Mutagenetix > Incidental Mutation

Incidental Mutation 'R1789:Spag17'

201590

Institutional Source

Beutler Lab

Gene Symbol

Spag17

Ensembl Gene

ENSMUSG00000027867

Gene Name

sperm associated antigen 17

Synonyms

PF6, 4931427F14Rik

MMRRC Submission

039820-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1789 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

99792722-100050638 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 99846672 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 65 (S65R)

Ref Sequence

ENSEMBL: ENSMUSP00000134066 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164539]

AlphaFold

Q5S003

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127518

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164539
AA Change: S65R

PolyPhen 2 Score 0.734 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000134066
Gene: ENSMUSG00000027867
AA Change: S65R

Domain	Start	End	E-Value	Type
low complexity region	155	170	N/A	INTRINSIC
low complexity region	384	400	N/A	INTRINSIC
low complexity region	876	887	N/A	INTRINSIC
coiled coil region	909	964	N/A	INTRINSIC
coiled coil region	1079	1120	N/A	INTRINSIC
low complexity region	1179	1190	N/A	INTRINSIC
low complexity region	1192	1205	N/A	INTRINSIC
low complexity region	1209	1220	N/A	INTRINSIC
low complexity region	1223	1238	N/A	INTRINSIC
low complexity region	1394	1405	N/A	INTRINSIC
low complexity region	1931	1942	N/A	INTRINSIC
Pfam:PapD-like	2171	2277	1.2e-15	PFAM

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.2%
20x: 92.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a central pair protein present in the axonemes of cells with a "9 + 2" organization of microtubules. The encoded protein is required for the proper function of the axoneme. Mutations in the orthologous gene in mice lead to primary ciliary dyskinesia characterized by immotile nasal and tracheal cilia, reduced clearance of nasal mucus, profound respiratory distress, hydrocephalus, and neonatal lethality within twelve hours of birth due to impaired airway mucociliary clearance. Single-nucleotide polymorphisms in this gene are associated with human height and targeted mutations lead to skeletal malformations affecting the limbs in mice, suggesting a role for this gene in skeletal development. [provided by RefSeq, Feb 2017]
PHENOTYPE: Homozygous null mice exhibit immotile respiratory cilia with axoneme structural defects, impaired mucociliary clearance, respiratory distress, pulmonary edema, disrupted alveolar epithelium, enlarged brain ventricles consistent with evolving hydrocephalus, failure to suckle, and neonatal lethality. [provided by MGI curators]

Allele List at MGI

All alleles(1) : Targeted, other(1)

