Mutagenetix > Incidental Mutation

Incidental Mutation 'R4707:Usp34'

355347

Institutional Source

Beutler Lab

Gene Symbol

Usp34

Ensembl Gene

ENSMUSG00000056342

Gene Name

ubiquitin specific peptidase 34

Synonyms

Murr2, A530081C03Rik

MMRRC Submission

041955-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.803) question?

Stock #

R4707 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23256895-23440560 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23437215 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Serine at position 3326 (L3326S)

Ref Sequence

ENSEMBL: ENSMUSP00000137430 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020529] [ENSMUST00000109539] [ENSMUST00000129368] [ENSMUST00000180046] [ENSMUST00000147157]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000020529

SMART Domains

Protein: ENSMUSP00000020529
Gene: ENSMUSG00000020288

Domain	Start	End	E-Value	Type
Aha1_N	29	163	2.52e-57	SMART
Pfam:AHSA1	209	325	1.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000109539

SMART Domains

Protein: ENSMUSP00000105166
Gene: ENSMUSG00000020288

Domain	Start	End	E-Value	Type
Aha1_N	2	115	2.33e-38	SMART
Pfam:AHSA1	161	277	4.3e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122864

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124801

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125701

Predicted Effect

probably benign
Transcript: ENSMUST00000128372

SMART Domains

Protein: ENSMUSP00000121255
Gene: ENSMUSG00000020288

Domain	Start	End	E-Value	Type
Aha1_N	15	149	2.52e-57	SMART
Pfam:AHSA1	195	311	7.5e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000129368
AA Change: L233S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000121426
Gene: ENSMUSG00000056342
AA Change: L233S

Domain	Start	End	E-Value	Type
Blast:Drf_GBD	2	86	1e-19	BLAST
low complexity region	231	244	N/A	INTRINSIC
coiled coil region	259	281	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000180046
AA Change: L3326S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000137430
Gene: ENSMUSG00000056342
AA Change: L3326S

Domain	Start	End	E-Value	Type
low complexity region	469	480	N/A	INTRINSIC
low complexity region	510	524	N/A	INTRINSIC
low complexity region	571	590	N/A	INTRINSIC
coiled coil region	607	652	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	1187	1198	N/A	INTRINSIC
low complexity region	1379	1390	N/A	INTRINSIC
low complexity region	1498	1512	N/A	INTRINSIC
low complexity region	1731	1744	N/A	INTRINSIC
low complexity region	1792	1804	N/A	INTRINSIC
Pfam:UCH	1930	2273	2.3e-44	PFAM
Pfam:UCH_1	1931	2229	1.1e-22	PFAM
low complexity region	2522	2544	N/A	INTRINSIC
low complexity region	2652	2659	N/A	INTRINSIC
Blast:Drf_GBD	2923	3096	2e-53	BLAST
low complexity region	3324	3337	N/A	INTRINSIC
coiled coil region	3352	3374	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000137823
AA Change: L3345S

SMART Domains

Protein: ENSMUSP00000120747
Gene: ENSMUSG00000056342
AA Change: L3345S

Domain	Start	End	E-Value	Type
low complexity region	489	500	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	591	610	N/A	INTRINSIC
coiled coil region	626	671	N/A	INTRINSIC
low complexity region	827	842	N/A	INTRINSIC
low complexity region	1207	1218	N/A	INTRINSIC
low complexity region	1399	1410	N/A	INTRINSIC
low complexity region	1518	1532	N/A	INTRINSIC
low complexity region	1751	1764	N/A	INTRINSIC
low complexity region	1812	1824	N/A	INTRINSIC
Pfam:UCH	1950	2293	7.6e-44	PFAM
Pfam:UCH_1	1951	2249	3.6e-22	PFAM
low complexity region	2542	2564	N/A	INTRINSIC
low complexity region	2672	2679	N/A	INTRINSIC
Blast:Drf_GBD	2943	3116	3e-53	BLAST
low complexity region	3344	3357	N/A	INTRINSIC
coiled coil region	3371	3393	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153409

