Mutagenetix > Incidental Mutation

Incidental Mutation 'R8140:Vmn2r103'

632503

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r103

Ensembl Gene

ENSMUSG00000091771

Gene Name

vomeronasal 2, receptor 103

Synonyms

EG627636

MMRRC Submission

067568-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R8140 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19773363-19812536 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19811796 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 611 (T611S)

Ref Sequence

ENSEMBL: ENSMUSP00000126756 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172203]

AlphaFold

E9PWW0

Predicted Effect

probably damaging
Transcript: ENSMUST00000172203
AA Change: T611S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000126756
Gene: ENSMUSG00000091771
AA Change: T611S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	449	1.3e-37	PFAM
Pfam:NCD3G	509	562	3.5e-22	PFAM
Pfam:7tm_3	595	830	1.1e-51	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.6%
10x: 98.6%
20x: 93.9%

Validation Efficiency

97% (64/66)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	T	A	5: 63,898,611	V230E	possibly damaging	Het
Amotl1	G	A	9: 14,572,715		probably null	Het
Atp7b	C	A	8: 22,028,560	E87D	probably damaging	Het
Bcat2	C	T	7: 45,588,351	P347L	probably damaging	Het
Brox	A	T	1: 183,293,873		probably null	Het
Cd37	A	G	7: 45,238,535	I58T	probably damaging	Het
Cep295	A	T	9: 15,341,533	M333K	probably benign	Het
Chtop	A	G	3: 90,505,393		probably null	Het
Cpa2	A	T	6: 30,544,905	K54N	probably benign	Het
Cpa6	A	G	1: 10,325,294	S383P	probably damaging	Het
Dnah7a	A	T	1: 53,501,589	I2542N	probably benign	Het
Eif5b	G	A	1: 38,051,276	V1179I	probably benign	Het
Erbin	T	C	13: 103,920,294		probably null	Het
Fastkd5	A	T	2: 130,615,250	D473E	possibly damaging	Het
Fchsd1	T	C	18: 37,964,342	E372G	probably damaging	Het
Fgl1	T	C	8: 41,200,609		probably null	Het
Fzd8	T	G	18: 9,213,797	V293G	probably damaging	Het
Gm4787	T	A	12: 81,378,151	H411L	probably benign	Het
Gm49380	G	T	9: 44,111,972	D326E	probably benign	Het
Hcrtr1	G	A	4: 130,135,290	R240C	probably damaging	Het
Hdac5	A	T	11: 102,197,355	Y948N	probably damaging	Het
Hepacam	A	G	9: 37,383,871	S301G	probably benign	Het
Htra4	A	T	8: 25,030,558	D362E	possibly damaging	Het
Ighv1-9	G	A	12: 114,583,741	P60L	probably damaging	Het
Kcnq3	A	G	15: 65,995,541	I751T	probably damaging	Het
Magi3	T	C	3: 104,034,086	Y851C	probably damaging	Het
Mefv	A	G	16: 3,713,635	S470P	probably benign	Het
Mfsd2a	C	T	4: 122,949,298	V397I	probably benign	Het
Mroh1	C	A	15: 76,433,873	H867N	probably benign	Het
Mthfd1l	C	T	10: 4,007,745	R261*	probably null	Het
Myo3a	A	G	2: 22,407,346	I725M	probably damaging	Het
Neb	T	C	2: 52,209,540	D4766G	possibly damaging	Het
Nek11	G	T	9: 105,392,957	P22Q	probably damaging	Het
Olfr1179	T	C	2: 88,402,113	T274A	possibly damaging	Het
Olfr1497	A	T	19: 13,795,239	V124E	possibly damaging	Het
Olfr178	T	C	16: 58,889,585	T212A	probably benign	Het
Peg10	C	G	6: 4,756,113	Q230E	unknown	Het
Pipox	T	C	11: 77,883,909	D116G	probably benign	Het
Pkd1l2	C	T	8: 117,047,497	R993H	probably benign	Het
Pkdrej	A	C	15: 85,818,410	N1108K	probably damaging	Het
Polr2a	C	T	11: 69,746,376	R291Q	probably benign	Het
Pomt1	T	A	2: 32,244,297	Y277N	probably damaging	Het
Rasal2	A	G	1: 157,299,235	S78P	probably damaging	Het
Rgl1	A	T	1: 152,557,501	L171Q	probably damaging	Het
Sfta2	A	G	17: 35,601,774	E14G	unknown	Het
Sh3rf3	T	A	10: 59,049,355	S353R	possibly damaging	Het
Slc37a1	A	T	17: 31,322,259	I242F	probably damaging	Het
Srfbp1	T	A	18: 52,488,690	D274E	probably damaging	Het
Syne2	T	A	12: 75,912,353	S685R	possibly damaging	Het
Tenm4	A	C	7: 96,895,176	D2170A	probably damaging	Het
Tnr	A	G	1: 159,863,695	T472A	probably damaging	Het
Tspan9	T	C	6: 127,965,278	H203R	probably damaging	Het
Ttn	T	C	2: 76,771,651	T18556A	possibly damaging	Het
Usp25	T	A	16: 77,071,681	Y323*	probably null	Het
Usp31	G	T	7: 121,649,026	R1065S	possibly damaging	Het
Wdfy4	A	G	14: 33,142,360	V552A		Het
Zap70	G	T	1: 36,771,181	R124L	possibly damaging	Het
Zfand6	A	T	7: 84,632,749	S91T	possibly damaging	Het

