Incidental Mutation 'R6360:Cpsf1'
| ID |
513089 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Cpsf1
|
| Ensembl Gene |
ENSMUSG00000034022 |
| Gene Name |
cleavage and polyadenylation specific factor 1 |
| Synonyms |
|
| MMRRC Submission |
044510-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.972)
|
| Stock # |
R6360 (G1)
|
| Quality Score |
217.468 |
|
Status
|
Not validated
|
| Chromosome |
15 |
| Chromosomal Location |
76595803-76607591 bp(-) (GRCm38) |
| Type of Mutation |
frame shift |
| DNA Base Change (assembly) |
CCCCTGCATGAGGCAGGTCCC to CCCC
at 76597455 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000155308
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000071898]
[ENSMUST00000160784]
[ENSMUST00000161612]
[ENSMUST00000161732]
[ENSMUST00000162503]
[ENSMUST00000230157]
[ENSMUST00000231042]
|
| AlphaFold |
Q9EPU4 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000071898
|
| SMART Domains |
Protein: ENSMUSP00000071794
Gene: ENSMUSG00000034022
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:MMS1_N
|
92 |
684 |
7.2e-42 |
PFAM |
|
low complexity region
|
902 |
910 |
N/A |
INTRINSIC |
|
Pfam:CPSF_A
|
1071 |
1407 |
4.9e-94 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000159005
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000159049
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000160094
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000160410
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000160784
|
| SMART Domains |
Protein: ENSMUSP00000124666
Gene: ENSMUSG00000022550
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
50 |
69 |
N/A |
INTRINSIC |
|
Pfam:ABC1
|
188 |
304 |
9.2e-38 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000161311
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000161612
|
| SMART Domains |
Protein: ENSMUSP00000124701
Gene: ENSMUSG00000022550
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
50 |
69 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000161732
|
| SMART Domains |
Protein: ENSMUSP00000125482
Gene: ENSMUSG00000022550
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
50 |
69 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000161783
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000162139
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229269
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000229287
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229982
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229797
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000230557
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000230149
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000230822
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000162503
|
| SMART Domains |
Protein: ENSMUSP00000125055
Gene: ENSMUSG00000022550
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
50 |
69 |
N/A |
INTRINSIC |
|
Pfam:ABC1
|
188 |
304 |
2.3e-38 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229015
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000231009
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000231191
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229437
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229367
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000230157
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231042
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000231037
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000162254
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000230903