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	G	A	16: 20,184,701 (GRCm39)	P986L	probably damaging	Het
Acad10	A	T	5: 121,769,456 (GRCm39)	Y667N	possibly damaging	Het
Ahi1	G	A	10: 20,839,014 (GRCm39)	G121D	probably benign	Het
Ampd1	T	A	3: 103,006,442 (GRCm39)	I690N	possibly damaging	Het
Amy1	C	A	3: 113,351,814 (GRCm39)	W425L	possibly damaging	Het
Arhgap27	A	T	11: 103,223,831 (GRCm39)	V823E	probably damaging	Het
Arhgef17	A	G	7: 100,579,077 (GRCm39)	S624P	probably damaging	Het
Arid5b	T	C	10: 68,021,897 (GRCm39)	H231R	probably damaging	Het
Aspscr1	C	A	11: 120,579,386 (GRCm39)	T78N	probably damaging	Het
Auts2	T	A	5: 131,501,288 (GRCm39)	T42S	probably damaging	Het
Ccdc171	A	G	4: 83,473,045 (GRCm39)	D158G	probably damaging	Het
Ces4a	G	A	8: 105,864,729 (GRCm39)	G69S	probably damaging	Het
Cfap61	T	C	2: 145,781,913 (GRCm39)		probably null	Het
Chrna5	T	C	9: 54,911,935 (GRCm39)	V245A	possibly damaging	Het
Cntnap5c	G	A	17: 58,320,916 (GRCm39)	G163R	probably damaging	Het
Col5a2	A	G	1: 45,417,465 (GRCm39)		probably null	Het
Col5a2	T	C	1: 45,433,936 (GRCm39)	Q759R	probably damaging	Het
Comp	A	G	8: 70,829,796 (GRCm39)	D340G	probably benign	Het
Cyfip1	T	A	7: 55,576,143 (GRCm39)	D1104E	probably damaging	Het
Dnah11	A	T	12: 118,002,515 (GRCm39)	S308T	probably damaging	Het
Dnah5	A	G	15: 28,270,572 (GRCm39)	H958R	probably benign	Het
Elp3	A	G	14: 65,785,368 (GRCm39)	Y478H	probably damaging	Het
Fam237b	A	T	5: 5,625,652 (GRCm39)	Q116L	possibly damaging	Het
Fat3	T	C	9: 16,288,281 (GRCm39)	Y414C	probably benign	Het
Fbxo10	C	A	4: 45,046,389 (GRCm39)	A574S	probably damaging	Het
Fsip2	G	T	2: 82,807,906 (GRCm39)	L1408F	probably benign	Het
Fubp3	T	C	2: 31,501,747 (GRCm39)	V425A	possibly damaging	Het
Gm7168	A	T	17: 14,169,846 (GRCm39)	R404S	probably benign	Het
Gpd1	T	G	15: 99,621,083 (GRCm39)	F299C	probably damaging	Het
Gpr137	C	T	19: 6,919,425 (GRCm39)		probably benign	Het
Grin3b	T	C	10: 79,809,242 (GRCm39)	S331P	probably benign	Het
Grk3	T	A	5: 113,089,584 (GRCm39)	I281F	probably damaging	Het
Hoxb7	T	A	11: 96,177,607 (GRCm39)	S18R	probably damaging	Het
Igf1r	C	T	7: 67,864,681 (GRCm39)	R1160*	probably null	Het
Itfg1	A	G	8: 86,452,141 (GRCm39)		probably null	Het
Itgb8	T	C	12: 119,166,190 (GRCm39)	I114V	probably benign	Het
Kcnk1	A	G	8: 126,752,123 (GRCm39)	E243G	possibly damaging	Het
Kif27	A	G	13: 58,491,822 (GRCm39)	L439P	probably damaging	Het
Kif2c	T	A	4: 117,024,558 (GRCm39)	Q279L	probably benign	Het
Kmt2d	A	T	15: 98,749,955 (GRCm39)		probably benign	Het
L3mbtl1	A	G	2: 162,816,422 (GRCm39)	T821A	probably benign	Het
Lrrc24	A	T	15: 76,606,778 (GRCm39)	M206K	probably benign	Het
Mamdc4	T	G	2: 25,457,634 (GRCm39)	K460Q	possibly damaging	Het
Maml2	C	G	9: 13,608,641 (GRCm39)	L30V	probably damaging	Het
Mc5r	C	G	18: 68,471,741 (GRCm39)		probably null	Het
Myo10	T	G	15: 25,726,611 (GRCm39)		probably null	Het
Myo1e	T	A	9: 70,246,066 (GRCm39)	L419Q	probably damaging	Het
Myo6	C	A	9: 80,207,854 (GRCm39)	H1115Q	probably damaging	Het
Myo7a	A	G	7: 97,756,302 (GRCm39)	V10A	probably damaging	Het
Myoz2	C	T	3: 122,819,776 (GRCm39)	R61H	probably damaging	Het
