Mutagenetix > Incidental Mutation

Incidental Mutation 'R4454:Mslnl'

329109

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

041714-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4454 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25955016-25967304 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25961908 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

100% (51/51)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ap4e1	T	A	2: 126,889,061 (GRCm39)	F509I	probably damaging	Het
Asmt	A	T	X: 169,106,456 (GRCm39)	M19L	probably benign	Het
Atf6	C	T	1: 170,621,608 (GRCm39)	R471Q	probably damaging	Het
Atp4a	G	A	7: 30,419,650 (GRCm39)	R671Q	probably benign	Het
Baiap3	T	A	17: 25,468,510 (GRCm39)	D250V	probably damaging	Het
C2cd4d	T	A	3: 94,271,054 (GRCm39)	F107I	probably damaging	Het
Cdkn2d	C	G	9: 21,202,185 (GRCm39)	V21L	probably benign	Het
Cldn6	T	C	17: 23,900,060 (GRCm39)		probably null	Het
Cpa5	A	G	6: 30,626,323 (GRCm39)	N228S	possibly damaging	Het
Cracdl	T	C	1: 37,663,834 (GRCm39)	E163G	probably damaging	Het
Crocc	T	C	4: 140,747,716 (GRCm39)	S1478G	possibly damaging	Het
Csmd1	A	T	8: 15,995,011 (GRCm39)	C2675S	probably damaging	Het
Cthrc1	C	A	15: 38,940,408 (GRCm39)	Q4K	probably benign	Het
Ddo	A	T	10: 40,523,543 (GRCm39)	I178F	probably damaging	Het
Dmxl1	A	G	18: 50,026,399 (GRCm39)	T1836A	probably benign	Het
Dnah9	T	G	11: 66,038,215 (GRCm39)	Q107P	probably damaging	Het
Dusp26	A	G	8: 31,584,172 (GRCm39)	N93S	probably damaging	Het
Egr2	GAA	GA	10: 67,375,733 (GRCm39)		probably null	Het
Epha5	T	C	5: 84,304,303 (GRCm39)	I501V	probably damaging	Het
Eya1	C	T	1: 14,253,420 (GRCm39)	V519M	probably damaging	Het
Fam227b	T	A	2: 125,988,188 (GRCm39)		probably benign	Het
Fgd5	C	T	6: 91,966,167 (GRCm39)	S642F	probably damaging	Het
Fsip2	G	T	2: 82,821,120 (GRCm39)	A5618S	possibly damaging	Het
Gm12034	T	A	11: 20,396,476 (GRCm39)		noncoding transcript	Het
Liph	T	C	16: 21,803,018 (GRCm39)	D17G	probably benign	Het
Mbd3	A	T	10: 80,229,817 (GRCm39)	L164H	probably damaging	Het
Med4	A	G	14: 73,755,502 (GRCm39)		probably benign	Het
Nav2	A	T	7: 49,198,292 (GRCm39)		probably null	Het
Or4k1	T	C	14: 50,377,953 (GRCm39)	I48V	probably benign	Het
Or8b1b	C	A	9: 38,375,938 (GRCm39)	F200L	probably benign	Het
Pcdha11	G	A	18: 37,140,426 (GRCm39)	G685D	probably benign	Het
Pgc	A	G	17: 48,043,335 (GRCm39)	I228V	probably benign	Het
Pramel25	A	G	4: 143,519,394 (GRCm39)	S52G	probably benign	Het
Rad51	C	T	2: 118,962,049 (GRCm39)	H199Y	probably damaging	Het
Robo2	A	T	16: 74,149,407 (GRCm39)		probably benign	Het
Sap130	C	T	18: 31,844,413 (GRCm39)	T861I	probably damaging	Het
Sh3tc2	A	T	18: 62,140,844 (GRCm39)	D1061V	probably damaging	Het
Shoc1	T	C	4: 59,092,383 (GRCm39)	D266G	possibly damaging	Het
Snapc3	A	G	4: 83,336,996 (GRCm39)	E119G	probably damaging	Het
Sspo	G	A	6: 48,464,159 (GRCm39)	G3862D	probably benign	Het
Tbc1d16	G	A	11: 119,048,699 (GRCm39)	T318M	possibly damaging	Het
Thrb	T	A	14: 18,011,187 (GRCm38)	W188R	probably damaging	Het
Thsd1	T	C	8: 22,733,594 (GRCm39)	Y214H	probably damaging	Het
Tnfrsf13b	T	C	11: 61,032,264 (GRCm39)	V98A	probably benign	Het
Topbp1	T	C	9: 103,222,070 (GRCm39)	Y1314H	probably damaging	Het
Ttn	C	A	2: 76,616,150 (GRCm39)	V8271L	possibly damaging	Het
Ttn	T	C	2: 76,777,257 (GRCm39)	M1382V	probably benign	Het
Utrn	G	T	10: 12,603,584 (GRCm39)	Q599K	possibly damaging	Het
Zfp995	G	A	17: 22,098,932 (GRCm39)	T434I	probably benign	Het
Zfy1	G	T	Y: 725,518 (GRCm39)	T749K	possibly damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,962,641 (GRCm39)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,963,749 (GRCm39)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,965,125 (GRCm39)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,966,972 (GRCm39)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,963,077 (GRCm39)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,963,051 (GRCm39)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,962,177 (GRCm39)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,961,939 (GRCm39)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,963,491 (GRCm39)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,963,943 (GRCm39)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,957,952 (GRCm39)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,957,942 (GRCm39)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,965,139 (GRCm39)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,962,133 (GRCm39)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,956,816 (GRCm39)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,965,749 (GRCm39)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,956,876 (GRCm39)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,963,531 (GRCm39)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,965,047 (GRCm39)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,962,186 (GRCm39)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,962,184 (GRCm39)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,955,895 (GRCm39)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,962,157 (GRCm39)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,965,751 (GRCm39)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,965,962 (GRCm39)	missense	probably benign
R8735:Mslnl	UTSW	17	25,964,062 (GRCm39)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,964,047 (GRCm39)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,961,694 (GRCm39)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,961,506 (GRCm39)	intron	probably benign
RF007:Mslnl	UTSW	17	25,962,202 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTGCCATCAGAAGGGAGGTTTG -3'
(R):5'- CACAGGTCTGAAGCACTGAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-07-21