Mutagenetix > Incidental Mutation

Incidental Mutation 'R4454:Mslnl'

329109

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

041714-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4454 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

100% (51/51)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010300C02Rik	T	C	1: 37,624,753	E163G	probably damaging	Het
AI481877	T	C	4: 59,092,383	D266G	possibly damaging	Het
Ap4e1	T	A	2: 127,047,141	F509I	probably damaging	Het
Asmt	A	T	X: 170,672,721	M19L	probably benign	Het
Atf6	C	T	1: 170,794,039	R471Q	probably damaging	Het
Atp4a	G	A	7: 30,720,225	R671Q	probably benign	Het
Baiap3	T	A	17: 25,249,536	D250V	probably damaging	Het
C2cd4d	T	A	3: 94,363,747	F107I	probably damaging	Het
Cdkn2d	C	G	9: 21,290,889	V21L	probably benign	Het
Cldn6	T	C	17: 23,681,086		probably null	Het
Cpa5	A	G	6: 30,626,324	N228S	possibly damaging	Het
Crocc	T	C	4: 141,020,405	S1478G	possibly damaging	Het
Csmd1	A	T	8: 15,945,011	C2675S	probably damaging	Het
Cthrc1	C	A	15: 39,077,013	Q4K	probably benign	Het
Ddo	A	T	10: 40,647,547	I178F	probably damaging	Het
Dmxl1	A	G	18: 49,893,332	T1836A	probably benign	Het
Dnah9	T	G	11: 66,147,389	Q107P	probably damaging	Het
Dusp26	A	G	8: 31,094,144	N93S	probably damaging	Het
Egr2	GAA	GA	10: 67,539,903		probably null	Het
Epha5	T	C	5: 84,156,444	I501V	probably damaging	Het
Eya1	C	T	1: 14,183,196	V519M	probably damaging	Het
Fam227b	T	A	2: 126,146,268		probably benign	Het
Fgd5	C	T	6: 91,989,186	S642F	probably damaging	Het
Fsip2	G	T	2: 82,990,776	A5618S	possibly damaging	Het
Gm12034	T	A	11: 20,446,476		noncoding transcript	Het
Gm13023	A	G	4: 143,792,824	S52G	probably benign	Het
Liph	T	C	16: 21,984,268	D17G	probably benign	Het
Mbd3	A	T	10: 80,393,983	L164H	probably damaging	Het
Med4	A	G	14: 73,518,062		probably benign	Het
Nav2	A	T	7: 49,548,544		probably null	Het
Olfr728	T	C	14: 50,140,496	I48V	probably benign	Het
Olfr904	C	A	9: 38,464,642	F200L	probably benign	Het
Pcdha11	G	A	18: 37,007,373	G685D	probably benign	Het
Pgc	A	G	17: 47,732,410	I228V	probably benign	Het
Rad51	C	T	2: 119,131,568	H199Y	probably damaging	Het
Robo2	A	T	16: 74,352,519		probably benign	Het
Sap130	C	T	18: 31,711,360	T861I	probably damaging	Het
Sh3tc2	A	T	18: 62,007,773	D1061V	probably damaging	Het
Snapc3	A	G	4: 83,418,759	E119G	probably damaging	Het
Sspo	G	A	6: 48,487,225	G3862D	probably benign	Het
Tbc1d16	G	A	11: 119,157,873	T318M	possibly damaging	Het
Thrb	T	A	14: 18,011,187	W188R	probably damaging	Het
Thsd1	T	C	8: 22,243,578	Y214H	probably damaging	Het
Tnfrsf13b	T	C	11: 61,141,438	V98A	probably benign	Het
Topbp1	T	C	9: 103,344,871	Y1314H	probably damaging	Het
Ttn	C	A	2: 76,785,806	V8271L	possibly damaging	Het
Ttn	T	C	2: 76,946,913	M1382V	probably benign	Het
Utrn	G	T	10: 12,727,840	Q599K	possibly damaging	Het
Zfp995	G	A	17: 21,879,951	T434I	probably benign	Het
Zfy1	G	T	Y: 725,518	T749K	possibly damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTGCCATCAGAAGGGAGGTTTG -3'
(R):5'- CACAGGTCTGAAGCACTGAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-07-21