Ncdn	G	A	4: 126,645,796 (GRCm39)	R38C	probably damaging	Het
Nckap1	T	A	2: 80,350,900 (GRCm39)	T736S	probably benign	Het
Ncor2	G	A	5: 125,096,954 (GRCm39)	A2325V	probably damaging	Het
Nid2	T	A	14: 19,802,499 (GRCm39)	V140E	possibly damaging	Het
Nlrp9c	C	A	7: 26,079,915 (GRCm39)	D704Y	probably benign	Het
Notch3	T	A	17: 32,377,699 (GRCm39)	S126C	probably damaging	Het
Or2g7	T	C	17: 38,378,839 (GRCm39)	I259T	probably damaging	Het
Or5p60	A	G	7: 107,724,122 (GRCm39)	F116S	probably benign	Het
Or5w10	A	T	2: 87,375,327 (GRCm39)	L187H	probably damaging	Het
Or6c219	A	T	10: 129,781,451 (GRCm39)	L160*	probably null	Het
Or6c6c	T	C	10: 129,541,476 (GRCm39)	M243T	possibly damaging	Het
Or6d15	A	C	6: 116,559,658 (GRCm39)	F83C	probably damaging	Het
Pdzd7	A	G	19: 45,027,667 (GRCm39)	I269T	probably damaging	Het
Phf3	A	T	1: 30,845,287 (GRCm39)	D1299E	probably damaging	Het
Pitx2	T	A	3: 129,012,403 (GRCm39)	Y271N	probably damaging	Het
Polk	T	C	13: 96,633,140 (GRCm39)	E301G	probably damaging	Het
Prkdc	C	A	16: 15,557,388 (GRCm39)	N2230K	probably damaging	Het
Prlr	A	G	15: 10,322,622 (GRCm39)	E170G	probably benign	Het
Prss55	A	G	14: 64,313,179 (GRCm39)	I235T	probably damaging	Het
Psd3	T	C	8: 68,413,217 (GRCm39)	I724V	probably benign	Het
Rabep2	A	G	7: 126,037,971 (GRCm39)	T248A	possibly damaging	Het
Rbm26	T	A	14: 105,354,509 (GRCm39)	K949N	probably benign	Het
Rnf213	T	A	11: 119,331,047 (GRCm39)	D2085E	probably damaging	Het
Serpinb7	A	T	1: 107,378,003 (GRCm39)	H232L	possibly damaging	Het
Slu7	A	G	11: 43,336,069 (GRCm39)	Q484R	probably benign	Het
Smg1	A	G	7: 117,745,021 (GRCm39)	S3044P	possibly damaging	Het
Snrnp48	A	G	13: 38,405,336 (GRCm39)	D248G	possibly damaging	Het
Snrpc	C	A	17: 28,064,193 (GRCm39)	P66Q	unknown	Het
Snrpn	T	A	7: 59,633,207 (GRCm39)		probably benign	Het
Srsf6	C	A	2: 162,776,408 (GRCm39)		probably benign	Het
Stil	T	A	4: 114,898,979 (GRCm39)	M1203K	probably benign	Het
Syt17	T	C	7: 118,036,061 (GRCm39)	T106A	probably benign	Het
Tbx15	C	T	3: 99,259,562 (GRCm39)	Q478*	probably null	Het
Tg	A	T	15: 66,609,397 (GRCm39)	Q319L	probably benign	Het
Thada	A	G	17: 84,755,461 (GRCm39)	L243P	probably damaging	Het
Thada	G	T	17: 84,755,462 (GRCm39)	L243I	probably damaging	Het
Tnnt3	A	G	7: 142,066,101 (GRCm39)	R211G	probably damaging	Het
Togaram1	A	T	12: 65,049,409 (GRCm39)	Q1282L	possibly damaging	Het
Tpm3-rs7	T	G	14: 113,552,379 (GRCm39)	M91R	probably damaging	Het
Trappc9	G	A	15: 72,897,816 (GRCm39)	R377W	probably damaging	Het
Ttll7	T	G	3: 146,621,535 (GRCm39)	L378R	probably damaging	Het
Tyw1	T	G	5: 130,287,834 (GRCm39)	I22R	probably damaging	Het
Ubr3	T	C	2: 69,846,711 (GRCm39)	S1645P	possibly damaging	Het
Ubr4	T	C	4: 139,120,364 (GRCm39)	L263P	probably damaging	Het
Unc13c	T	C	9: 73,663,621 (GRCm39)	E1071G	possibly damaging	Het
Vmn2r44	G	T	7: 8,383,122 (GRCm39)	D157E	possibly damaging	Het
Vps16	C	T	2: 130,285,520 (GRCm39)	T821I	probably benign	Het
Washc4	T	C	10: 83,415,389 (GRCm39)	V793A	possibly damaging	Het
Wdr35	G	T	12: 9,027,435 (GRCm39)		probably null	Het
Zfp277	A	G	12: 40,414,084 (GRCm39)	F254L	probably benign	Het

