 (GRCm39) |
|
probably benign |
Het |
Mfsd6 |
T |
C |
1: 52,747,515 (GRCm39) |
Y450C |
possibly damaging |
Het |
Mmp21 |
A |
T |
7: 133,280,742 (GRCm39) |
V76E |
probably benign |
Het |
Mtmr11 |
A |
T |
3: 96,077,223 (GRCm39) |
Y527F |
probably damaging |
Het |
Nat10 |
A |
T |
2: 103,573,638 (GRCm39) |
F327I |
probably benign |
Het |
Nfkbiz |
A |
T |
16: 55,638,863 (GRCm39) |
M199K |
probably damaging |
Het |
Nuak1 |
A |
G |
10: 84,210,214 (GRCm39) |
Y625H |
probably benign |
Het |
Odf1 |
A |
G |
15: 38,226,393 (GRCm39) |
T98A |
possibly damaging |
Het |
Oprl1 |
T |
A |
2: 181,360,403 (GRCm39) |
I153N |
probably damaging |
Het |
Or10g3b |
T |
C |
14: 52,587,248 (GRCm39) |
N85S |
probably benign |
Het |
Or1j4 |
A |
T |
2: 36,740,488 (GRCm39) |
L143F |
probably benign |
Het |
Or4x13 |
T |
A |
2: 90,231,107 (GRCm39) |
M34K |
probably benign |
Het |
Osbpl8 |
T |
A |
10: 111,124,554 (GRCm39) |
D705E |
probably benign |
Het |
Otud4 |
G |
A |
8: 80,382,318 (GRCm39) |
V176I |
probably damaging |
Het |
Paics |
T |
C |
5: 77,104,669 (GRCm39) |
|
probably benign |
Het |
Pax5 |
A |
G |
4: 44,710,407 (GRCm39) |
M1T |
probably null |
Het |
Pck1 |
A |
T |
2: 172,995,282 (GRCm39) |
N34I |
probably benign |
Het |
Pde8b |
T |
C |
13: 95,223,250 (GRCm39) |
I335V |
probably benign |
Het |
Piezo2 |
A |
G |
18: 63,207,691 (GRCm39) |
V1440A |
probably damaging |
Het |
Prr23a4 |
T |
C |
9: 98,785,793 (GRCm39) |
S153P |
probably benign |
Het |
Ptpre |
T |
A |
7: 135,253,821 (GRCm39) |
F83Y |
probably damaging |
Het |
Pwwp3a |
T |
C |
10: 80,068,702 (GRCm39) |
L282P |
probably benign |
Het |
Rassf6 |
T |
A |
5: 90,752,225 (GRCm39) |
|
probably null |
Het |
Rilp |
A |
T |
11: 75,403,534 (GRCm39) |
L325F |
probably damaging |
Het |
Rpf1 |
A |
G |
3: 146,212,293 (GRCm39) |
L349S |
probably damaging |
Het |
Rprd2 |
G |
C |
3: 95,672,632 (GRCm39) |
R924G |
probably benign |
Het |
Rps6kc1 |
T |
A |
1: 190,505,845 (GRCm39) |
D1039V |
probably damaging |
Het |
Rufy3 |
G |
A |
5: 88,793,426 (GRCm39) |
V579I |
probably benign |
Het |
Serpinc1 |
A |
G |
1: 160,825,140 (GRCm39) |
|
probably null |
Het |
Siae |
T |
G |
9: 37,557,816 (GRCm39) |
I541S |
possibly damaging |
Het |
St14 |
T |
G |
9: 31,006,879 (GRCm39) |
D649A |
probably benign |
Het |
Stab2 |
A |
G |
10: 86,707,674 (GRCm39) |
|
probably null |
Het |
Sun3 |
T |
C |
11: 8,988,287 (GRCm39) |
T12A |
probably benign |
Het |
Tdrd6 |
A |
G |
17: 43,937,101 (GRCm39) |
Y1316H |
probably damaging |
Het |
Thbs3 |
CAGAAG |
CAG |
3: 89,130,409 (GRCm39) |
|
probably benign |
Het |
Tmem161b |
C |
A |
13: 84,442,887 (GRCm39) |
T269K |
possibly damaging |
Het |
Trav14-3 |
T |
A |
14: 54,000,701 (GRCm39) |
S17T |
unknown |
Het |
Trmu |
T |
A |
15: 85,780,556 (GRCm39) |
|
probably null |
Het |
Ttn |
T |
A |
2: 76,537,586 (GRCm39) |
T26454S |
possibly damaging |