Predicted Effect

probably benign
Transcript: ENSMUST00000147157

SMART Domains

Protein: ENSMUSP00000121920
Gene: ENSMUSG00000020288

Domain	Start	End	E-Value	Type
Aha1_N	29	138	4.15e-26	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 115 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	A	G	17: 9,224,544 (GRCm39)	K450R	probably damaging	Het
Abcd2	T	C	15: 91,043,385 (GRCm39)	D601G	probably benign	Het
Abcf3	A	G	16: 20,367,808 (GRCm39)	K56E	possibly damaging	Het
Acaca	T	C	11: 84,203,680 (GRCm39)	V1477A	probably damaging	Het
Adamts20	G	A	15: 94,231,528 (GRCm39)	P887L	possibly damaging	Het
Ahnak	T	G	19: 8,994,099 (GRCm39)	S5128A	probably benign	Het
Ahsa2	C	A	11: 23,443,162 (GRCm39)	V197F	probably benign	Het
Ak9	C	A	10: 41,221,456 (GRCm39)	H402N	probably benign	Het
Alpk1	G	T	3: 127,481,241 (GRCm39)	N175K	possibly damaging	Het
Als2	A	G	1: 59,254,472 (GRCm39)	V295A	probably benign	Het
Ankrd16	A	G	2: 11,783,608 (GRCm39)	D70G	probably damaging	Het
Apob	A	T	12: 8,056,205 (GRCm39)	K1562N	probably damaging	Het
Arfgap2	C	A	2: 91,100,316 (GRCm39)	S250R	probably damaging	Het
Arhgef10l	C	T	4: 140,264,194 (GRCm39)	M671I	possibly damaging	Het
Atrnl1	A	G	19: 57,617,590 (GRCm39)	I122V	probably damaging	Het
B4galnt2	C	T	11: 95,766,923 (GRCm39)		probably null	Het
C8a	A	G	4: 104,713,618 (GRCm39)	Y171H	probably damaging	Het
Cacnb4	A	G	2: 52,364,927 (GRCm39)	V112A	probably benign	Het
Capn9	G	T	8: 125,340,195 (GRCm39)	C566F	possibly damaging	Het
Ccdc88a	T	A	11: 29,397,956 (GRCm39)	S230T	probably benign	Het
Cd300c2	A	T	11: 114,887,811 (GRCm39)	F197Y	probably benign	Het
Chd5	A	G	4: 152,445,039 (GRCm39)	Y340C	probably damaging	Het
Chrd	A	T	16: 20,557,558 (GRCm39)	I726F	possibly damaging	Het
Chrna10	G	A	7: 101,762,426 (GRCm39)	P255S	possibly damaging	Het
Clasp1	T	A	1: 118,470,927 (GRCm39)	Y197*	probably null	Het
Cyp2c69	C	G	19: 39,837,852 (GRCm39)	G410A	probably benign	Het
Cyp2e1	G	A	7: 140,343,821 (GRCm39)	V20I	possibly damaging	Het
Dapp1	T	C	3: 137,638,928 (GRCm39)	D225G	probably benign	Het
Dnah5	A	T	15: 28,372,521 (GRCm39)	D2924V	probably damaging	Het
Efcab12	A	T	6: 115,791,510 (GRCm39)	L554Q	possibly damaging	Het
Emsy	T	C	7: 98,246,311 (GRCm39)	T228A	possibly damaging	Het
Evc2	G	A	5: 37,579,204 (GRCm39)	V1106I	probably benign	Het
Exoc8	G	T	8: 125,624,209 (GRCm39)	Q53K	possibly damaging	Het
Fbn2	A	T	18: 58,189,344 (GRCm39)	V1594D	probably damaging	Het
Fer1l4	T	C	2: 155,887,543 (GRCm39)	Y551C	possibly damaging	Het
