Mutagenetix > Incidental Mutation

Incidental Mutation 'R6563:Vmn2r24'

522533

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r24

Ensembl Gene

ENSMUSG00000072780

Gene Name

vomeronasal 2, receptor 24

Synonyms

EG243628

MMRRC Submission

044687-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R6563 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123755930-123793239 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 123781137 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Tyrosine at position 448 (N448Y)

Ref Sequence

ENSEMBL: ENSMUSP00000074602 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075095]

AlphaFold

D3YUI0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000075095
AA Change: N448Y

PolyPhen 2 Score 0.858 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000074602
Gene: ENSMUSG00000072780
AA Change: N448Y

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	469	1.6e-32	PFAM
Pfam:NCD3G	518	571	1.1e-22	PFAM
Pfam:7tm_3	602	839	1.1e-54	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000157847

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap11a	A	T	2: 113,664,247 (GRCm39)	C679S	probably benign	Het
Atg4d	C	T	9: 21,179,756 (GRCm39)	L235F	possibly damaging	Het
Cyp2d22	A	T	15: 82,256,113 (GRCm39)	W174R	probably damaging	Het
Cyp4a14	T	A	4: 115,349,283 (GRCm39)	H259L	probably benign	Het
Dennd3	A	T	15: 73,416,229 (GRCm39)	H493L	probably damaging	Het
Dnm1	T	A	2: 32,202,738 (GRCm39)	D759V	probably damaging	Het
Dvl1	T	A	4: 155,940,710 (GRCm39)	N443K	probably damaging	Het
Dync2h1	A	G	9: 7,120,819 (GRCm39)	V2156A	probably benign	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Ermp1	T	C	19: 29,601,178 (GRCm39)	D523G	probably damaging	Het
Fam83c	A	G	2: 155,672,872 (GRCm39)	V295A	probably damaging	Het
Gphn	T	C	12: 78,727,170 (GRCm39)		probably null	Het
Irs1	T	A	1: 82,266,128 (GRCm39)	N696I	probably damaging	Het
Kmt5c	A	G	7: 4,745,628 (GRCm39)	Y96C	probably damaging	Het
Krt77	G	T	15: 101,771,358 (GRCm39)	T315N	probably damaging	Het
L3mbtl3	A	C	10: 26,178,761 (GRCm39)		probably null	Het
Lama3	A	T	18: 12,670,823 (GRCm39)	Y2409F	probably damaging	Het
Lrrc9	A	G	12: 72,533,169 (GRCm39)		probably null	Het
Ltbp4	A	T	7: 27,008,488 (GRCm39)	N1273K	probably damaging	Het
Mfsd13b	G	A	7: 120,594,690 (GRCm39)	A321T	probably damaging	Het
Mvb12b	A	T	2: 33,715,128 (GRCm39)	H167Q	probably benign	Het
Myo1g	T	A	11: 6,467,146 (GRCm39)	N230Y	possibly damaging	Het
Or7a41	A	T	10: 78,871,051 (GRCm39)	R140S	possibly damaging	Het
Prkar2b	G	T	12: 32,043,785 (GRCm39)		probably null	Het
Pwwp2a	G	T	11: 43,596,592 (GRCm39)	A586S	possibly damaging	Het
Ryr1	A	G	7: 28,794,917 (GRCm39)	V1150A	possibly damaging	Het
Sh3bgr	A	G	16: 96,007,143 (GRCm39)		probably null	Het
Slk	T	C	19: 47,624,908 (GRCm39)		probably null	Het
Snx3	A	G	10: 42,402,032 (GRCm39)	E82G	possibly damaging	Het
Spring1	T	C	5: 118,397,047 (GRCm39)	V92A	possibly damaging	Het
Srcin1	A	T	11: 97,425,600 (GRCm39)	Y486N	possibly damaging	Het
Tecpr2	A	G	12: 110,895,521 (GRCm39)	E336G	probably benign	Het
Terf2ip	G	T	8: 112,744,834 (GRCm39)	V384F	probably damaging	Het
Tnn	T	A	1: 159,915,968 (GRCm39)	S1250C	probably damaging	Het
Tspoap1	A	G	11: 87,667,985 (GRCm39)	E1263G	possibly damaging	Het
Tubgcp5	A	G	7: 55,475,409 (GRCm39)	R932G	possibly damaging	Het
Utp25	C	A	1: 192,800,698 (GRCm39)	R374L	probably damaging	Het
Vmn1r175	A	G	7: 23,508,030 (GRCm39)	I199T	possibly damaging	Het
Vmn1r225	C	T	17: 20,722,763 (GRCm39)	A68V	probably benign	Het