Het |
Ubr3 |
T |
C |
2: 69,850,790 (GRCm39) |
|
probably benign |
Het |
Unc5b |
A |
T |
10: 60,610,879 (GRCm39) |
V376E |
probably benign |
Het |
Usp3 |
T |
C |
9: 66,449,814 (GRCm39) |
S166G |
possibly damaging |
Het |
Vldlr |
C |
A |
19: 27,216,212 (GRCm39) |
C344* |
probably null |
Het |
Vmn1r17 |
A |
G |
6: 57,337,828 (GRCm39) |
V130A |
probably benign |
Het |
Wdfy3 |
A |
C |
5: 102,091,969 (GRCm39) |
Y457D |
probably damaging |
Het |
Zbtb2 |
T |
C |
10: 4,319,267 (GRCm39) |
Y253C |
possibly damaging |
Het |
Zfp687 |
A |
C |
3: 94,917,697 (GRCm39) |
F692V |
probably damaging |
Het |
Zfp758 |
A |
G |
17: 22,594,386 (GRCm39) |
K291E |
probably damaging |
Het |
Zfp799 |
A |
T |
17: 33,039,415 (GRCm39) |
C284S |
probably damaging |
Het |
Zp1 |
C |
A |
19: 10,897,926 (GRCm39) |
C5F |
probably benign |
Het |
|
Other mutations in Fer1l6 |
Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
R0009:Fer1l6
|
UTSW |
15 |
58,534,636 (GRCm39) |
missense |
probably damaging |
1.00 |
R0141:Fer1l6
|
UTSW |
15 |
58,430,251 (GRCm39) |
missense |
probably damaging |
1.00 |
R0178:Fer1l6
|
UTSW |
15 |
58,509,763 (GRCm39) |
splice site |
probably null |
|
R0304:Fer1l6
|
UTSW |
15 |
58,462,411 (GRCm39) |
missense |
probably benign |
0.08 |
R0379:Fer1l6
|
UTSW |
15 |
58,420,187 (GRCm39) |
missense |
probably benign |
0.05 |
R0457:Fer1l6
|
UTSW |
15 |
58,509,943 (GRCm39) |
critical splice donor site |
probably null |
|
R0546:Fer1l6
|
UTSW |
15 |
58,430,257 (GRCm39) |
splice site |
probably null |
|
R0602:Fer1l6
|
UTSW |
15 |
58,449,794 (GRCm39) |
missense |
probably damaging |
0.98 |
R0619:Fer1l6
|
UTSW |
15 |
58,534,784 (GRCm39) |
splice site |
probably null |
|
R0669:Fer1l6
|
UTSW |
15 |
58,425,573 (GRCm39) |
splice site |
probably null |
|
R0854:Fer1l6
|
UTSW |
15 |
58,431,037 (GRCm39) |
missense |
probably benign |
0.00 |
R0948:Fer1l6
|
UTSW |
15 |
58,435,924 (GRCm39) |
missense |
probably benign |
0.00 |
R1180:Fer1l6
|
UTSW |
15 |
58,474,160 (GRCm39) |
splice site |
probably benign |
|
R1483:Fer1l6
|
UTSW |
15 |
58,509,819 (GRCm39) |
missense |
possibly damaging |
0.84 |
R1627:Fer1l6
|
UTSW |
15 |
58,513,728 (GRCm39) |
missense |
probably benign |
0.41 |
R1635:Fer1l6
|
UTSW |
15 |
58,518,930 (GRCm39) |
missense |
probably damaging |
1.00 |
R1834:Fer1l6
|
UTSW |
15 |
58,429,718 (GRCm39) |
missense |
possibly damaging |
0.58 |
R1921:Fer1l6
|
UTSW |
15 |
58,497,080 (GRCm39) |
missense |
probably damaging |
1.00 |
R2000:Fer1l6
|
UTSW |
15 |
58,474,160 (GRCm39) |
splice site |
probably benign |
|
R2041:Fer1l6
|
UTSW |
15 |
58,430,155 (GRCm39) |
missense |
probably damaging |
1.00 |
R2144:Fer1l6
|
UTSW |
15 |
58,499,383 (GRCm39) |
missense |
probably benign |
|
R2145:Fer1l6
|
UTSW |
15 |
58,499,383 (GRCm39) |
missense |
probably benign |
|
R2981:Fer1l6
|
UTSW |
15 |
58,435,926 (GRCm39) |