Other mutations in Spag17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01096:Spag17	APN	3	99,970,691 (GRCm39)	missense	probably benign	0.00
IGL01143:Spag17	APN	3	99,846,614 (GRCm39)	missense	probably benign	0.00
IGL01329:Spag17	APN	3	100,002,865 (GRCm39)	missense	probably benign	0.16
IGL01393:Spag17	APN	3	99,934,926 (GRCm39)	missense	possibly damaging	0.53
IGL01617:Spag17	APN	3	100,016,824 (GRCm39)	missense	possibly damaging	0.65
IGL01705:Spag17	APN	3	99,930,046 (GRCm39)	missense	probably benign	0.01
IGL01928:Spag17	APN	3	99,847,390 (GRCm39)	splice site	probably benign
IGL01981:Spag17	APN	3	99,966,149 (GRCm39)	missense	probably benign	0.03
IGL02435:Spag17	APN	3	99,889,760 (GRCm39)	missense	possibly damaging	0.53
IGL02452:Spag17	APN	3	99,934,707 (GRCm39)	missense	probably benign	0.00
IGL02465:Spag17	APN	3	99,983,187 (GRCm39)	missense	probably damaging	0.96
IGL02615:Spag17	APN	3	99,979,401 (GRCm39)	missense	probably benign	0.09
IGL02751:Spag17	APN	3	99,918,110 (GRCm39)	nonsense	probably null
IGL02803:Spag17	APN	3	100,016,713 (GRCm39)	missense	probably benign
IGL02898:Spag17	APN	3	100,008,702 (GRCm39)	missense	probably benign	0.00
IGL03037:Spag17	APN	3	99,979,486 (GRCm39)	splice site	probably null
IGL03068:Spag17	APN	3	99,987,521 (GRCm39)	missense	probably benign	0.35
IGL03131:Spag17	APN	3	99,918,075 (GRCm39)	missense	possibly damaging	0.85
IGL03224:Spag17	APN	3	99,918,156 (GRCm39)	missense	possibly damaging	0.53
FR4342:Spag17	UTSW	3	99,963,568 (GRCm39)	small insertion	probably benign
FR4342:Spag17	UTSW	3	99,963,565 (GRCm39)	small insertion	probably benign
FR4548:Spag17	UTSW	3	99,963,570 (GRCm39)	small insertion	probably benign
FR4589:Spag17	UTSW	3	99,963,574 (GRCm39)	small insertion	probably benign
FR4589:Spag17	UTSW	3	99,963,561 (GRCm39)	small insertion	probably benign
FR4737:Spag17	UTSW	3	99,963,573 (GRCm39)	small insertion	probably benign
FR4976:Spag17	UTSW	3	99,963,571 (GRCm39)	small insertion	probably benign
FR4976:Spag17	UTSW	3	99,963,570 (GRCm39)	small insertion	probably benign
N/A:Spag17	UTSW	3	99,889,570 (GRCm39)	splice site	probably benign
PIT4504001:Spag17	UTSW	3	100,010,426 (GRCm39)	critical splice acceptor site	probably null
PIT4514001:Spag17	UTSW	3	99,920,527 (GRCm39)	missense	possibly damaging	0.53
R0107:Spag17	UTSW	3	99,958,103 (GRCm39)	missense	possibly damaging	0.72
R0230:Spag17	UTSW	3	100,014,143 (GRCm39)	missense	probably benign	0.08
R0243:Spag17	UTSW	3	99,992,684 (GRCm39)	missense	probably benign	0.04
R0321:Spag17	UTSW	3	100,008,719 (GRCm39)	missense	probably damaging	0.99
R0375:Spag17	UTSW	3	99,934,906 (GRCm39)	missense	probably benign