Other mutations in Vmn2r24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r24	APN	6	123,792,596 (GRCm39)	missense	probably damaging	1.00
IGL01382:Vmn2r24	APN	6	123,763,938 (GRCm39)	missense	possibly damaging	0.62
IGL01592:Vmn2r24	APN	6	123,764,445 (GRCm39)	missense	probably benign	0.30
IGL01754:Vmn2r24	APN	6	123,781,120 (GRCm39)	missense	probably damaging	1.00
IGL01939:Vmn2r24	APN	6	123,764,404 (GRCm39)	missense	probably benign
IGL02140:Vmn2r24	APN	6	123,757,631 (GRCm39)	missense	probably damaging	0.98
IGL02272:Vmn2r24	APN	6	123,763,843 (GRCm39)	missense	possibly damaging	0.94
IGL02568:Vmn2r24	APN	6	123,792,812 (GRCm39)	missense	probably benign	0.36
IGL02748:Vmn2r24	APN	6	123,793,057 (GRCm39)	missense	possibly damaging	0.90
IGL03022:Vmn2r24	APN	6	123,755,967 (GRCm39)	missense	probably damaging	0.99
IGL03343:Vmn2r24	APN	6	123,793,070 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r24	UTSW	6	123,792,369 (GRCm39)	frame shift	probably null
R0453:Vmn2r24	UTSW	6	123,757,350 (GRCm39)	critical splice acceptor site	probably null
R0538:Vmn2r24	UTSW	6	123,793,012 (GRCm39)	missense	probably benign	0.32
R0607:Vmn2r24	UTSW	6	123,763,893 (GRCm39)	missense	probably benign
R1381:Vmn2r24	UTSW	6	123,763,692 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn2r24	UTSW	6	123,783,479 (GRCm39)	splice site	probably benign
R1848:Vmn2r24	UTSW	6	123,793,183 (GRCm39)	missense	probably damaging	1.00
R2035:Vmn2r24	UTSW	6	123,793,019 (GRCm39)	missense	probably damaging	1.00
R2077:Vmn2r24	UTSW	6	123,792,358 (GRCm39)	missense	probably damaging	1.00
R2122:Vmn2r24	UTSW	6	123,792,353 (GRCm39)	missense	possibly damaging	0.81
R2145:Vmn2r24	UTSW	6	123,755,972 (GRCm39)	missense	probably benign
R2483:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R2512:Vmn2r24	UTSW	6	123,763,985 (GRCm39)	missense	probably benign	0.01
R3001:Vmn2r24	UTSW	6	123,781,231 (GRCm39)	missense	probably benign	0.00
R3002:Vmn2r24	UTSW	6	123,781,231 (GRCm39)	missense	probably benign	0.00
R3236:Vmn2r24	UTSW	6	123,755,984 (GRCm39)	nonsense	probably null
R3623:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R3624:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R3835:Vmn2r24	UTSW	6	123,764,412 (GRCm39)	missense	probably benign	0.33
R4074:Vmn2r24	UTSW	6	123,764,374 (GRCm39)	missense	possibly damaging	0.92
R4075:Vmn2r24	UTSW	6	123,764,374 (GRCm39)	missense	possibly damaging	0.92
R4812:Vmn2r24	UTSW	6	123,756,144 (GRCm39)	missense	probably benign	0.00
R4825:Vmn2r24	UTSW	6	123,792,739 (GRCm39)	missense	probably benign	0.02
R5351:Vmn2r24	UTSW	6	123,793,223 (GRCm39)	missense	possibly damaging	0.80
R5665:Vmn2r24	UTSW	6	123,763,938 (GRCm39)	missense	possibly damaging	0.62