missense |
probably damaging |
0.99 |
R4164:Fer1l6
|
UTSW |
15 |
58,431,087 (GRCm39) |
missense |
possibly damaging |
0.83 |
R4192:Fer1l6
|
UTSW |
15 |
58,518,998 (GRCm39) |
missense |
probably damaging |
1.00 |
R4273:Fer1l6
|
UTSW |
15 |
58,499,371 (GRCm39) |
missense |
probably benign |
0.41 |
R4573:Fer1l6
|
UTSW |
15 |
58,498,129 (GRCm39) |
critical splice donor site |
probably null |
|
R4581:Fer1l6
|
UTSW |
15 |
58,512,075 (GRCm39) |
missense |
probably damaging |
1.00 |
R4624:Fer1l6
|
UTSW |
15 |
58,425,554 (GRCm39) |
missense |
probably damaging |
1.00 |
R4755:Fer1l6
|
UTSW |
15 |
58,512,060 (GRCm39) |
missense |
probably benign |
0.09 |
R4774:Fer1l6
|
UTSW |
15 |
58,449,798 (GRCm39) |
missense |
probably damaging |
0.99 |
R4894:Fer1l6
|
UTSW |
15 |
58,490,751 (GRCm39) |
missense |
probably damaging |
1.00 |
R4896:Fer1l6
|
UTSW |
15 |
58,509,869 (GRCm39) |
missense |
probably damaging |
1.00 |
R4921:Fer1l6
|
UTSW |
15 |
58,472,160 (GRCm39) |
critical splice acceptor site |
probably null |
|
R4962:Fer1l6
|
UTSW |
15 |
58,443,250 (GRCm39) |
missense |
probably benign |
0.03 |
R5029:Fer1l6
|
UTSW |
15 |
58,515,769 (GRCm39) |
missense |
probably benign |
0.00 |
R5175:Fer1l6
|
UTSW |
15 |
58,422,126 (GRCm39) |
missense |
probably damaging |
1.00 |
R5227:Fer1l6
|
UTSW |
15 |
58,453,752 (GRCm39) |
nonsense |
probably null |
|
R5561:Fer1l6
|
UTSW |
15 |
58,532,674 (GRCm39) |
missense |
probably damaging |
0.97 |
R5621:Fer1l6
|
UTSW |
15 |
58,430,175 (GRCm39) |
missense |
probably damaging |
1.00 |
R5670:Fer1l6
|
UTSW |
15 |
58,494,331 (GRCm39) |
missense |
probably benign |
0.00 |
R5745:Fer1l6
|
UTSW |
15 |
58,443,238 (GRCm39) |
missense |
probably benign |
0.01 |
R5807:Fer1l6
|
UTSW |
15 |
58,462,399 (GRCm39) |
nonsense |
probably null |
|
R5823:Fer1l6
|
UTSW |
15 |
58,462,352 (GRCm39) |
nonsense |
probably null |
|
R5892:Fer1l6
|
UTSW |
15 |
58,435,917 (GRCm39) |
missense |
probably benign |
|
R6006:Fer1l6
|
UTSW |
15 |
58,518,893 (GRCm39) |
missense |
probably damaging |
1.00 |
R6137:Fer1l6
|
UTSW |
15 |
58,431,055 (GRCm39) |
missense |
probably damaging |
0.97 |
R6195:Fer1l6
|
UTSW |
15 |
58,509,806 (GRCm39) |
missense |
probably damaging |
1.00 |
R6234:Fer1l6
|
UTSW |
15 |
58,432,488 (GRCm39) |
missense |
probably damaging |
1.00 |
R6237:Fer1l6
|
UTSW |
15 |
58,509,855 (GRCm39) |
missense |
probably damaging |
1.00 |
R6237:Fer1l6
|
UTSW |
15 |
58,497,026 (GRCm39) |
nonsense |
probably null |
|
R6271:Fer1l6
|
UTSW |
15 |
58,513,767 (GRCm39) |
missense |
probably benign |
0.01 |
R6336:Fer1l6
|
UTSW |
15 |
58,431,081 (GRCm39) |
nonsense |
probably null |
|
R6784:Fer1l6
|
UTSW |
15 |
58,443,275 (GRCm39) |
missense |
possibly damaging |
0.63 |
R6852:Fer1l6
|
UTSW |
15 |
58,466,727 (GRCm39) |
missense |
probably damaging |
1.00 |
R7030:Fer1l6
|
UTSW |
15 |