R0417:Spag17	UTSW	3	99,972,870 (GRCm39)	missense	probably benign	0.11
R0490:Spag17	UTSW	3	99,889,727 (GRCm39)	missense	probably damaging	0.97
R0537:Spag17	UTSW	3	100,032,618 (GRCm39)	missense	probably damaging	0.98
R0714:Spag17	UTSW	3	99,987,472 (GRCm39)	missense	probably damaging	0.97
R0844:Spag17	UTSW	3	99,912,101 (GRCm39)	missense	probably benign
R0919:Spag17	UTSW	3	99,979,259 (GRCm39)	splice site	probably benign
R0926:Spag17	UTSW	3	99,979,432 (GRCm39)	missense	probably benign
R1037:Spag17	UTSW	3	100,010,433 (GRCm39)	missense	probably benign	0.01
R1075:Spag17	UTSW	3	100,000,992 (GRCm39)	missense	probably damaging	0.99
R1109:Spag17	UTSW	3	99,934,667 (GRCm39)	missense	possibly damaging	0.86
R1213:Spag17	UTSW	3	100,002,954 (GRCm39)	missense	probably benign	0.01
R1221:Spag17	UTSW	3	99,889,584 (GRCm39)	missense	possibly damaging	0.72
R1576:Spag17	UTSW	3	99,846,679 (GRCm39)	missense	possibly damaging	0.73
R1586:Spag17	UTSW	3	99,929,068 (GRCm39)	missense	possibly damaging	0.53
R1768:Spag17	UTSW	3	99,934,668 (GRCm39)	missense	possibly damaging	0.53
R1782:Spag17	UTSW	3	99,918,070 (GRCm39)	missense	probably benign	0.02
R1945:Spag17	UTSW	3	99,847,298 (GRCm39)	missense	probably benign
R2065:Spag17	UTSW	3	99,920,524 (GRCm39)	missense	probably benign	0.03
R2118:Spag17	UTSW	3	99,956,556 (GRCm39)	missense	possibly damaging	0.72
R2265:Spag17	UTSW	3	99,969,182 (GRCm39)	splice site	probably null
R2266:Spag17	UTSW	3	99,969,182 (GRCm39)	splice site	probably null
R2267:Spag17	UTSW	3	99,969,182 (GRCm39)	splice site	probably null
R2268:Spag17	UTSW	3	99,969,182 (GRCm39)	splice site	probably null
R2271:Spag17	UTSW	3	100,014,113 (GRCm39)	missense	probably damaging	1.00
R2389:Spag17	UTSW	3	100,014,153 (GRCm39)	missense	probably benign	0.27
R2420:Spag17	UTSW	3	99,934,935 (GRCm39)	missense	probably benign
R2422:Spag17	UTSW	3	99,934,935 (GRCm39)	missense	probably benign
R2423:Spag17	UTSW	3	100,010,772 (GRCm39)	missense	probably benign
R3407:Spag17	UTSW	3	99,992,615 (GRCm39)	missense	probably benign	0.09
R3801:Spag17	UTSW	3	99,961,169 (GRCm39)	missense	possibly damaging	0.53
R3856:Spag17	UTSW	3	100,014,075 (GRCm39)	missense	probably damaging	1.00
R4021:Spag17	UTSW	3	99,956,546 (GRCm39)	missense	probably benign	0.00
R4022:Spag17	UTSW	3	99,956,546 (GRCm39)	missense	probably benign	0.00
R4408:Spag17	UTSW	3	100,010,694 (GRCm39)	missense	probably benign
R4468:Spag17	UTSW	3	99,992,682 (GRCm39)	missense	probably damaging	0.98
R4540:Spag17	UTSW	3	99,995,697 (GRCm39)	missense	probably damaging	1.00
R4621:Spag17	UTSW	3	100,010,559 (GRCm39)	missense	probably benign	0.08