Other mutations in Vmn2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00333:Vmn2r103	APN	17	19793102	missense	probably damaging	0.98
IGL00939:Vmn2r103	APN	17	19794965	missense	probably benign	0.00
IGL01120:Vmn2r103	APN	17	19792997	missense	probably benign	0.06
IGL01403:Vmn2r103	APN	17	19792967	missense	probably benign
IGL01404:Vmn2r103	APN	17	19812434	missense	probably damaging	1.00
IGL01713:Vmn2r103	APN	17	19794068	missense	probably damaging	1.00
IGL01802:Vmn2r103	APN	17	19799208	missense	probably benign
IGL02251:Vmn2r103	APN	17	19793969	missense	possibly damaging	0.84
IGL02466:Vmn2r103	APN	17	19773369	missense	probably benign
IGL02555:Vmn2r103	APN	17	19811611	missense	probably damaging	1.00
IGL02668:Vmn2r103	APN	17	19794127	missense	probably benign	0.03
IGL02715:Vmn2r103	APN	17	19793956	missense	probably damaging	0.97
IGL02735:Vmn2r103	APN	17	19812248	missense	probably benign	0.27
IGL03101:Vmn2r103	APN	17	19773520	missense	probably damaging	0.98
R0003:Vmn2r103	UTSW	17	19811979	missense	probably damaging	0.99
R0052:Vmn2r103	UTSW	17	19811641	missense	probably benign	0.01
R0375:Vmn2r103	UTSW	17	19792859	missense	probably benign	0.06
R0375:Vmn2r103	UTSW	17	19793464	missense	probably benign	0.12
R0755:Vmn2r103	UTSW	17	19773568	missense	probably benign	0.01
R0837:Vmn2r103	UTSW	17	19793927	missense	probably damaging	0.99
R1345:Vmn2r103	UTSW	17	19794247	missense	probably damaging	1.00
R1396:Vmn2r103	UTSW	17	19792968	missense	probably benign
R1488:Vmn2r103	UTSW	17	19793660	missense	probably damaging	0.97
R1533:Vmn2r103	UTSW	17	19773400	missense	probably benign	0.01
R1590:Vmn2r103	UTSW	17	19794234	missense	probably benign
R1928:Vmn2r103	UTSW	17	19811767	missense	possibly damaging	0.95
R1942:Vmn2r103	UTSW	17	19812300	missense	probably benign	0.02
R2071:Vmn2r103	UTSW	17	19793794	missense	probably benign
R2219:Vmn2r103	UTSW	17	19793647	missense	probably damaging	1.00
R2442:Vmn2r103	UTSW	17	19773531	missense	probably benign	0.00
R2889:Vmn2r103	UTSW	17	19793600	missense	probably damaging	1.00
R3762:Vmn2r103	UTSW	17	19812149	missense	probably damaging	0.98
R4014:Vmn2r103	UTSW	17	19793604	missense	possibly damaging	0.67
R4331:Vmn2r103	UTSW	17	19794233	missense	probably benign	0.00
R4630:Vmn2r103	UTSW	17	19793696	missense	probably benign	0.04
R4631:Vmn2r103	UTSW	17	19793696	missense	probably benign	0.04