Fhip1a	G	T	3: 85,595,877 (GRCm39)	T115K	probably damaging	Het
Fmnl3	A	G	15: 99,221,362 (GRCm39)	M481T	probably benign	Het
Fras1	A	G	5: 96,883,097 (GRCm39)	N2543S	probably damaging	Het
Fsd2	T	C	7: 81,209,428 (GRCm39)	D138G	probably damaging	Het
Gda	C	T	19: 21,405,992 (GRCm39)	V5I	probably benign	Het
Glt8d2	A	T	10: 82,496,583 (GRCm39)	D158E	probably damaging	Het
Gpx6	C	T	13: 21,496,434 (GRCm39)	Q3*	probably null	Het
Greb1l	A	G	18: 10,532,922 (GRCm39)	M830V	probably benign	Het
Hnrnpul1	A	C	7: 25,426,258 (GRCm39)	V531G	probably damaging	Het
Ifi206	A	T	1: 173,308,432 (GRCm39)	H521Q	probably benign	Het
Igsf3	G	C	3: 101,365,410 (GRCm39)	R1127P	probably benign	Het
Il1rl1	A	G	1: 40,489,348 (GRCm39)	R367G	probably damaging	Het
Islr	T	C	9: 58,064,970 (GRCm39)	D179G	possibly damaging	Het
Jcad	G	T	18: 4,649,338 (GRCm39)	E70*	probably null	Het
Kcnd2	T	C	6: 21,723,211 (GRCm39)	I467T	probably benign	Het
Lrrc27	A	G	7: 138,822,614 (GRCm39)	T502A	probably benign	Het
Lrrtm3	T	A	10: 63,923,781 (GRCm39)	H462L	probably benign	Het
Mbd5	T	C	2: 49,140,168 (GRCm39)	L44S	probably damaging	Het
Mccc1	C	A	3: 36,030,022 (GRCm39)	M429I	probably damaging	Het
Mgam	A	T	6: 40,691,566 (GRCm39)		probably null	Het
Mipep	T	A	14: 61,109,552 (GRCm39)	I643N	probably damaging	Het
Mmrn1	A	T	6: 60,965,457 (GRCm39)	I1162L	probably benign	Het
Mrc2	G	A	11: 105,239,257 (GRCm39)		probably null	Het
Mug1	G	A	6: 121,861,600 (GRCm39)	C1354Y	probably damaging	Het
Nbeal2	A	T	9: 110,461,123 (GRCm39)	S1647T	probably benign	Het
Ndufaf2	C	G	13: 108,189,314 (GRCm39)	A145P	probably damaging	Het
Nedd9	A	T	13: 41,492,051 (GRCm39)		probably null	Het
Nr4a2	T	A	2: 57,002,105 (GRCm39)	H116L	probably benign	Het
Nwd2	A	G	5: 63,951,665 (GRCm39)	Y232C	probably damaging	Het
Odf4	A	G	11: 68,817,514 (GRCm39)	L58P	probably damaging	Het
Or2a20	T	A	6: 43,193,883 (GRCm39)	V12D	probably benign	Het
Or4a27	T	A	2: 88,559,056 (GRCm39)	M296L	possibly damaging	Het
Or4c116	A	T	2: 88,942,576 (GRCm39)	F93L	probably damaging	Het
Or4g17	T	A	2: 111,209,990 (GRCm39)	L215H	probably damaging	Het
Or7e165	C	T	9: 19,694,596 (GRCm39)	H18Y	probably damaging	Het
Or8g24	A	G	9: 38,989,558 (GRCm39)	V161A	probably benign	Het
Or8s5	G	T	15: 98,238,659 (GRCm39)	H70Q	possibly damaging	Het
Orc6	T	A	8: 86,029,579 (GRCm39)	I41K	probably damaging	Het
Pikfyve	A	G	1: 65,307,005 (GRCm39)	T1798A	probably benign	Het
Prdx6b	T	C	2: 80,123,404 (GRCm39)	L71P	probably damaging	Het