R4622:Spag17	UTSW	3	100,010,559 (GRCm39)	missense	probably benign	0.08
R4756:Spag17	UTSW	3	100,010,701 (GRCm39)	missense	possibly damaging	0.68
R4797:Spag17	UTSW	3	99,891,795 (GRCm39)	missense	possibly damaging	0.70
R4855:Spag17	UTSW	3	99,970,649 (GRCm39)	missense	probably benign	0.02
R4887:Spag17	UTSW	3	99,958,147 (GRCm39)	missense	probably damaging	1.00
R4962:Spag17	UTSW	3	99,934,939 (GRCm39)	missense	probably benign
R5030:Spag17	UTSW	3	99,992,657 (GRCm39)	nonsense	probably null
R5042:Spag17	UTSW	3	99,979,465 (GRCm39)	missense	probably damaging	1.00
R5074:Spag17	UTSW	3	99,987,434 (GRCm39)	missense	possibly damaging	0.94
R5195:Spag17	UTSW	3	100,008,704 (GRCm39)	missense	probably benign	0.16
R5200:Spag17	UTSW	3	99,970,787 (GRCm39)	nonsense	probably null
R5267:Spag17	UTSW	3	99,969,264 (GRCm39)	missense	probably damaging	0.98
R5360:Spag17	UTSW	3	100,016,726 (GRCm39)	missense	probably benign	0.00
R5444:Spag17	UTSW	3	99,963,468 (GRCm39)	missense	probably benign	0.06
R5498:Spag17	UTSW	3	100,010,661 (GRCm39)	missense	possibly damaging	0.83
R5503:Spag17	UTSW	3	99,934,560 (GRCm39)	missense	possibly damaging	0.72
R5540:Spag17	UTSW	3	99,963,588 (GRCm39)	missense	possibly damaging	0.91
R5547:Spag17	UTSW	3	99,963,468 (GRCm39)	missense	probably benign	0.06
R5575:Spag17	UTSW	3	99,961,138 (GRCm39)	missense	possibly damaging	0.85
R5629:Spag17	UTSW	3	99,987,435 (GRCm39)	missense	probably benign	0.33
R5639:Spag17	UTSW	3	99,963,482 (GRCm39)	missense	probably damaging	1.00
R5842:Spag17	UTSW	3	99,846,566 (GRCm39)	missense	possibly damaging	0.85
R5976:Spag17	UTSW	3	100,003,107 (GRCm39)	nonsense	probably null
R6082:Spag17	UTSW	3	100,031,501 (GRCm39)	missense	possibly damaging	0.46
R6228:Spag17	UTSW	3	99,929,918 (GRCm39)	missense	probably benign	0.33
R6254:Spag17	UTSW	3	99,972,901 (GRCm39)	missense	probably benign	0.03
R6321:Spag17	UTSW	3	99,995,743 (GRCm39)	missense	probably benign	0.05
R6446:Spag17	UTSW	3	100,010,448 (GRCm39)	missense	probably benign
R6687:Spag17	UTSW	3	100,000,266 (GRCm39)	missense	probably benign	0.07
R6853:Spag17	UTSW	3	99,920,551 (GRCm39)	missense	possibly damaging	0.86
R6946:Spag17	UTSW	3	99,911,999 (GRCm39)	missense	possibly damaging	0.53
R6953:Spag17	UTSW	3	99,942,291 (GRCm39)	missense	possibly damaging	0.53
R7038:Spag17	UTSW	3	99,891,925 (GRCm39)	missense	probably benign	0.00
R7084:Spag17	UTSW	3	99,846,586 (GRCm39)	missense	probably benign	0.18
R7126:Spag17	UTSW	3	100,008,751 (GRCm39)	missense	probably benign	0.00
R7144:Spag17	UTSW	3	99,934,717 (GRCm39)	splice site	probably null
R7198:Spag17	UTSW	3	100,002,888 (GRCm39)	missense	probably benign	0.02
