Mutagenetix > Incidental Mutation

Incidental Mutation 'R7254:Ralgapa1'

564153

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, 2310003F20Rik, Tulip1

MMRRC Submission

045315-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.755) question?

Stock #

R7254 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

55602896-55821167 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 55695193 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 1310 (H1310L)

Ref Sequence

ENSEMBL: ENSMUSP00000082503 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably damaging
Transcript: ENSMUST00000085385
AA Change: H1310L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: H1310L

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000110687
AA Change: H1310L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: H1310L

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000219432
AA Change: H1357L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000219566
AA Change: H41L

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Predicted Effect

probably damaging
Transcript: ENSMUST00000220367
AA Change: H1310L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226244
AA Change: H1766L

PolyPhen 2 Score 0.663 (Sensitivity: 0.86; Specificity: 0.91)

Meta Mutation Damage Score

0.3943

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	T	C	13: 59,741,976 (GRCm38)	I677V	probably benign	Het
4931406B18Rik	A	T	7: 43,498,199 (GRCm38)	D249E	probably damaging	Het
Acacb	A	C	5: 114,209,751 (GRCm38)		probably null	Het
Adnp	A	G	2: 168,183,998 (GRCm38)	V459A	probably damaging	Het
Arid3b	T	C	9: 57,796,754 (GRCm38)	K304E	probably damaging	Het
Ash1l	G	T	3: 89,070,509 (GRCm38)	R2713L	probably damaging	Het
Bmpr1a	A	C	14: 34,414,763 (GRCm38)	D490E	probably benign	Het
Cacna1g	A	T	11: 94,432,567 (GRCm38)	C1270*	probably null	Het
Cep295nl	G	T	11: 118,333,040 (GRCm38)	P326Q	probably damaging	Het
Cfap100	T	C	6: 90,406,061 (GRCm38)	I377V	unknown	Het
Creb1	C	T	1: 64,576,277 (GRCm38)	Q223*	probably null	Het
Ctsc	G	A	7: 88,309,559 (GRCm38)	G349D	probably damaging	Het
Ddx41	G	A	13: 55,533,956 (GRCm38)	R311*	probably null	Het
Dse	T	A	10: 34,184,148 (GRCm38)		probably benign	Het
Eef2k	C	T	7: 120,889,265 (GRCm38)	H458Y	probably benign	Het
Gipr	A	T	7: 19,163,613 (GRCm38)	V90E	probably damaging	Het
Gm14412	A	T	2: 177,317,396 (GRCm38)	D22E	probably damaging	Het
Gm17018	A	G	19: 45,577,034 (GRCm38)	Q149R	probably benign	Het
Gm21886	A	T	18: 80,089,735 (GRCm38)	C69*	probably null	Het
Gm5114	G	A	7: 39,408,966 (GRCm38)	L410F	probably benign	Het
Gmip	T	A	8: 69,816,468 (GRCm38)		probably null	Het
Gtf2f1	T	C	17: 57,007,101 (GRCm38)	T128A	possibly damaging	Het
Hnrnpr	A	G	4: 136,332,575 (GRCm38)	E330G	possibly damaging	Het
Hoxd9	T	A	2: 74,698,374 (GRCm38)	W107R	probably damaging	Het
Iars	T	C	13: 49,723,078 (GRCm38)		probably null	Het
Idh2	TCCCAGGGCC	TCC	7: 80,098,331 (GRCm38)		probably null	Het
Ifi213	G	T	1: 173,593,963 (GRCm38)	P120Q	probably damaging	Het
Il6	A	T	5: 30,014,908 (GRCm38)	Q94L	probably benign	Het
Kcnab1	A	G	3: 65,319,487 (GRCm38)	S196G	probably benign	Het
Kcnv1	C	T	15: 45,113,208 (GRCm38)	V228I	probably benign	Het
Lars2	A	G	9: 123,454,963 (GRCm38)	T739A	possibly damaging	Het
Med13	T	C	11: 86,319,835 (GRCm38)	S494G	probably benign	Het
Mtrf1	GCCTTC	GC	14: 79,423,491 (GRCm38)		probably null	Het
Myh9	T	G	15: 77,765,824 (GRCm38)	Q1646P	probably damaging	Het
Nif3l1	T	A	1: 58,450,466 (GRCm38)	S171R	probably benign	Het
Olfr143	T	C	9: 38,253,423 (GRCm38)	M2T	probably benign	Het
Olfr1467	A	T	19: 13,365,111 (GRCm38)	D161V	probably benign	Het
Olfr376	A	G	11: 73,375,375 (GRCm38)	I212V	probably benign	Het
Olfr398	A	G	11: 73,983,777 (GRCm38)	V277A	probably benign	Het
Olfr564	A	G	7: 102,803,558 (GRCm38)	T27A	probably benign	Het
Olfr702	A	G	7: 106,823,570 (GRCm38)	*319Q	probably null	Het
Olfr785	T	C	10: 129,409,780 (GRCm38)	V138A	probably benign	Het
Pak7	A	T	2: 136,116,764 (GRCm38)	S135T	possibly damaging	Het
Prr29	A	T	11: 106,374,858 (GRCm38)	M1L	probably damaging	Het
Ptpn13	T	A	5: 103,594,636 (GRCm38)	V2407E	probably damaging	Het
Raph1	T	C	1: 60,499,608 (GRCm38)	S393G	unknown	Het
Rgl2	T	C	17: 33,934,990 (GRCm38)	F457L	possibly damaging	Het
Ror2	A	G	13: 53,118,720 (GRCm38)	I303T	possibly damaging	Het
Runx2	G	A	17: 44,814,192 (GRCm38)	P80L	probably damaging	Het
Scn10a	C	T	9: 119,618,855 (GRCm38)	D1378N	probably damaging	Het
Serpinc1	T	A	1: 160,993,618 (GRCm38)	C91S	probably benign	Het
Sorbs1	G	C	19: 40,376,800 (GRCm38)	R180G	probably benign	Het
Tada1	T	A	1: 166,388,648 (GRCm38)	C139*	probably null	Het
Tbr1	T	A	2: 61,806,042 (GRCm38)	V254E	probably damaging	Het
Timd4	A	T	11: 46,843,189 (GRCm38)	I340F	probably benign	Het
Tubb2a	T	C	13: 34,074,532 (GRCm38)	Y425C	probably damaging	Het
Wdr60	A	G	12: 116,262,585 (GRCm38)		probably benign	Het
Zfp292	A	T	4: 34,819,476 (GRCm38)	M287K	probably damaging	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,722,773 (GRCm38)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,747,185 (GRCm38)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,702,452 (GRCm38)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,709,575 (GRCm38)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,642,359 (GRCm38)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,642,348 (GRCm38)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,777,316 (GRCm38)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,719,657 (GRCm38)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,642,477 (GRCm38)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,708,077 (GRCm38)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,642,449 (GRCm38)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,712,665 (GRCm38)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,673,507 (GRCm38)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,676,417 (GRCm38)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,717,069 (GRCm38)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,757,951 (GRCm38)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
bantam	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
