Mutagenetix > Incidental Mutation

Incidental Mutation 'R7485:Vmn2r104'

580118

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

045559-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R7485 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 20029475 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Asparagine at position 845 (H845N)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably benign
Transcript: ENSMUST00000168050
AA Change: H845N

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: H845N

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

99% (99/100)

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430419D17Rik	C	A	7: 131,228,833	P332Q	probably damaging	Het
Adat3	C	T	10: 80,606,400	A24V	probably benign	Het
Agbl1	A	T	7: 76,589,493	E832D	unknown	Het
Akap8l	G	A	17: 32,335,571	S347L	probably benign	Het
Ankmy1	G	T	1: 92,876,657	A868E	probably damaging	Het
Ankrd49	A	T	9: 14,782,541	L110*	probably null	Het
Aoc3	A	G	11: 101,337,403	T679A	probably damaging	Het
Ap1s3	A	G	1: 79,614,301	Y111H	probably damaging	Het
Arnt	T	A	3: 95,495,348	N777K	probably damaging	Het
Atp4b	A	T	8: 13,386,732	M260K	probably benign	Het
Bcl2l15	A	T	3: 103,833,413	D65V	probably damaging	Het
Bicdl1	G	T	5: 115,663,786	S340*	probably null	Het
Ccdc15	T	C	9: 37,315,278	R467G	probably benign	Het
Ccdc68	T	A	18: 69,969,013	M327K	possibly damaging	Het
Ccdc83	T	C	7: 90,223,930	T406A	probably benign	Het
Ccnf	A	T	17: 24,249,258	V55D	probably damaging	Het
Cldn12	A	G	5: 5,508,008	F140L	probably benign	Het
Copb1	A	C	7: 114,245,485	I213S	possibly damaging	Het
Cps1	G	A	1: 67,139,857	G76D	probably damaging	Het
Ctrc	A	G	4: 141,840,316	W159R	probably damaging	Het
Cul4a	C	T	8: 13,140,279	T572M	possibly damaging	Het
Cyp2c40	A	T	19: 39,807,606	Y109*	probably null	Het
Dcc	A	G	18: 71,420,246	Y780H	probably benign	Het
Dctn1	C	T	6: 83,189,905	A283V	possibly damaging	Het
Dio1	T	C	4: 107,297,677	D134G	probably benign	Het
Dlg5	A	T	14: 24,148,322	D1514E	probably benign	Het
Dlg5	A	T	14: 24,177,839	L338Q	probably damaging	Het
Dlgap2	A	T	8: 14,829,952	K767N	probably damaging	Het
Dst	A	T	1: 34,274,189	I4346F	probably benign	Het
Dzip1l	T	C	9: 99,661,012	F507L	probably benign	Het
Erich2	A	T	2: 70,531,765	D300V	probably damaging	Het
Fam114a2	C	T	11: 57,513,689	G83D	probably damaging	Het
Fam131a	C	T	16: 20,701,694	A299V	probably benign	Het
Fbln2	A	G	6: 91,270,161		probably null	Het
Fbn2	T	C	18: 58,071,840	D1177G	possibly damaging	Het
Fbxl6	T	C	15: 76,537,913		probably null	Het
Frem3	A	T	8: 80,613,336	I753F	probably damaging	Het
Gfra1	A	G	19: 58,300,312	S234P	probably damaging	Het
Gm5065	C	T	7: 5,359,441	R24C	probably benign	Het
Gtse1	T	A	15: 85,868,700	S339T	probably benign	Het
Hdgfl3	T	C	7: 81,900,358	N76S	probably benign	Het
Herc6	A	G	6: 57,581,104	E23G	probably benign	Het
Hid1	T	A	11: 115,354,719	H420L	probably damaging	Het
Igsf21	G	A	4: 140,027,738	T440I	probably benign	Het
Ikzf4	T	A	10: 128,632,582	H676L	unknown	Het
Il18r1	A	G	1: 40,480,980	E177G	probably benign	Het
Iqch	A	G	9: 63,508,317	Y558H	possibly damaging	Het
Kif11	A	T	19: 37,410,624	N752I	possibly damaging	Het
Kntc1	T	A	5: 123,786,956	C1111S	possibly damaging	Het