Prmt2	A	G	10: 76,062,055 (GRCm39)	I50T	probably damaging	Het
Ptdss1	T	C	13: 67,143,482 (GRCm39)		probably null	Het
Pygl	T	C	12: 70,254,532 (GRCm39)	T138A	possibly damaging	Het
Rab3c	T	C	13: 110,198,434 (GRCm39)	E198G	probably benign	Het
Rbl2	A	G	8: 91,812,196 (GRCm39)	Y255C	probably damaging	Het
Rev3l	T	A	10: 39,699,393 (GRCm39)	S1297T	probably damaging	Het
Rgma	C	A	7: 73,067,564 (GRCm39)	T367K	probably damaging	Het
Rps6ka5	C	T	12: 100,564,144 (GRCm39)		probably null	Het
Rslcan18	A	T	13: 67,246,590 (GRCm39)	C217S	probably damaging	Het
Rtp3	A	G	9: 110,815,279 (GRCm39)		probably benign	Het
Ryr1	A	T	7: 28,745,087 (GRCm39)	N3848K	probably damaging	Het
Sema6a	T	A	18: 47,381,779 (GRCm39)	T923S	probably benign	Het
Serpinb6d	A	T	13: 33,855,336 (GRCm39)	T337S	possibly damaging	Het
Sf3b1	G	A	1: 55,029,666 (GRCm39)	T1112M	probably damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Sidt1	A	T	16: 44,090,221 (GRCm39)	Y369*	probably null	Het
Slc16a6	A	G	11: 109,354,193 (GRCm39)	S59P	probably benign	Het
Slc25a17	A	C	15: 81,211,527 (GRCm39)	L163W	probably damaging	Het
Slc44a3	T	C	3: 121,320,723 (GRCm39)	T93A	possibly damaging	Het
Sptbn1	T	C	11: 30,087,197 (GRCm39)	T1081A	possibly damaging	Het
Sptbn4	T	C	7: 27,116,431 (GRCm39)	D456G	probably benign	Het
Sycp1	C	A	3: 102,760,805 (GRCm39)	A703S	possibly damaging	Het
Tas2r118	T	A	6: 23,969,225 (GRCm39)	M279L	probably benign	Het
Tc2n	T	G	12: 101,660,832 (GRCm39)	Q133H	probably benign	Het
Tctn1	A	G	5: 122,399,468 (GRCm39)		probably null	Het
Tenm4	A	C	7: 96,423,253 (GRCm39)	K683Q	probably damaging	Het
Tex10	A	T	4: 48,468,984 (GRCm39)	S64T	probably benign	Het
Tex15	A	G	8: 34,072,525 (GRCm39)	T2691A	probably benign	Het
Tmem175	A	T	5: 108,790,016 (GRCm39)	T123S	probably damaging	Het
Tsc1	C	A	2: 28,562,419 (GRCm39)	S348R	probably damaging	Het
Ttll4	A	T	1: 74,718,166 (GRCm39)	T6S	possibly damaging	Het
Ttll8	A	T	15: 88,801,293 (GRCm39)	I465N	probably damaging	Het
Ubr3	A	G	2: 69,768,714 (GRCm39)		probably benign	Het
Ugdh	A	T	5: 65,580,695 (GRCm39)		probably null	Het
Vmn2r125	A	T	4: 156,702,276 (GRCm39)	I21F	probably damaging	Het
Vps9d1	G	A	8: 123,975,351 (GRCm39)		probably benign	Het
Xab2	G	A	8: 3,668,117 (GRCm39)	R154C	possibly damaging	Het
Zbtb12	T	C	17: 35,114,475 (GRCm39)	S87P	probably damaging	Het
Zc3h12a	A	G	4: 125,014,686 (GRCm39)	M266T	probably damaging	Het
Zfp108	G	A	7: 23,959,837 (GRCm39)	A143T	probably benign	Het