R4632:Vmn2r103	UTSW	17	19793696	missense	probably benign	0.04
R4660:Vmn2r103	UTSW	17	19811815	missense	probably damaging	1.00
R4801:Vmn2r103	UTSW	17	19795076	missense	probably benign	0.06
R4802:Vmn2r103	UTSW	17	19795076	missense	probably benign	0.06
R4931:Vmn2r103	UTSW	17	19811769	missense	probably benign	0.01
R4995:Vmn2r103	UTSW	17	19773511	missense	probably benign	0.14
R5309:Vmn2r103	UTSW	17	19793034	missense	probably benign	0.01
R5312:Vmn2r103	UTSW	17	19793034	missense	probably benign	0.01
R5329:Vmn2r103	UTSW	17	19812171	missense	probably damaging	1.00
R5611:Vmn2r103	UTSW	17	19793642	missense	probably damaging	0.99
R5684:Vmn2r103	UTSW	17	19792989	missense	probably benign	0.02
R5715:Vmn2r103	UTSW	17	19794939	missense	probably benign	0.17
R5907:Vmn2r103	UTSW	17	19812453	missense	possibly damaging	0.67
R6029:Vmn2r103	UTSW	17	19794216	nonsense	probably null
R6114:Vmn2r103	UTSW	17	19812325	missense	probably damaging	0.99
R6285:Vmn2r103	UTSW	17	19812144	missense	probably benign
R6292:Vmn2r103	UTSW	17	19793604	missense	possibly damaging	0.67
R6334:Vmn2r103	UTSW	17	19794082	missense	probably damaging	0.97
R6501:Vmn2r103	UTSW	17	19811904	missense	probably benign	0.29
R6710:Vmn2r103	UTSW	17	19811977	missense	probably damaging	1.00
R6774:Vmn2r103	UTSW	17	19773511	missense	probably benign	0.14
R6981:Vmn2r103	UTSW	17	19793477	missense	probably benign	0.00
R7768:Vmn2r103	UTSW	17	19812052	missense	probably damaging	0.99
R7816:Vmn2r103	UTSW	17	19794214	missense	probably benign	0.06
R7885:Vmn2r103	UTSW	17	19793123	missense	probably benign	0.25
R8002:Vmn2r103	UTSW	17	19799249	missense	probably damaging	1.00
R8031:Vmn2r103	UTSW	17	19793497	missense	probably benign	0.00
R8186:Vmn2r103	UTSW	17	19811943	missense	probably damaging	1.00
R8559:Vmn2r103	UTSW	17	19812384	missense	probably benign	0.01
R9413:Vmn2r103	UTSW	17	19811896	missense	possibly damaging	0.54
R9591:Vmn2r103	UTSW	17	19811659	missense	possibly damaging	0.70
R9652:Vmn2r103	UTSW	17	19793765	missense	probably benign	0.01
R9680:Vmn2r103	UTSW	17	19799263	nonsense	probably null
R9743:Vmn2r103	UTSW	17	19812213	missense	probably damaging	1.00
Z1088:Vmn2r103	UTSW	17	19795047	missense	probably benign	0.08

Predicted Primers

PCR Primer
(F):5'- TTGGACTCCTGTGTTCATGATAAC -3'
(R):5'- TGGCCAACACAGTAGCAAG -3'

Sequencing Primer
(F):5'- CTCACAAGATGTATACCAGTGTGTG -3'
(R):5'- CACAGTAGCAAGAGCCATAGTG -3'

Posted On

2020-06-30