Krtap9-5	A	G	11: 99,948,974	K167R	unknown	Het
Lipe	G	T	7: 25,380,611	T704K	probably benign	Het
Lrrc32	A	T	7: 98,498,207	I65F	possibly damaging	Het
Mogs	C	T	6: 83,116,207	H179Y	probably damaging	Het
Mtnr1b	A	T	9: 15,863,294	Y156*	probably null	Het
Mtus1	T	C	8: 41,084,553	H42R	probably benign	Het
Mx2	T	A	16: 97,545,718	D128E	probably benign	Het
Myof	A	T	19: 37,951,491	L829*	probably null	Het
Naip6	T	C	13: 100,283,851	K1304E	probably benign	Het
Neo1	A	C	9: 58,884,543	S1307R	probably benign	Het
Nipbl	A	T	15: 8,330,295	D1475E	probably benign	Het
Nlrp9b	T	A	7: 20,023,950	F371I	probably damaging	Het
Nrip1	A	T	16: 76,291,450	M1073K	probably damaging	Het
Obox6	T	C	7: 15,833,938	N195D	probably damaging	Het
Olfr1451	C	T	19: 12,999,558	H191Y	probably benign	Het
Olfr206	A	T	16: 59,345,324	C126S	probably damaging	Het
Olfr347	T	A	2: 36,734,638	F106I	probably benign	Het
Olfr46	T	A	7: 140,610,178	I4K	probably benign	Het
Olfr488	C	A	7: 108,255,838	C100F	probably damaging	Het
Pax7	T	C	4: 139,784,569	K232E	probably benign	Het
Phldb3	C	T	7: 24,611,264		probably benign	Het
Pkd1l1	C	A	11: 8,965,148	V131L		Het
Ppp2r5a	A	G	1: 191,396,335	S28P	probably benign	Het
Prl8a1	A	G	13: 27,574,085	S214P	probably damaging	Het
Prmt2	A	T	10: 76,221,004	C228*	probably null	Het
Prpf8	C	T	11: 75,508,912	R2266*	probably null	Het
Rabl6	A	G	2: 25,584,141	S648P	unknown	Het
Ralgapa1	T	C	12: 55,712,672	K1022R	probably damaging	Het
Ralgapb	T	A	2: 158,443,355	D591E	probably benign	Het
Rprd1a	A	G	18: 24,506,832		probably null	Het
Rsu1	T	C	2: 13,216,875	R165G	probably damaging	Het
Samd8	A	G	14: 21,792,423	E334G	probably benign	Het
Scn9a	T	A	2: 66,534,217	Q804L	probably damaging	Het
Sez6	A	G	11: 77,973,885	D557G	probably benign	Het
Sgsm1	A	T	5: 113,279,635		probably null	Het
Slc17a3	T	A	13: 23,855,849	M290K		Het
Snd1	T	A	6: 28,531,450	V330E	probably benign	Het
Tceanc2	T	C	4: 107,165,655	K45R	probably damaging	Het
Tg	A	T	15: 66,696,588	I1375F	probably benign	Het
Tmem116	T	A	5: 121,495,061	I357K		Het
Tmem39a	G	T	16: 38,588,296	R407L	possibly damaging	Het
Tmem74	A	T	15: 43,867,365	M94K	probably benign	Het
Tpp1	C	T	7: 105,749,544	C226Y	probably damaging	Het
Trbv21	T	C	6: 41,202,927	I59T	not run	Het
Trim11	A	G	11: 58,978,637	D133G	probably benign	Het
Uaca	G	A	9: 60,846,000	V76I	probably damaging	Het
Ugt2a3	A	G	5: 87,327,680		probably null	Het
Wdr24	T	C	17: 25,826,127	Y279H	probably damaging	Het
Zbtb46	A	G	2: 181,423,719	S213P	probably benign	Het
Zdhhc13	C	A	7: 48,811,355	Y346*	probably null	Het
Zfp280b	A	T	10: 76,039,241	H318L	probably damaging	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20042725	missense	probably benign
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20030081	missense	probably damaging	1.00
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7514:Vmn2r104	UTSW	17	20029529	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8900:Vmn2r104	UTSW	17	20041662	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCATCAGGTCTTCAACTCAGAG -3'
(R):5'- TGGGAGTTATACCATGGCCTTC -3'

Sequencing Primer
(F):5'- TCAGGTCTTCAACTCAGAGATATTG -3'
(R):5'- GGAGTTATACCATGGCCTTCTTGTC -3'

Posted On

2019-10-07