Other mutations in Usp34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL00477:Usp34	APN	11	23,418,879 (GRCm39)	missense	probably damaging	0.99
IGL01307:Usp34	APN	11	23,367,676 (GRCm39)	missense	probably damaging	0.99
IGL01313:Usp34	APN	11	23,423,206 (GRCm39)	missense	probably damaging	1.00
IGL01794:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01826:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01827:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01830:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01867:Usp34	APN	11	23,334,411 (GRCm39)	missense	possibly damaging	0.77
IGL01939:Usp34	APN	11	23,295,141 (GRCm39)	splice site	probably benign
IGL01977:Usp34	APN	11	23,402,661 (GRCm39)	missense	probably damaging	1.00
IGL01985:Usp34	APN	11	23,402,565 (GRCm39)	missense	probably damaging	1.00
IGL02011:Usp34	APN	11	23,421,554 (GRCm39)	missense	probably damaging	0.99
IGL02302:Usp34	APN	11	23,417,243 (GRCm39)	missense	possibly damaging	0.91
IGL02423:Usp34	APN	11	23,304,900 (GRCm39)	missense	probably benign	0.11
IGL02491:Usp34	APN	11	23,382,630 (GRCm39)	missense	probably damaging	0.98
IGL02532:Usp34	APN	11	23,320,291 (GRCm39)	missense	probably damaging	0.99
IGL02561:Usp34	APN	11	23,301,652 (GRCm39)	missense	probably benign	0.09
IGL02706:Usp34	APN	11	23,338,659 (GRCm39)	splice site	probably benign
IGL02891:Usp34	APN	11	23,437,166 (GRCm39)	missense	probably benign	0.09
IGL03079:Usp34	APN	11	23,382,247 (GRCm39)	missense	possibly damaging	0.48
IGL03089:Usp34	APN	11	23,396,958 (GRCm39)	missense	possibly damaging	0.84
IGL03175:Usp34	APN	11	23,438,686 (GRCm39)	missense	probably benign
IGL03256:Usp34	APN	11	23,370,090 (GRCm39)	nonsense	probably null
IGL03280:Usp34	APN	11	23,304,897 (GRCm39)	missense	probably damaging	1.00
IGL03289:Usp34	APN	11	23,343,818 (GRCm39)	missense	possibly damaging	0.94
IGL03408:Usp34	APN	11	23,396,957 (GRCm39)	missense	possibly damaging	0.92
Chub	UTSW	11	23,414,686 (GRCm39)	missense	probably damaging	0.99
Cicione	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R5571_Usp34_680	UTSW	11	23,407,975 (GRCm39)	missense	probably damaging	0.99
R5713_Usp34_003	UTSW	11	23,293,515 (GRCm39)	missense	possibly damaging	0.94
Roebuck	UTSW	11	23,436,810 (GRCm39)	splice site	probably benign
stoat	UTSW	11	23,437,203 (GRCm39)	missense
tunnelvision	UTSW	11	23,396,968 (GRCm39)	missense
I2288:Usp34	UTSW	11	23,382,473 (GRCm39)	splice site	probably benign
R0047:Usp34	UTSW	11	23,414,403 (GRCm39)	missense	probably benign	0.34
R0047:Usp34	UTSW	11	23,414,403 (GRCm39)	missense	probably benign	0.34
R0099:Usp34	UTSW	11	23,313,111 (GRCm39)	missense	probably damaging	1.00
R0240:Usp34	UTSW	11	23,383,206 (GRCm39)	missense	probably damaging	0.99
R0240:Usp34	UTSW	11	23,383,206 (GRCm39)	missense	probably damaging	0.99
R0403:Usp34	UTSW	11	23,283,838 (GRCm39)	missense	possibly damaging	0.82
R0432:Usp34	UTSW	11	23,351,505 (GRCm39)	missense	probably damaging	0.99
R0446:Usp34	UTSW	11	23,417,207 (GRCm39)	missense	probably damaging	0.97
R0455:Usp34	UTSW	11	23,396,741 (GRCm39)	splice site	probably benign