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229007
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229504
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000229798
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 98.3%
- 20x: 95.2%
|
| Validation Efficiency |
98% (59/60) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 59 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Arhgap10 |
G |
A |
8: 77,259,202 (GRCm38) |
Q657* |
probably null |
Het |
| Car15 |
T |
C |
16: 17,838,066 (GRCm38) |
T560A |
probably benign |
Het |
| Cass4 |
C |
T |
2: 172,432,611 (GRCm38) |
H769Y |
probably damaging |
Het |
| Cdh6 |
A |
G |
15: 13,041,460 (GRCm38) |
I506T |
possibly damaging |
Het |
| Clstn3 |
G |
T |
6: 124,438,429 (GRCm38) |
R659S |
possibly damaging |
Het |
| Cntnap5b |
C |
T |
1: 100,431,736 (GRCm38) |
R695* |
probably null |
Het |
| Dab2ip |
A |
G |
2: 35,710,266 (GRCm38) |
H355R |
probably benign |
Het |
| Dennd2a |
C |
T |
6: 39,493,142 (GRCm38) |
A539T |
probably benign |
Het |
| Dnajc18 |
T |
C |
18: 35,686,709 (GRCm38) |
E173G |
probably damaging |
Het |
| Dock7 |
T |
C |
4: 98,969,662 (GRCm38) |
I1472V |
probably benign |
Het |
| Esco1 |
A |
T |
18: 10,574,931 (GRCm38) |
F714I |
probably damaging |
Het |
| Fam107a |
T |
G |
14: 8,299,619 (GRCm38) |
H73P |
probably damaging |
Het |
| Fam46b |
T |
C |
4: 133,486,756 (GRCm38) |
F313L |
probably damaging |
Het |
| Flg |
A |
G |
3: 93,290,601 (GRCm38) |
|
probably benign |
Het |
| Fyn |
C |
A |
10: 39,526,883 (GRCm38) |
T217K |
possibly damaging |
Het |
| Gbp9 |
T |
C |
5: 105,083,730 (GRCm38) |
D330G |
probably benign |
Het |
| Gin1 |
T |
C |
1: 97,792,539 (GRCm38) |
S509P |
possibly damaging |
Het |
| Gm15922 |
A |
T |
7: 3,736,504 (GRCm38) |
L455Q |
probably damaging |
Het |
| Gm17067 |
A |
C |
7: 42,708,482 (GRCm38) |
S199A |
probably benign |
Het |
| Grk4 |
A |
T |
5: 34,674,537 (GRCm38) |
K50M |
probably damaging |
Het |
| Inpp4b |
A |
G |
8: 81,902,852 (GRCm38) |
H272R |
probably benign |
Het |
| Ipo7 |
T |
A |
7: 110,027,129 (GRCm38) |
L48Q |
probably damaging |
Het |
| Kbtbd2 |
A |
G |
6: 56,779,206 (GRCm38) |
I515T |
probably damaging |
Het |
| Kcnu1 |
T |
A |
8: 25,861,180 (GRCm38) |
S190R |
possibly damaging |
Het |
| Kpnb1 |
T |
C |
11: 97,173,270 (GRCm38) |
N336S |
probably benign |
Het |
| Lbr |
G |
T |
1: 181,832,155 (GRCm38) |
D158E |
probably benign |
Het |
| Mphosph10 |
T |
C |
7: 64,389,955 (GRCm38) |
Q89R |
probably benign |
Het |
| Nectin3 |
T |
A |
16: 46,411,109 (GRCm38) |
T21S |
probably benign |
Het |
| Numb |
C |
A |
12: 83,797,262 (GRCm38) |
R383L |
probably damaging |
Het |
| Olfr1370 |
A |
T |
13: 21,072,583 (GRCm38) |
N239K |
probably damaging |
Het |
| Olfr461 |
G |
T |
6: 40,544,713 (GRCm38) |
Q89K |
possibly damaging |
Het |
| Park2 |
T |
C |
17: 12,004,052 (GRCm38) |
F363S |
probably damaging |
Het |
| Pcdhb11 |
A |
T |
18: 37,422,159 (GRCm38) |
I181F |
probably benign |
Het |
| Pcgf2 |
T |
A |
11: 97,692,409 (GRCm38) |
|
probably null |
Het |
| Pdzd8 |
T |
C |
19: 59,300,983 (GRCm38) |
T662A |
probably benign |
Het |
| Pex13 |
A |
C |
11: 23,655,690 (GRCm38) |
V180G |
probably benign |
Het |
| Pkp4 |
T |
A |
2: 59,214,747 (GRCm38) |
V22D |
probably benign |
Het |
| Ppp1r32 |
T |
C |
19: 10,479,481 (GRCm38) |
N167D |
probably damaging |
Het |
| Prpf4b |
A |
C |
13: 34,901,433 (GRCm38) |
D954A |
probably damaging |
Het |
| Rfx8 |
T |
C |
1: 39,680,965 (GRCm38) |
I317V |
probably benign |
Het |
| Rnaseh2b |
T |
C |
14: 62,361,419 (GRCm38) |
S198P |
probably damaging |
Het |
| Rock1 |
A |
T |
18: 10,116,778 (GRCm38) |
C453S |