R7318:Spag17	UTSW	3	99,847,299 (GRCm39)	missense	probably benign	0.00
R7403:Spag17	UTSW	3	99,846,691 (GRCm39)	missense	possibly damaging	0.53
R7409:Spag17	UTSW	3	99,934,547 (GRCm39)	missense	possibly damaging	0.73
R7409:Spag17	UTSW	3	99,941,475 (GRCm39)	missense	probably benign	0.00
R7537:Spag17	UTSW	3	99,846,563 (GRCm39)	missense	possibly damaging	0.96
R7609:Spag17	UTSW	3	100,002,911 (GRCm39)	nonsense	probably null
R7772:Spag17	UTSW	3	99,987,434 (GRCm39)	missense	probably damaging	0.98
R7842:Spag17	UTSW	3	99,961,174 (GRCm39)	missense	probably benign	0.18
R7963:Spag17	UTSW	3	99,929,954 (GRCm39)	missense	probably benign	0.02
R8168:Spag17	UTSW	3	99,942,300 (GRCm39)	missense	possibly damaging	0.96
R8291:Spag17	UTSW	3	99,968,166 (GRCm39)	missense	probably benign
R8347:Spag17	UTSW	3	99,934,957 (GRCm39)	missense	probably benign
R8383:Spag17	UTSW	3	99,992,708 (GRCm39)	missense	probably damaging	0.98
R8474:Spag17	UTSW	3	99,934,586 (GRCm39)	missense	probably benign	0.00
R8528:Spag17	UTSW	3	100,031,501 (GRCm39)	missense	possibly damaging	0.46
R8804:Spag17	UTSW	3	99,874,506 (GRCm39)	missense	probably benign
R8809:Spag17	UTSW	3	99,889,738 (GRCm39)	missense	probably benign	0.33
R8818:Spag17	UTSW	3	99,920,543 (GRCm39)	missense	probably benign	0.02
R8830:Spag17	UTSW	3	100,032,751 (GRCm39)	missense	possibly damaging	0.77
R8890:Spag17	UTSW	3	99,911,994 (GRCm39)	missense	possibly damaging	0.73
R9008:Spag17	UTSW	3	99,934,942 (GRCm39)	missense	possibly damaging	0.73
R9095:Spag17	UTSW	3	99,912,092 (GRCm39)	missense	possibly damaging	0.86
R9143:Spag17	UTSW	3	99,934,906 (GRCm39)	missense	probably benign
R9182:Spag17	UTSW	3	99,966,158 (GRCm39)	missense	possibly damaging	0.92
R9211:Spag17	UTSW	3	100,032,614 (GRCm39)	critical splice acceptor site	probably benign
R9344:Spag17	UTSW	3	100,010,793 (GRCm39)	missense	probably benign	0.01
R9354:Spag17	UTSW	3	99,934,905 (GRCm39)	missense	probably benign
R9527:Spag17	UTSW	3	99,970,777 (GRCm39)	missense	probably damaging	1.00
R9658:Spag17	UTSW	3	99,934,932 (GRCm39)	missense	possibly damaging	0.93
R9738:Spag17	UTSW	3	99,934,526 (GRCm39)	missense	possibly damaging	0.53
X0025:Spag17	UTSW	3	100,008,767 (GRCm39)	missense	probably benign	0.31
Z1088:Spag17	UTSW	3	100,002,946 (GRCm39)	missense	probably benign	0.09
Z1176:Spag17	UTSW	3	99,920,309 (GRCm39)	missense	probably benign	0.18
Z1177:Spag17	UTSW	3	99,995,715 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACACAAAGCTACCCAGTG -3'
(R):5'- ACTGCATTGATGAGAAGGTTAGC -3'

Sequencing Primer
(F):5'- GTGAAGACCACTTAGCCGC -3'
(R):5'- GGGTTTGCGCACTTAAAGTAACCC -3'

Posted On

2014-06-23