wickedwarlock	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,795,653 (GRCm38)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,695,157 (GRCm38)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,786,263 (GRCm38)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,739,505 (GRCm38)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,676,569 (GRCm38)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,677,038 (GRCm38)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,708,067 (GRCm38)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,689,791 (GRCm38)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,676,765 (GRCm38)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,782,885 (GRCm38)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,795,698 (GRCm38)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,665,663 (GRCm38)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,676,581 (GRCm38)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,762,661 (GRCm38)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,707,978 (GRCm38)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,676,926 (GRCm38)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,741,480 (GRCm38)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,684,524 (GRCm38)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,770,703 (GRCm38)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,741,536 (GRCm38)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,762,603 (GRCm38)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,676,767 (GRCm38)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,642,389 (GRCm38)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,757,967 (GRCm38)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,677,032 (GRCm38)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,786,322 (GRCm38)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,677,026 (GRCm38)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,695,160 (GRCm38)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,776,188 (GRCm38)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,677,124 (GRCm38)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,820,755 (GRCm38)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,659,137 (GRCm38)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,770,613 (GRCm38)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,695,143 (GRCm38)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,659,130 (GRCm38)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,698,767 (GRCm38)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,795,701 (GRCm38)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,739,330 (GRCm38)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,677,276 (GRCm38)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,693,458 (GRCm38)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,712,748 (GRCm38)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,794,993 (GRCm38)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,676,437 (GRCm38)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,698,803 (GRCm38)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,762,574 (GRCm38)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,718,114 (GRCm38)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,612,723 (GRCm38)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,665,674 (GRCm38)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,776,152 (GRCm38)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,758,032 (GRCm38)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,676,797 (GRCm38)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,719,623 (GRCm38)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,676,710 (GRCm38)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,612,738 (GRCm38)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,820,766 (GRCm38)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,677,113 (GRCm38)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,738,265 (GRCm38)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,770,616 (GRCm38)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,684,042 (GRCm38)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,757,924 (GRCm38)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,747,146 (GRCm38)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,683,910 (GRCm38)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,719,661 (GRCm38)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,738,319 (GRCm38)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,762,727 (GRCm38)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,604,273 (GRCm38)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,786,212 (GRCm38)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,776,191 (GRCm38)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,758,059 (GRCm38)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,820,723 (GRCm38)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,721,576 (GRCm38)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,604,191 (GRCm38)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,709,004 (GRCm38)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,712,672 (GRCm38)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,718,228 (GRCm38)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,659,143 (GRCm38)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,709,556 (GRCm38)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,709,555 (GRCm38)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,741,513 (GRCm38)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,757,955 (GRCm38)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,741,519 (GRCm38)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,719,628 (GRCm38)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,612,638 (GRCm38)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,747,149 (GRCm38)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,697,878 (GRCm38)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,702,457 (GRCm38)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,782,846 (GRCm38)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,722,914 (GRCm38)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,741,523 (GRCm38)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,659,062 (GRCm38)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,676,518 (GRCm38)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,739,413 (GRCm38)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,738,217 (GRCm38)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,738,316 (GRCm38)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,702,560 (GRCm38)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,820,761 (GRCm38)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,677,006 (GRCm38)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,605,529 (GRCm38)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,676,566 (GRCm38)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,735,096 (GRCm38)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,709,058 (GRCm38)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,708,023 (GRCm38)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,612,700 (GRCm38)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,709,080 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACTCTGCCCTCAACATACAG -3'
(R):5'- TAGAGTAAGTTCATCACCATCAACC -3'

Sequencing Primer
(F):5'- AGAGTGAGACCATTCTTAAGGTG -3'
(R):5'- GTTCATCACCATCAACCAACTCATAG -3'

Posted On

2019-06-26