58,501,227 (GRCm39) |
missense |
probably damaging |
1.00 |
R7088:Fer1l6
|
UTSW |
15 |
58,435,899 (GRCm39) |
missense |
possibly damaging |
0.69 |
R7181:Fer1l6
|
UTSW |
15 |
58,447,146 (GRCm39) |
missense |
probably benign |
0.00 |
R7226:Fer1l6
|
UTSW |
15 |
58,462,384 (GRCm39) |
missense |
probably benign |
0.00 |
R7266:Fer1l6
|
UTSW |
15 |
58,499,446 (GRCm39) |
missense |
probably benign |
|
R7463:Fer1l6
|
UTSW |
15 |
58,445,450 (GRCm39) |
nonsense |
probably null |
|
R7464:Fer1l6
|
UTSW |
15 |
58,445,096 (GRCm39) |
splice site |
probably null |
|
R7469:Fer1l6
|
UTSW |
15 |
58,462,419 (GRCm39) |
splice site |
probably null |
|
R7483:Fer1l6
|
UTSW |
15 |
58,513,794 (GRCm39) |
missense |
possibly damaging |
0.83 |
R7491:Fer1l6
|
UTSW |
15 |
58,472,281 (GRCm39) |
missense |
probably damaging |
1.00 |
R7534:Fer1l6
|
UTSW |
15 |
58,509,875 (GRCm39) |
missense |
probably damaging |
1.00 |
R7562:Fer1l6
|
UTSW |
15 |
58,432,331 (GRCm39) |
missense |
probably benign |
0.00 |
R7580:Fer1l6
|
UTSW |
15 |
58,430,245 (GRCm39) |
missense |
probably benign |
0.41 |
R7599:Fer1l6
|
UTSW |
15 |
58,499,438 (GRCm39) |
missense |
probably benign |
|
R7607:Fer1l6
|
UTSW |
15 |
58,534,581 (GRCm39) |
nonsense |
probably null |
|
R7677:Fer1l6
|
UTSW |
15 |
58,474,139 (GRCm39) |
missense |
probably benign |
0.00 |
R8202:Fer1l6
|
UTSW |
15 |
58,502,486 (GRCm39) |
missense |
probably damaging |
1.00 |
R8261:Fer1l6
|
UTSW |
15 |
58,432,345 (GRCm39) |
missense |
possibly damaging |
0.84 |
R8847:Fer1l6
|
UTSW |
15 |
58,414,012 (GRCm39) |
missense |
possibly damaging |
0.72 |
R9022:Fer1l6
|
UTSW |
15 |
58,455,329 (GRCm39) |
missense |
probably damaging |
0.99 |
R9030:Fer1l6
|
UTSW |
15 |
58,502,594 (GRCm39) |
missense |
probably damaging |
1.00 |
R9160:Fer1l6
|
UTSW |
15 |
58,515,715 (GRCm39) |
missense |
possibly damaging |
0.94 |
R9180:Fer1l6
|
UTSW |
15 |
58,494,230 (GRCm39) |
missense |
probably benign |
0.19 |
R9289:Fer1l6
|
UTSW |
15 |
58,490,766 (GRCm39) |
missense |
probably damaging |
1.00 |
R9559:Fer1l6
|
UTSW |
15 |
58,429,759 (GRCm39) |
missense |
possibly damaging |
0.88 |
R9562:Fer1l6
|
UTSW |
15 |
58,490,370 (GRCm39) |
missense |
possibly damaging |
0.70 |
R9682:Fer1l6
|
UTSW |
15 |
58,422,113 (GRCm39) |
missense |
probably benign |
0.03 |
R9775:Fer1l6
|
UTSW |
15 |
58,497,098 (GRCm39) |
missense |
probably benign |
|
X0021:Fer1l6
|
UTSW |
15 |
58,441,051 (GRCm39) |
nonsense |
probably null |
|
X0027:Fer1l6
|
UTSW |
15 |
58,501,189 (GRCm39) |
missense |
probably damaging |
1.00 |
X0063:Fer1l6
|
UTSW |
15 |
58,490,423 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Predicted Primers |
PCR Primer
(F):5'- GGGGAAATTGCACAAGCCAC -3'
(R):5'- AGCCTTGTGACTGTGAGTTC -3'
Sequencing Primer
(F):5'- TGCACAAGCCACATTTGATAGG -3'
(R):5'- TGTAATTAGATGACCCACTATCCC -3'
|
Posted On |
2016-06-21 |