R0470:Usp34	UTSW	11	23,386,001 (GRCm39)	missense	possibly damaging	0.94
R0472:Usp34	UTSW	11	23,334,509 (GRCm39)	splice site	probably benign
R0512:Usp34	UTSW	11	23,401,997 (GRCm39)	missense	probably benign	0.04
R0557:Usp34	UTSW	11	23,353,848 (GRCm39)	missense	probably damaging	0.98
R0562:Usp34	UTSW	11	23,382,406 (GRCm39)	splice site	probably benign
R0656:Usp34	UTSW	11	23,422,967 (GRCm39)	missense	probably damaging	0.99
R0693:Usp34	UTSW	11	23,402,637 (GRCm39)	missense	probably damaging	0.97
R0739:Usp34	UTSW	11	23,417,243 (GRCm39)	missense	possibly damaging	0.91
R1061:Usp34	UTSW	11	23,334,420 (GRCm39)	missense	possibly damaging	0.51
R1078:Usp34	UTSW	11	23,383,175 (GRCm39)	splice site	probably benign
R1223:Usp34	UTSW	11	23,396,464 (GRCm39)	splice site	probably null
R1295:Usp34	UTSW	11	23,334,477 (GRCm39)	missense	probably damaging	1.00
R1430:Usp34	UTSW	11	23,409,151 (GRCm39)	missense	probably damaging	0.97
R1445:Usp34	UTSW	11	23,301,629 (GRCm39)	missense	probably damaging	0.99
R1468:Usp34	UTSW	11	23,391,171 (GRCm39)	missense	probably damaging	1.00
R1468:Usp34	UTSW	11	23,391,171 (GRCm39)	missense	probably damaging	1.00
R1471:Usp34	UTSW	11	23,438,862 (GRCm39)	missense	probably benign	0.20
R1475:Usp34	UTSW	11	23,423,253 (GRCm39)	missense	probably damaging	0.99
R1628:Usp34	UTSW	11	23,438,725 (GRCm39)	missense	probably damaging	1.00
R1631:Usp34	UTSW	11	23,410,651 (GRCm39)	missense	probably damaging	0.99
R1655:Usp34	UTSW	11	23,325,051 (GRCm39)	missense	probably benign	0.05
R1741:Usp34	UTSW	11	23,314,103 (GRCm39)	missense	probably benign	0.00
R1854:Usp34	UTSW	11	23,376,153 (GRCm39)	missense	probably benign	0.24
R1867:Usp34	UTSW	11	23,311,593 (GRCm39)	missense	possibly damaging	0.82
R1869:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1870:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1871:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1967:Usp34	UTSW	11	23,314,503 (GRCm39)	missense	probably benign	0.01
R2051:Usp34	UTSW	11	23,414,468 (GRCm39)	missense	probably damaging	0.97
R2132:Usp34	UTSW	11	23,414,556 (GRCm39)	missense	possibly damaging	0.95
R2156:Usp34	UTSW	11	23,332,602 (GRCm39)	missense	probably damaging	0.98
R2205:Usp34	UTSW	11	23,335,147 (GRCm39)	missense	probably damaging	0.97
R2342:Usp34	UTSW	11	23,353,599 (GRCm39)	missense	possibly damaging	0.46
R3431:Usp34	UTSW	11	23,320,466 (GRCm39)	missense	possibly damaging	0.95
R3812:Usp34	UTSW	11	23,414,517 (GRCm39)	missense	possibly damaging	0.94
R3872:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3873:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3874:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3875:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3925:Usp34	UTSW	11	23,293,640 (GRCm39)	missense	probably benign	0.28
R3972:Usp34	UTSW	11	23,407,803 (GRCm39)	missense	probably damaging	1.00
R4018:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R4042:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R4155:Usp34	UTSW	11	23,367,676 (GRCm39)	missense	probably damaging	0.99
R4197:Usp34	UTSW	11	23,394,189 (GRCm39)	missense	probably damaging	0.98