possibly damaging |
Het |
| Scarf1 |
G |
T |
11: 75,515,669 (GRCm38) |
G260W |
probably damaging |
Het |
| Scyl1 |
T |
C |
19: 5,760,571 (GRCm38) |
E538G |
probably damaging |
Het |
| Sec14l5 |
A |
T |
16: 5,172,995 (GRCm38) |
I267F |
probably damaging |
Het |
| Senp6 |
T |
A |
9: 80,113,806 (GRCm38) |
V256D |
probably benign |
Het |
| Sf3b4 |
G |
A |
3: 96,176,728 (GRCm38) |
|
probably benign |
Het |
| Ssh1 |
T |
C |
5: 113,961,347 (GRCm38) |
|
probably null |
Het |
| Tas2r143 |
A |
C |
6: 42,400,835 (GRCm38) |
M200L |
probably benign |
Het |
| Tbc1d22a |
C |
T |
15: 86,214,629 (GRCm38) |
P19S |
probably damaging |
Het |
| Tmc5 |
T |
A |
7: 118,633,966 (GRCm38) |
M1K |
probably null |
Het |
| Tnc |
T |
C |
4: 64,000,733 (GRCm38) |
Y1151C |
probably damaging |
Het |
| Tshz3 |
A |
G |
7: 36,769,441 (GRCm38) |
E285G |
probably damaging |
Het |
| Txndc11 |
C |
T |
16: 11,084,792 (GRCm38) |
V664M |
probably damaging |
Het |
| Ube2g1 |
A |
T |
11: 72,663,082 (GRCm38) |
N20Y |
probably damaging |
Het |
| Ufl1 |
T |
A |
4: 25,265,476 (GRCm38) |
I369L |
probably benign |
Het |
| Vwf |
A |
T |
6: 125,683,526 (GRCm38) |
T2666S |
probably benign |
Het |
| Yif1a |
A |
G |
19: 5,092,341 (GRCm38) |
M259V |
probably benign |
Het |
| Zbtb46 |
T |
C |
2: 181,391,455 (GRCm38) |
D471G |
probably damaging |
Het |
|
| Other mutations in Cpsf1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00499:Cpsf1
|
APN |
15 |
76,600,216 (GRCm38) |
missense |
probably benign |
0.27 |
|
IGL01013:Cpsf1
|
APN |
15 |
76,599,297 (GRCm38) |
nonsense |
probably null |
|
|
IGL01599:Cpsf1
|
APN |
15 |
76,596,541 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02008:Cpsf1
|
APN |
15 |
76,603,091 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02291:Cpsf1
|
APN |
15 |
76,602,821 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02901:Cpsf1
|
APN |
15 |
76,599,496 (GRCm38) |
nonsense |
probably null |
|
|
IGL02929:Cpsf1
|
APN |
15 |
76,602,127 (GRCm38) |
critical splice donor site |
probably null |
|
|
IGL03402:Cpsf1
|
APN |
15 |
76,596,003 (GRCm38) |
splice site |
probably null |
|
|
R0005:Cpsf1
|
UTSW |
15 |
76,600,680 (GRCm38) |
critical splice donor site |
probably null |
|
|
R0044:Cpsf1
|
UTSW |
15 |
76,599,553 (GRCm38) |
missense |
probably benign |
|
|
R0044:Cpsf1
|
UTSW |
15 |
76,599,553 (GRCm38) |
missense |
probably benign |
|
|
R0487:Cpsf1
|
UTSW |
15 |
76,597,002 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0510:Cpsf1
|
UTSW |
15 |
76,603,657 (GRCm38) |
intron |
probably benign |
|
|
R0630:Cpsf1
|
UTSW |
15 |
76,601,971 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0780:Cpsf1
|
UTSW |
15 |
76,600,377 (GRCm38) |
missense |
probably benign |
0.17 |
|
R1617:Cpsf1
|
UTSW |
15 |
76,602,370 (GRCm38) |
nonsense |
probably null |
|
|
R1717:Cpsf1
|
UTSW |
15 |
76,602,566 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R1889:Cpsf1
|
UTSW |
15 |
76,602,156 (GRCm38) |
missense |
probably benign |
0.06 |
|
R1994:Cpsf1
|
UTSW |
15 |
76,603,160 (GRCm38) |
missense |
probably benign |
0.03 |
|
R2168:Cpsf1
|
UTSW |
15 |
76,603,737 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R2359:Cpsf1
|
UTSW |
15 |
76,597,673 (GRCm38) |
missense |
probably benign |
0.02 |
|
R2697:Cpsf1
|
UTSW |
15 |
76,599,329 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2847:Cpsf1
|
UTSW |
15 |
76,602,851 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2848:Cpsf1
|
UTSW |
15 |
76,602,851 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3409:Cpsf1
|
UTSW |
15 |
76,601,781 (GRCm38) |
nonsense |
probably null |
|
|
R3410:Cpsf1
|
UTSW |
15 |