R5790:Vmn2r24	UTSW	6	123,792,499 (GRCm39)	missense	probably benign
R5808:Vmn2r24	UTSW	6	123,792,597 (GRCm39)	nonsense	probably null
R5879:Vmn2r24	UTSW	6	123,764,226 (GRCm39)	missense	possibly damaging	0.89
R5923:Vmn2r24	UTSW	6	123,792,751 (GRCm39)	missense	probably damaging	0.96
R5969:Vmn2r24	UTSW	6	123,755,981 (GRCm39)	missense	probably benign	0.00
R6050:Vmn2r24	UTSW	6	123,792,691 (GRCm39)	missense	probably damaging	1.00
R6171:Vmn2r24	UTSW	6	123,764,205 (GRCm39)	missense	probably damaging	0.98
R6174:Vmn2r24	UTSW	6	123,793,236 (GRCm39)	missense	probably benign	0.00
R6356:Vmn2r24	UTSW	6	123,783,368 (GRCm39)	missense	possibly damaging	0.93
R6562:Vmn2r24	UTSW	6	123,757,386 (GRCm39)	missense	probably benign	0.01
R6584:Vmn2r24	UTSW	6	123,792,764 (GRCm39)	missense	possibly damaging	0.53
R6630:Vmn2r24	UTSW	6	123,763,981 (GRCm39)	missense	probably benign	0.00
R6803:Vmn2r24	UTSW	6	123,755,960 (GRCm39)	missense	possibly damaging	0.64
R6864:Vmn2r24	UTSW	6	123,756,117 (GRCm39)	missense	possibly damaging	0.89
R7252:Vmn2r24	UTSW	6	123,764,191 (GRCm39)	missense	possibly damaging	0.90
R7369:Vmn2r24	UTSW	6	123,792,638 (GRCm39)	missense	probably damaging	0.99
R7646:Vmn2r24	UTSW	6	123,793,169 (GRCm39)	missense	probably benign	0.20
R7799:Vmn2r24	UTSW	6	123,757,422 (GRCm39)	missense	probably benign	0.00
R7803:Vmn2r24	UTSW	6	123,757,438 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r24	UTSW	6	123,755,949 (GRCm39)	missense	possibly damaging	0.86
R8215:Vmn2r24	UTSW	6	123,756,077 (GRCm39)	missense	probably benign	0.10
R8796:Vmn2r24	UTSW	6	123,757,500 (GRCm39)	missense	probably benign
R9172:Vmn2r24	UTSW	6	123,783,432 (GRCm39)	missense	probably damaging	1.00
R9300:Vmn2r24	UTSW	6	123,793,030 (GRCm39)	missense	possibly damaging	0.46
R9369:Vmn2r24	UTSW	6	123,792,357 (GRCm39)	missense	probably damaging	1.00
R9375:Vmn2r24	UTSW	6	123,792,542 (GRCm39)	missense	probably damaging	1.00
R9523:Vmn2r24	UTSW	6	123,763,950 (GRCm39)	missense	possibly damaging	0.89
R9546:Vmn2r24	UTSW	6	123,764,266 (GRCm39)	missense	probably damaging	0.98
RF006:Vmn2r24	UTSW	6	123,783,378 (GRCm39)	missense	probably damaging	1.00
RF016:Vmn2r24	UTSW	6	123,781,174 (GRCm39)	missense	probably benign	0.04
X0023:Vmn2r24	UTSW	6	123,764,359 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r24	UTSW	6	123,781,155 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn2r24	UTSW	6	123,763,719 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCTAACCTCTACATATTGACCTACTG -3'
(R):5'- ACCTGATTGTGGTGTTCACC -3'

Sequencing Primer
(F):5'- GACCTACTGCTAGCATGTATTAGC -3'
(R):5'- GGTGTTCACCCCAAGTTATGAGC -3'

Posted On

2018-06-06