R4352:Usp34	UTSW	11	23,270,727 (GRCm39)	missense	possibly damaging	0.73
R4379:Usp34	UTSW	11	23,334,499 (GRCm39)	missense	possibly damaging	0.52
R4444:Usp34	UTSW	11	23,385,998 (GRCm39)	missense	probably damaging	0.98
R4475:Usp34	UTSW	11	23,407,975 (GRCm39)	missense	possibly damaging	0.95
R4501:Usp34	UTSW	11	23,351,529 (GRCm39)	missense	probably damaging	1.00
R4527:Usp34	UTSW	11	23,371,257 (GRCm39)	missense	possibly damaging	0.57
R4603:Usp34	UTSW	11	23,414,633 (GRCm39)	missense	probably damaging	0.97
R4612:Usp34	UTSW	11	23,382,268 (GRCm39)	missense	probably damaging	0.99
R4673:Usp34	UTSW	11	23,314,480 (GRCm39)	small deletion	probably benign
R4736:Usp34	UTSW	11	23,343,749 (GRCm39)	splice site	probably null
R4867:Usp34	UTSW	11	23,401,999 (GRCm39)	missense	probably benign	0.28
R4879:Usp34	UTSW	11	23,323,410 (GRCm39)	missense	possibly damaging	0.94
R4977:Usp34	UTSW	11	23,438,982 (GRCm39)	missense	probably damaging	1.00
R5004:Usp34	UTSW	11	23,414,586 (GRCm39)	missense	probably damaging	1.00
R5057:Usp34	UTSW	11	23,408,086 (GRCm39)	intron	probably benign
R5068:Usp34	UTSW	11	23,410,665 (GRCm39)	missense	possibly damaging	0.94
R5304:Usp34	UTSW	11	23,293,616 (GRCm39)	missense	probably damaging	1.00
R5320:Usp34	UTSW	11	23,283,739 (GRCm39)	missense	probably benign
R5327:Usp34	UTSW	11	23,418,846 (GRCm39)	missense	probably damaging	1.00
R5328:Usp34	UTSW	11	23,438,659 (GRCm39)	missense	probably benign	0.04
R5328:Usp34	UTSW	11	23,414,616 (GRCm39)	missense	probably benign	0.01
R5390:Usp34	UTSW	11	23,394,202 (GRCm39)	critical splice donor site	probably null
R5434:Usp34	UTSW	11	23,362,271 (GRCm39)	missense	probably damaging	0.99
R5523:Usp34	UTSW	11	23,299,198 (GRCm39)	missense	probably benign	0.39
R5567:Usp34	UTSW	11	23,438,336 (GRCm39)	missense	probably damaging	0.97
R5571:Usp34	UTSW	11	23,407,975 (GRCm39)	missense	probably damaging	0.99
R5645:Usp34	UTSW	11	23,325,024 (GRCm39)	missense	possibly damaging	0.86
R5713:Usp34	UTSW	11	23,293,515 (GRCm39)	missense	possibly damaging	0.94
R5719:Usp34	UTSW	11	23,304,846 (GRCm39)	missense	probably benign	0.00
R5813:Usp34	UTSW	11	23,371,340 (GRCm39)	missense	probably benign	0.38
R5921:Usp34	UTSW	11	23,414,686 (GRCm39)	missense	probably damaging	0.99
R5928:Usp34	UTSW	11	23,386,040 (GRCm39)	missense	probably damaging	0.98
R5944:Usp34	UTSW	11	23,313,089 (GRCm39)	missense	probably damaging	1.00
R6198:Usp34	UTSW	11	23,434,127 (GRCm39)	missense	probably damaging	1.00
R6229:Usp34	UTSW	11	23,396,778 (GRCm39)	missense	probably damaging	0.99
R6306:Usp34	UTSW	11	23,362,260 (GRCm39)	missense	possibly damaging	0.94
R6320:Usp34	UTSW	11	23,402,520 (GRCm39)	missense	probably damaging	0.98
R6341:Usp34	UTSW	11	23,331,353 (GRCm39)	missense	probably damaging	0.97
R6374:Usp34	UTSW	11	23,388,914 (GRCm39)	missense	probably damaging	1.00
R6398:Usp34	UTSW	11	23,438,666 (GRCm39)	missense	probably benign
R6438:Usp34	UTSW	11	23,314,266 (GRCm39)	missense	probably benign	0.02
R6668:Usp34	UTSW	11	23,410,659 (GRCm39)	missense	probably damaging	0.97
R6700:Usp34	UTSW	11	23,389,011 (GRCm39)	missense	probably damaging	1.00