76,601,781 (GRCm38) |
nonsense |
probably null |
|
|
R3815:Cpsf1
|
UTSW |
15 |
76,601,149 (GRCm38) |
missense |
probably benign |
0.22 |
|
R4030:Cpsf1
|
UTSW |
15 |
76,601,779 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R4491:Cpsf1
|
UTSW |
15 |
76,597,722 (GRCm38) |
missense |
possibly damaging |
0.85 |
|
R4615:Cpsf1
|
UTSW |
15 |
76,596,937 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R5227:Cpsf1
|
UTSW |
15 |
76,598,948 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5353:Cpsf1
|
UTSW |
15 |
76,602,571 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5548:Cpsf1
|
UTSW |
15 |
76,597,327 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R5552:Cpsf1
|
UTSW |
15 |
76,599,646 (GRCm38) |
missense |
probably benign |
0.27 |
|
R5746:Cpsf1
|
UTSW |
15 |
76,599,837 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6319:Cpsf1
|
UTSW |
15 |
76,596,967 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6572:Cpsf1
|
UTSW |
15 |
76,597,455 (GRCm38) |
frame shift |
probably null |
|
|
R6574:Cpsf1
|
UTSW |
15 |
76,597,455 (GRCm38) |
frame shift |
probably null |
|
|
R6576:Cpsf1
|
UTSW |
15 |
76,597,455 (GRCm38) |
frame shift |
probably null |
|
|
R6577:Cpsf1
|
UTSW |
15 |
76,597,455 (GRCm38) |
frame shift |
probably null |
|
|
R6588:Cpsf1
|
UTSW |
15 |
76,596,822 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6595:Cpsf1
|
UTSW |
15 |
76,602,510 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6621:Cpsf1
|
UTSW |
15 |
76,603,519 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6880:Cpsf1
|
UTSW |
15 |
76,602,539 (GRCm38) |
missense |
probably benign |
0.06 |
|
R6954:Cpsf1
|
UTSW |
15 |
76,599,496 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7100:Cpsf1
|
UTSW |
15 |
76,596,114 (GRCm38) |
missense |
possibly damaging |
0.73 |
|
R7255:Cpsf1
|
UTSW |
15 |
76,597,543 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7318:Cpsf1
|
UTSW |
15 |
76,597,275 (GRCm38) |
nonsense |
probably null |
|
|
R7371:Cpsf1
|
UTSW |
15 |
76,600,575 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7387:Cpsf1
|
UTSW |
15 |
76,602,566 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7446:Cpsf1
|
UTSW |
15 |
76,601,750 (GRCm38) |
missense |
probably benign |
|
|
R7612:Cpsf1
|
UTSW |
15 |
76,597,009 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7739:Cpsf1
|
UTSW |
15 |
76,600,311 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7878:Cpsf1
|
UTSW |
15 |
76,600,500 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8334:Cpsf1
|
UTSW |
15 |
76,603,587 (GRCm38) |
missense |
probably benign |
0.26 |
|
R8345:Cpsf1
|
UTSW |
15 |
76,601,490 (GRCm38) |
missense |
probably benign |
|
|
R8382:Cpsf1
|
UTSW |
15 |
76,600,951 (GRCm38) |
missense |
probably benign |
|
|
R8403:Cpsf1
|
UTSW |
15 |
76,600,283 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8968:Cpsf1
|
UTSW |
15 |
76,601,969 (GRCm38) |
nonsense |
probably null |
|
|
R8972:Cpsf1
|
UTSW |
15 |
76,597,328 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9257:Cpsf1
|
UTSW |
15 |
76,600,792 (GRCm38) |
missense |
probably benign |
|
|
R9627:Cpsf1
|
UTSW |
15 |
76,599,888 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R9776:Cpsf1
|
UTSW |
15 |
76,602,579 (GRCm38) |
missense |
probably damaging |
1.00 |
|
X0052:Cpsf1
|
UTSW |
15 |
76,596,302 (GRCm38) |
missense |
probably benign |
0.04 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCTCCTTCTCATACAGGACC -3'
(R):5'- TTCCCAATGCCAGGTAAGG -3'
Sequencing Primer
(F):5'- CTCATACAGGACCTTGAATTTGTTC -3'
(R):5'- CCAATGCCAGGTAAGGGTGAATC -3'
|
| Posted On |
2018-04-27 |
Incidental Mutation