R6783:Usp34	UTSW	11	23,362,318 (GRCm39)	missense	probably damaging	1.00
R6821:Usp34	UTSW	11	23,317,491 (GRCm39)	missense	possibly damaging	0.79
R6855:Usp34	UTSW	11	23,402,569 (GRCm39)	missense	possibly damaging	0.94
R6916:Usp34	UTSW	11	23,408,023 (GRCm39)	missense	probably damaging	0.98
R7020:Usp34	UTSW	11	23,343,954 (GRCm39)	missense	probably benign	0.05
R7026:Usp34	UTSW	11	23,311,622 (GRCm39)	missense	probably damaging	1.00
R7085:Usp34	UTSW	11	23,313,097 (GRCm39)	missense
R7101:Usp34	UTSW	11	23,376,183 (GRCm39)	missense
R7168:Usp34	UTSW	11	23,414,585 (GRCm39)	missense
R7192:Usp34	UTSW	11	23,410,571 (GRCm39)	missense
R7264:Usp34	UTSW	11	23,283,566 (GRCm39)	missense	probably benign	0.00
R7325:Usp34	UTSW	11	23,369,052 (GRCm39)	missense
R7343:Usp34	UTSW	11	23,438,868 (GRCm39)	missense
R7358:Usp34	UTSW	11	23,311,683 (GRCm39)	missense	probably damaging	0.99
R7369:Usp34	UTSW	11	23,382,361 (GRCm39)	missense
R7389:Usp34	UTSW	11	23,295,200 (GRCm39)	missense
R7459:Usp34	UTSW	11	23,314,458 (GRCm39)	missense	possibly damaging	0.53
R7517:Usp34	UTSW	11	23,396,968 (GRCm39)	missense
R7729:Usp34	UTSW	11	23,399,268 (GRCm39)	missense
R7777:Usp34	UTSW	11	23,332,638 (GRCm39)	missense
R7810:Usp34	UTSW	11	23,362,314 (GRCm39)	missense
R7836:Usp34	UTSW	11	23,396,614 (GRCm39)	missense
R7862:Usp34	UTSW	11	23,414,718 (GRCm39)	missense
R7993:Usp34	UTSW	11	23,327,622 (GRCm39)	missense
R8050:Usp34	UTSW	11	23,396,787 (GRCm39)	missense
R8054:Usp34	UTSW	11	23,311,295 (GRCm39)	missense
R8239:Usp34	UTSW	11	23,396,750 (GRCm39)	missense
R8266:Usp34	UTSW	11	23,436,810 (GRCm39)	splice site	probably benign
R8347:Usp34	UTSW	11	23,362,345 (GRCm39)	missense
R8409:Usp34	UTSW	11	23,407,811 (GRCm39)	missense
R8692:Usp34	UTSW	11	23,379,325 (GRCm39)	missense
R8694:Usp34	UTSW	11	23,434,161 (GRCm39)	missense
R8734:Usp34	UTSW	11	23,394,184 (GRCm39)	missense
R8806:Usp34	UTSW	11	23,434,143 (GRCm39)	missense
R8914:Usp34	UTSW	11	23,293,604 (GRCm39)	missense
R8987:Usp34	UTSW	11	23,414,267 (GRCm39)	missense
R9013:Usp34	UTSW	11	23,320,302 (GRCm39)	missense
R9108:Usp34	UTSW	11	23,320,528 (GRCm39)	missense
R9264:Usp34	UTSW	11	23,439,064 (GRCm39)	missense
R9301:Usp34	UTSW	11	23,422,951 (GRCm39)	missense
R9375:Usp34	UTSW	11	23,437,203 (GRCm39)	missense
R9385:Usp34	UTSW	11	23,399,223 (GRCm39)	missense
R9500:Usp34	UTSW	11	23,331,337 (GRCm39)	missense	probably damaging	0.99
R9566:Usp34	UTSW	11	23,317,529 (GRCm39)	missense
R9629:Usp34	UTSW	11	23,314,364 (GRCm39)	missense
R9679:Usp34	UTSW	11	23,394,369 (GRCm39)	missense
R9680:Usp34	UTSW	11	23,317,385 (GRCm39)	missense	possibly damaging	0.94
R9686:Usp34	UTSW	11	23,424,351 (GRCm39)	missense
R9752:Usp34	UTSW	11	23,409,182 (GRCm39)	missense	probably benign	0.11
X0023:Usp34	UTSW	11	23,325,028 (GRCm39)	missense	possibly damaging	0.73
X0057:Usp34	UTSW	11	23,407,824 (GRCm39)	missense	possibly damaging	0.86
Z1176:Usp34	UTSW	11	23,423,221 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCTCTTCTGAAATGTCATTTCTGC -3'
(R):5'- TCAGTCTTACGTTCTCAGTACTGG -3'

Sequencing Primer
(F):5'- AGGATTTGTTTTGTGCATGTACAC -3'
(R):5'- CGTTCTCAGTACTGGAAAATATGTGC -3'

Posted On

2015-10-21